Vector: pAAV-GFP

Number of plasmids with this backbone: 1
Name: pAAV-GFP
Description: Adeno-associated viral (AAV) vector, CMV promoter drives GFP fluorescent marker; amp resistance; restriction enzyme cloning.
Synonyms: pAAVGFP
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 6940
Properties: mammalian transduction
Vector Map: pAAV-GFP.pdf
Vector Sequence: pAAV-GFP.txt
Comments: Each time the plasmid is replicated and amplified, should check with a SmaI digest to ensure that the full plasmid is intact. XLBlue cells recommended instead of DH5-alpha.

Features:

Type Name Description Start Position End Position
gene fragment hbg IVSII hbg IVSII region 0 0
fluorescent marker GFP green fluorescent protein 0 0
MCS MCS MCS (NotI, SalI, XhoI, BglII) 0 0
viral LTR ITR AAV inverted terminal repeat 0 0
promoter CMVp CMV promoter for mammalian expression 0 0
ssDNA origin F1 ori F1 origin for ssDNA replication 0 0
selectable marker ampR ampicillin resistance gene 0 0
bacterial origin ColE1 ori ColE1-type bacterial origin 0 0
gene fragment hbg exIII human beta-globin exon fragment 0 0
viral LTR ITR AAV inverted terminal repeat 0 0

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector donor and PI

Publications:

PMID Title

Parent Vector:

Name Comments