Vector: pLPS-3'EGFP

Number of plasmids with this backbone: 232
Name: pLPS-3'EGFP
Description: Mammalian expression vector with CMV promoter that adds C-terminal fluorescent (EGFP) tag; kanamycin resistance (neomycin for mammalian expression); recombinational cloning.
Synonyms: pLPS-3pEGFP, pLPS3'EGFP, pLPS-3p-EGFP
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 4900
Properties: Creator, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning, ssDNA production, with tag/fusion/marker
Vector Map: pLPS3pEGFP.pdf
Vector Sequence: pLPS3pEGFP.txt
Comments: Clontech vector that has since been discontinued

Features:

Type Name Description Start Position End Position
promoter CMV pr CMV promoter for mammalian expression 1 589
recombination site LoxP LoxP site for recombinational cloning 597 630
promoter pr Bacterial promoter 632 757
splice acceptor site SA splice acceptor (SA) site 784 835
tag EGFP EGFP enhanced green fluorescent protein (no ATF) 846 1559
poly-A signal SV40 polyA SV40 polyadenylation signal sequence 1713 1718
poly-A signal SV40 polyA SV40 polyadenylation signal sequence 1742 1747
ssDNA origin f1 ori f1 origin for ssDNA production 1810 2265
promoter pr bacterial promoter for kanR 2327 2362
viral origin SV40 ori SV40 origin of replication 2606 2741
promoter SV40 pr SV40 early promoter (for neoR) 2662 2668
selectable marker kanR kanamycin/neomycin resistance gene (neomycin phosphotransferase) 2790 3584
poly-A signal HSV TK polyA HSV thymidine kinase (TK) polyadenylation signal sequence 3820 3825
poly-A signal HSV TK polyA HSV thymidine kinase (TK) polyadenylation signal sequence 3833 3838
bacterial origin pUC ori pUC origin of replication 4169 4812

Authors:

Author Name Author Type Creation Date
BD Biosciences Clontech Commercial supplier

Publications:

PMID Title

Parent Vector:

Name Comments