Vector: pIRES2 lac smGFP
Number of plasmids with this backbone: | 1 |
Name: | pIRES2 lac smGFP |
Description: | Mammalian expression vector with fluorescent tag (soluble modified GFP, smGFP), CMV promoter, lac promoter; kanamycin resistance (neomycin for mammalian selection); restriction enzyme cloning. |
Synonyms: | pIRES lac smGFP, pIRES-lac-smGFP, pIRES2-lac-smGFP |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 5544 |
Properties: | bacterial expression, fluorescent marker, mammalian expression, ssDNA production, with tag/fusion/marker |
Vector Map: | pIRES-lac-smGFP.pdf |
Vector Sequence: | pIRES-lac-smGFP.txt |
Comments: | Not clear pIRES2 or pIRES parent vector; clone donor notes smGFP (see PubMed ID 9484447) less toxic than GFP or EGFP in mammalian cells |
Type | Name | Description | Start Position | End Position |
promoter | CMV-IE pr | CMV immediate early promoter | 1 | 589 |
IRES | IRES | internal ribosome entry site (IRES) | 667 | 1250 |
promoter | lac pr | lac promoter (prokaryotic expression) | 1251 | 1366 |
tag | smGFP | soluble modified GFP | 1367 | 2188 |
poly-A signal | polyA | polyadenylation signal sequence | 2312 | 2876 |
ssDNA origin | f1 ori | f1 origin for ssDNA production | 2470 | 2910 |
promoter | SV40 pr | SV40 early promoter | 3209 | 3405 |
selectable marker | neoR | neomycin resistance gene | 3440 | 4234 |
bacterial origin | ColE1 ori | ColE1-type origin of replication | 4766 | 5448 |
Authors:
Author Name | Author Type | Creation Date |
Publications:
PMID | Title |
9484447 | Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants |
Parent Vector:
Name | Comments |