Vector: pAAV-MCS6-IZsGreen
Number of plasmids with this backbone: | 1 |
Name: | pAAV-MCS6-IZsGreen |
Description: | Adeno-associated viral (AAV) vector with CMV promoter, SV40 polyA signal, maximum capacity for insertion 2.3 kb, has IRES-ZsGreen (IRES-fluorescent tag) as marker of transduced cells; amp resistance; restriction enzyme cloning. |
Synonyms: | pAAV-MCS-6-I-ZsGreen, pAAVMCS6-IZsGreen |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 6111 |
Properties: | adeno-associated (AAV) expression, fluorescent marker, mammalian expression, mammalian transduction, multiple cloning site, with tag/fusion/marker |
Vector Map: | pAAV-MCS6-IZsGreen.pdf |
Vector Sequence: | pAAV-MCS6-IZsGreen.txt |
Comments: | Each time the plasmid is replicated and amplified, should check with a SmaI digest to ensure that the full plasmid is intact. XLBlue cells recommended over DH5-alpha. ZsGreen like eGFP but brighter (statement not confirmed at DNA Resource Core). |
Type | Name | Description | Start Position | End Position |
viral LTR | ITR | AAV inverted terminal repeat | 0 | 0 |
promoter | CMVp | CMV promoter for mammalian expression | 0 | 0 |
IRES | IRES | IRES (internal ribosome entry site) | 0 | 0 |
fluorescent marker | ZsGreen | ZsGreen open reading frame (like GFP but reportedly brighter) | 0 | 0 |
bacterial origin | ColE1 | ColE1-type bacterial origin | 0 | 0 |
viral LTR | ITR | AAV inverted terminal repeat | 0 | 0 |
gene fragment | LacZ-alpha | LacZ alpha fragment | 0 | 0 |
ssDNA origin | F1 ori | F1 origin for ssDNA production | 0 | 0 |
selectable marker | ampR | ampicillin resistance gene | 0 | 0 |
gene fragment | wtpA+Mz | wtpA+Mz | 0 | 0 |
Authors:
Author Name | Author Type | Creation Date |
Jeng-Shin Lee | Academic Researcher, vector donor and PI |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |