Vector: pAAV-MCS

Number of plasmids with this backbone: 1
Name: pAAV-MCS
Description: Adeno-associated viral (AAV) vector with CMV promoter, beta globin intron and polyA signal, maximum capacity for insertion 2.3 kb; amp resistance; restriction enzyme cloning.
Synonyms: pAAVMCS
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 6150
Properties: mammalian transduction
Vector Map: pAAV-MCS.pdf
Vector Sequence: pAAV-MCS.txt
Comments: Each time the plasmid is replicated and amplified, should check with a SmaI digest to ensure that the full plasmid is intact. XLBlue cells recommended instead of DH5-alpha.

Features:

Type Name Description Start Position End Position
viral LTR ITR AAV inverted terminal repeat 0 0
promoter CMVp CMV promoter for mammalian expression 0 0
gene fragment bgexII beta-globin exon II fragment 0 0
gene fragment bhIVSII bgIVSII fragment 0 0
MCS MCS MCS (EcoRI, NotI, BamHI, SalI, XhoI, BlgII) 0 0
gene fragment bgexIII bgexIII fragment 0 0
viral LTR ITR AAV inverted terminal repeat 0 0
ssDNA origin F1 ori F1 origin for ssDNA production 0 0
selectable marker ampR ampicillin resistance gene 0 0
bacterial origin ColE1 ColE1-type bacterial origin 0 0

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector donor and PI

Publications:

PMID Title

Parent Vector:

Name Comments