Vector: pJK-NTAP

Number of plasmids with this backbone: 1
Name: pJK-NTAP
Description: Cloning vector with Protein C NTAP tag ORF (synthetic ORF eliminates nucleotide repeats present in wild-type); amp resistance; restriction enzyme cloning
Synonyms: pSP72-syn-NTAP, pSP72-PTP
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 2930
Properties: cloning vector, in vitro transcription, multiple cloning site, with tag/fusion/marker
Vector Map: pSP72-syn-Protein_C-NTAP.pdf
Vector Sequence: pSP72-syn-Protein_C-NTAP.txt
Comments: PTP N-terminal TAP tag (see PMID 16278461) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human

Features:

Type Name Description Start Position End Position
primer site SP6 SP6 primer bind site/promoter 1 1
MCS MCS 1 MCS (XhoI, StuI) 5 38
tag PTP NTAP synthetic (removes repeats, codon optimized for human) PTP NTAP tag CDS 31 507
tag IgG 1 IgG binding domain 1 (from split syn-Protein C-NTAP affinity tag) 61 234
MCS I-SceI site I-SceI endonuclease cleavage site 263 280
MCS MCS 2 MCS (XbaI, BamHI, SmaI, KpnI, EcoRI, ClaI, BglII) 509 553
primer site T7 T7 primer bind site and promoter 567 586
bacterial origin ColE1 ori ColE1-type origin of replication 826 1508
tag IgG 2 IgG binding domain 2 (from split syn-Protein C-NTAP affinity tag) 1273 1458
tag Protein C epitope Protein C epitope (from split syn-Protein C-NTAP affinity tag) 1510 1545
promoter T7 T7 primer bind site, promoter, trxn start 1605 1624
selectable marker ampR ampicillin resistance gene 1605 2265
bacterial origin ColE1 ori ColE1-type origin of replication 1864 2546
selectable marker ampR ampicillin resistance gene 2644 3313
primer site SP6 SP6 primer bind site/promoter 3952 1

Authors:

Author Name Author Type Creation Date

Publications:

PMID Title
10504710 A generic protein purification method for protein complex characterization and proteome exploration
17634236 Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65.

Parent Vector:

Name Comments