Vector: pNTAP-TSR

Number of plasmids with this backbone: 1
Name: pNTAP-TSR
Description: Vector for BAC mutagenesis via Red recombination (kanR excised upon I-SceI and Rec E/T induction) with disrupted affinity tag (synthetic ORF eliminates nucleotide repeats present in wild-type); kanamycin resistance; restriction enzyme cloning.
Synonyms: pSP72-PTP-KanR, pSP72-syn-NTAP-AphAI, pSP72-PTP-AphAI
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 3968
Properties: BAC mutagenesis, I-SceI site, Red recombination, bacterial expression, multiple cloning site, mutagenesis vector, with tag/fusion/marker
Vector Map: pSP72-PTP-NTAP-ISceI.pdf
Vector Sequence: pSP72-syn-ProteinC-NTAP-AphAI.txt
Comments: PTP N-terminal TAP tag (see PMID 16278461) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human

Features:

Type Name Description Start Position End Position

Authors:

Author Name Author Type Creation Date

Publications:

PMID Title
16526409 Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
10504710 A generic protein purification method for protein complex characterization and proteome exploration
17634236 Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65.

Parent Vector:

Name Comments