Vector: pSP72 syn-CTAP

Number of plasmids with this backbone: 1
Name: pSP72 syn-CTAP
Description: Cloning vector with CTAP tag ORF (synthetic ORF eliminates nucleotide repeats present in wild-type form), SP6 and T7 promoters; amp resistance; restriction enzyme cloning.
Synonyms: pSP72-synCTAP, pSP72synCTAP, pSP72-syn-CTAP
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 3017
Properties: cloning vector, in vitro transcription, multiple cloning site, with tag/fusion/marker
Vector Map: pSP72synCTAP.pdf
Vector Sequence: pSP72synCTAP.txt
Comments: C-terminal TAP tag (see PMID 10504710) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human


Type Name Description Start Position End Position
primer site SP6 SP6 primer bind site/promoter 1 1
MCS MCS1 MCS (XbaI, BamHI, SmaI, KpnI) 41 59
tag syn-CTAP synthetic (removes repeats, codon optimized for human) CTAP affinity tag 61 582
MCS MCS2 MCS (EcoRI, ClaI, BglII) 623 640
primer site T7 T7 primer bind site/promoter 654 673
bacterial origin ColE1 ori ColE1-type origin of replication 913 1595
selectable marker ampR ampicillin resistance gene 1693 2352


Author Name Author Type Creation Date


PMID Title
10504710 A generic protein purification method for protein complex characterization and proteome exploration

Parent Vector:

Name Comments