Vector: pNTAP-TSR
Number of plasmids with this backbone: | 1 |
Name: | pNTAP-TSR |
Description: | Vector for BAC mutagenesis via Red recombination (kanR excised upon I-SceI and Rec E/T induction) with disrupted affinity tag (synthetic ORF eliminates nucleotide repeats present in wild-type); kanamycin resistance; restriction enzyme cloning. |
Synonyms: | pSP72-PTP-KanR, pSP72-syn-NTAP-AphAI, pSP72-PTP-AphAI |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 3968 |
Properties: | BAC mutagenesis, I-SceI site, Red recombination, bacterial expression, multiple cloning site, mutagenesis vector, with tag/fusion/marker |
Vector Map: | pSP72-PTP-NTAP-ISceI.pdf |
Vector Sequence: | pSP72-syn-ProteinC-NTAP-AphAI.txt |
Comments: | PTP N-terminal TAP tag (see PMID 16278461) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human |
Features:
Type | Name | Description | Start Position | End Position |
Authors:
Author Name | Author Type | Creation Date |
Publications:
PMID | Title |
16526409 | Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli |
10504710 | A generic protein purification method for protein complex characterization and proteome exploration |
17634236 | Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65. |
Parent Vector:
Name | Comments |