Vector: pJK-NTAP
Number of plasmids with this backbone: | 1 |
Name: | pJK-NTAP |
Description: | Cloning vector with Protein C NTAP tag ORF (synthetic ORF eliminates nucleotide repeats present in wild-type); amp resistance; restriction enzyme cloning |
Synonyms: | pSP72-syn-NTAP, pSP72-PTP |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 2930 |
Properties: | cloning vector, in vitro transcription, multiple cloning site, with tag/fusion/marker |
Vector Map: | pSP72-syn-Protein_C-NTAP.pdf |
Vector Sequence: | pSP72-syn-Protein_C-NTAP.txt |
Comments: | PTP N-terminal TAP tag (see PMID 16278461) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human |
Type | Name | Description | Start Position | End Position |
primer site | SP6 | SP6 primer bind site/promoter | 1 | 1 |
MCS | MCS 1 | MCS (XhoI, StuI) | 5 | 38 |
tag | PTP NTAP | synthetic (removes repeats, codon optimized for human) PTP NTAP tag CDS | 31 | 507 |
tag | IgG 1 | IgG binding domain 1 (from split syn-Protein C-NTAP affinity tag) | 61 | 234 |
MCS | I-SceI site | I-SceI endonuclease cleavage site | 263 | 280 |
MCS | MCS 2 | MCS (XbaI, BamHI, SmaI, KpnI, EcoRI, ClaI, BglII) | 509 | 553 |
primer site | T7 | T7 primer bind site and promoter | 567 | 586 |
bacterial origin | ColE1 ori | ColE1-type origin of replication | 826 | 1508 |
tag | IgG 2 | IgG binding domain 2 (from split syn-Protein C-NTAP affinity tag) | 1273 | 1458 |
tag | Protein C epitope | Protein C epitope (from split syn-Protein C-NTAP affinity tag) | 1510 | 1545 |
promoter | T7 | T7 primer bind site, promoter, trxn start | 1605 | 1624 |
selectable marker | ampR | ampicillin resistance gene | 1605 | 2265 |
bacterial origin | ColE1 ori | ColE1-type origin of replication | 1864 | 2546 |
selectable marker | ampR | ampicillin resistance gene | 2644 | 3313 |
primer site | SP6 | SP6 primer bind site/promoter | 3952 | 1 |
Authors:
Author Name | Author Type | Creation Date |
Publications:
PMID | Title |
10504710 | A generic protein purification method for protein complex characterization and proteome exploration |
17634236 | Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65. |
Parent Vector:
Name | Comments |