Vector: pHAGE-CMV-fullEF1a-IRES-ZsGreen

This vector is NOT available from the PlasmID Repository and is provided here only as a reference. Please contact if you have any questions.
Name: pHAGE-CMV-fullEF1a-IRES-ZsGreen
Description: Lentiviral vector with internal EF1a promoter, has IRES-ZsGreen (IRES-fluorescent tag); amp resistance; restriction enzyme cloning.
Synonyms: pHAGE-CMV-fullEF1a-IRES-ZsGreen-W, pHAGE-CMV-EF1a-IRES-ZsGreen, pHAGE-CMV-fullEF1a-IRES-ZsGreen-W
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8346
Properties: fluorescent marker, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Vector Map: pHAGE-fullEF1a-MCS-IZsGreen.pdf
Vector Sequence: pHAGE-fullEF1a-MCS-IZsGreen.txt
Comments: ZsGreen reported to be like GFP but brighter (statement not evaluated at the DNA Resource Core).


Type Name Description Start Position End Position
viral LTR LTR viral long terminal repeat (LTR) 0 0
viral response element RRE rev response element 0 0
intervening sequence cpPu central polypurine track 0 0
promoter fEF1ap EF1a promoter (full-length) 0 0
IRES IRES internal ribosome entry site (IRES) 0 0
fluorescent marker ZsGreen ZsGreen fluorescent coding sequence (reportedly like GFP but brighter) 0 0
MCS MCS MCS (between fEF1 promoter and IRES is NotI, NheI, XbaI, BamHI) 0 0
viral LTR dU3LTR viral long terminal repeat (LTR) 0 0
intervening sequence 3'Flank 3' flanking fragment 0 0
selectable marker ampR ampicillin resistance gene 0 0
bacterial origin ColE1 ori ColE1-type origin of replication 0 0
viral origin SV40 ori SV40 origin (and promoter) 0 0
bacterial operon LacO LacO fragment 0 0
viral response element WPRE WPRE response element 0 0


Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector donor and PI


PMID Title

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