Vector: pSP72 syn-CTAP

Number of plasmids with this backbone: 1
Name: pSP72 syn-CTAP
Description: Cloning vector with CTAP tag ORF (synthetic ORF eliminates nucleotide repeats present in wild-type form), SP6 and T7 promoters; amp resistance; restriction enzyme cloning.
Synonyms: pSP72-synCTAP, pSP72synCTAP, pSP72-syn-CTAP
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 3017
Properties: cloning vector, in vitro transcription, multiple cloning site, with tag/fusion/marker
Vector Map: pSP72synCTAP.pdf
Vector Sequence: pSP72synCTAP.txt
Comments: C-terminal TAP tag (see PMID 10504710) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human

Features:

Type Name Description Start Position End Position
primer site SP6 SP6 primer bind site/promoter 1 1
MCS MCS1 MCS (XbaI, BamHI, SmaI, KpnI) 41 59
tag syn-CTAP synthetic (removes repeats, codon optimized for human) CTAP affinity tag 61 582
MCS MCS2 MCS (EcoRI, ClaI, BglII) 623 640
primer site T7 T7 primer bind site/promoter 654 673
bacterial origin ColE1 ori ColE1-type origin of replication 913 1595
selectable marker ampR ampicillin resistance gene 1693 2352

Authors:

Author Name Author Type Creation Date

Publications:

PMID Title
10504710 A generic protein purification method for protein complex characterization and proteome exploration

Parent Vector:

Name Comments