Vector: pST76K-SR
Number of plasmids with this backbone: | 1 |
Name: | pST76K-SR |
Description: | Shuttle vector for RecA-mediated BAC mutagenesis, ts for DNA replication; kanamycin resistance; in vivo selection for cointegrants followed by resolution to manipulate BACs. |
Synonyms: | pST76K_SR |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 6326 |
Properties: | BAC mutagenesis, RecA mutagenesis, multiple cloning site, mutagenesis vector |
Vector Map: | pST76K-SR.pdf |
Vector Sequence: | pST76K-SR.txt |
Comments: | Donor of the 'empty vector' clone of this vector provided a protocol for use; see clone description |
Type | Name | Description | Start Position | End Position |
MCS | MCS | MCS (SacI, KpnI, SmaI, SmaI, BamHI) between sacB and RecA | 0 | 0 |
recombination site | FRT | FRT site-specific recombination recognition sequence | 17 | 50 |
negative selection marker | sacB | sacB (levansucrase) death/suicide gene | 217 | 1635 |
gene fragment | RecA | E. coli RecA protein | 1999 | 3060 |
selectable marker | repTS | repTS (confers temperature sensitivity to DNA replication) | 3295 | 4245 |
selectable marker | kanR | neoR gene for kanamycin resistance | 5381 | 6175 |
Authors:
Author Name | Author Type | Creation Date |
G Posfai | Academic researcher, made the vector |
Publications:
PMID | Title |
15890938 | vLIP, a viral lipase homologue, is a virulence factor of Marek's disease virus |
10933677 | Fast screening procedures for random transposon libraries of cloned herpesvirus genomes: mutational analysis of human cytomegalovirus envelope glycoprotein genes |
10888635 | Cloning and mutagenesis of the murine gammaherpesvirus 68 genome as an infectious bacterial artificial chromosome |
Parent Vector:
Name | Comments |