Vector: pAAV-MCS
Number of plasmids with this backbone: | 1 |
Name: | pAAV-MCS |
Description: | Adeno-associated viral (AAV) vector with CMV promoter, beta globin intron and polyA signal, maximum capacity for insertion 2.3 kb; amp resistance; restriction enzyme cloning. |
Synonyms: | pAAVMCS |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 6150 |
Properties: | mammalian transduction |
Vector Map: | pAAV-MCS.pdf |
Vector Sequence: | pAAV-MCS.txt |
Comments: | Each time the plasmid is replicated and amplified, should check with a SmaI digest to ensure that the full plasmid is intact. XLBlue cells recommended instead of DH5-alpha. |
Type | Name | Description | Start Position | End Position |
viral LTR | ITR | AAV inverted terminal repeat | 0 | 0 |
promoter | CMVp | CMV promoter for mammalian expression | 0 | 0 |
gene fragment | bgexII | beta-globin exon II fragment | 0 | 0 |
gene fragment | bhIVSII | bgIVSII fragment | 0 | 0 |
MCS | MCS | MCS (EcoRI, NotI, BamHI, SalI, XhoI, BlgII) | 0 | 0 |
gene fragment | bgexIII | bgexIII fragment | 0 | 0 |
viral LTR | ITR | AAV inverted terminal repeat | 0 | 0 |
ssDNA origin | F1 ori | F1 origin for ssDNA production | 0 | 0 |
selectable marker | ampR | ampicillin resistance gene | 0 | 0 |
bacterial origin | ColE1 | ColE1-type bacterial origin | 0 | 0 |
Authors:
Author Name | Author Type | Creation Date |
Jeng-Shin Lee | Academic Researcher, vector donor and PI |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |