Vector: pAAV-MCS8-IZsGreen

Number of plasmids with this backbone: 1
Name: pAAV-MCS8-IZsGreen
Description: Adeno-associated viral (AAV) vector with CMV promoter, human beta globin intron, SV40 polyA signal, maximum capacity for insertion 1.7 kb, has IRES-ZsGreen (IRES-fluorescent tag) as marker of transduced cells; amp resistance; restriction enzyme cloning.
Synonyms: pAAV-MCS-9-I-ZsGreen, pAAVMCS8-IZsGreen
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 6696
Properties: adeno-associated (AAV) expression, fluorescent marker, mammalian expression, mammalian transduction, multiple cloning site, with tag/fusion/marker
Vector Map: pAAV-MCS8-IZsGreen.pdf
Vector Sequence: pAAV-MCS8-IZsGreen.txt
Comments: Each time the plasmid is replicated and amplified, should check with a SmaI digest to ensure that the full plasmid is intact. XLBlue cells recommended over DH5-alpha. ZsGreen like eGFP but brighter (statement not confirmed at DNA Resource Core).

Features:

Type Name Description Start Position End Position
viral LTR ITR AAV inverted terminal repeat 0 0
promoter CMVp CMV promoter for mammalian expression 0 0
gene fragment bglVS beta globin fragment 0 0
MCS MCS MCS (EcoRI, NotI, NheI, XbaI) 0 0
IRES IRES IRES (internal ribosome entry site) 0 0
bacterial origin ColE1 ColE1-type bacterial origin 0 0
gene fragment wtpA+Mz wtpA+Mz 0 0
viral LTR ITR AAV inverted terminal repeat 0 0
gene fragment LacZ alpha LacZ alpha fragment 0 0
ssDNA origin F1 ori F1 origin for ssDNA production 0 0
selectable marker ampR ampicillin resistance gene 0 0
fluorescent marker ZsGreen ZsGreen open reading frame (like GFP but reportedly brighter) 0 0

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector donor and PI

Publications:

PMID Title

Parent Vector:

Name Comments