Vector: pFASTBAC1 GST PP Cpo

Number of plasmids with this backbone: 1
Name: pFASTBAC1 GST PP Cpo
Description: Transposable baculovirus expression vector for use with Invitrogen Bac-to-Bac system, has polyhedrin promoter, encodes N-terminal GST and Prescission Protease site prior to MCS; amp resistance (gentamicin for recombinant BACmid selection after transposition); standard restriction enyzme or CpoI-based in-frame single-cut directional cloning.
Synonyms: pFASTBAC1-GST-PP-Cpo, pFASTBAC11-GST-PP-CpoI
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 5462
Properties: CpoI enzyme-based directional cloning, baculovirus/insect cell expression, baculovirus/insect cell transposition, multiple cloning site, with tag/fusion/marker
Vector Map: pFASTBAC1-GST-PP-Cpo.pdf
Vector Sequence: pFASTBAC1-GST-PP-Cpo.txt
Comments:

Features:

Type Name Description Start Position End Position
ssDNA origin f1 ori f1 origin for ssDNA production 2 457
selectable marker ampR ampicillin resistance gene 589 1449
bacterial origin ColE1 ori ColE1-type origin of replication 1494 2268
transposon fragment Tn7R Tn7 attachment site 2512 2736
selectable marker gentamicinR gentamicin resistance gene 2803 3336
promoter polyhedrin pr polyhedrin promoter for insect cell expression 3905 4033
tag GST-PP GST-PP glutathione-S-transferase with PP (N-terminal to an insert) 4042 4724
protease cleavage site PP precision protease site in GST-PP tag 4702 4724
MCS CpoI CpoI site for in-frame, single-cut, directional cloning (in-frame with GST-PP) 4725 4731
MCS MCS MCS (EcoRI, StuI, SalI, SacI, NotI, XbaI, PstI, XhoI, KpnI, HindIII) 4741 4823
poly-A signal SV40 polyA SV40 polyadenylation signal sequence 4847 5087
transposon fragment Tn7L Tn7 attachment site 5116 5281

Authors:

Author Name Author Type Creation Date

Publications:

PMID Title
8392598 Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli

Parent Vector:

Name Comments