Vector: pST76K-SR

Number of plasmids with this backbone: 1
Name: pST76K-SR
Description: Shuttle vector for RecA-mediated BAC mutagenesis, ts for DNA replication; kanamycin resistance; in vivo selection for cointegrants followed by resolution to manipulate BACs.
Synonyms: pST76K_SR
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 6326
Properties: BAC mutagenesis, RecA mutagenesis, multiple cloning site, mutagenesis vector
Vector Map: pST76K-SR.pdf
Vector Sequence: pST76K-SR.txt
Comments: Donor of the 'empty vector' clone of this vector provided a protocol for use; see clone description

Features:

Type Name Description Start Position End Position
MCS MCS MCS (SacI, KpnI, SmaI, SmaI, BamHI) between sacB and RecA 0 0
recombination site FRT FRT site-specific recombination recognition sequence 17 50
negative selection marker sacB sacB (levansucrase) death/suicide gene 217 1635
gene fragment RecA E. coli RecA protein 1999 3060
selectable marker repTS repTS (confers temperature sensitivity to DNA replication) 3295 4245
selectable marker kanR neoR gene for kanamycin resistance 5381 6175

Authors:

Author Name Author Type Creation Date
G Posfai Academic researcher, made the vector

Publications:

PMID Title
15890938 vLIP, a viral lipase homologue, is a virulence factor of Marek's disease virus
10933677 Fast screening procedures for random transposon libraries of cloned herpesvirus genomes: mutational analysis of human cytomegalovirus envelope glycoprotein genes
10888635 Cloning and mutagenesis of the murine gammaherpesvirus 68 genome as an infectious bacterial artificial chromosome

Parent Vector:

Name Comments