Vector: pNTAP-TSR

Number of plasmids with this backbone: 1
Description: Vector for BAC mutagenesis via Red recombination (kanR excised upon I-SceI and Rec E/T induction) with disrupted affinity tag (synthetic ORF eliminates nucleotide repeats present in wild-type); kanamycin resistance; restriction enzyme cloning.
Synonyms: pSP72-PTP-KanR, pSP72-syn-NTAP-AphAI, pSP72-PTP-AphAI
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 3968
Properties: BAC mutagenesis, I-SceI site, Red recombination, bacterial expression, multiple cloning site, mutagenesis vector, with tag/fusion/marker
Vector Map: pSP72-PTP-NTAP-ISceI.pdf
Vector Sequence: pSP72-syn-ProteinC-NTAP-AphAI.txt
Comments: PTP N-terminal TAP tag (see PMID 16278461) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human


Type Name Description Start Position End Position


Author Name Author Type Creation Date


PMID Title
16526409 Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
10504710 A generic protein purification method for protein complex characterization and proteome exploration
17634236 Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65.

Parent Vector:

Name Comments