Vector: pDNR-Dual

Number of plasmids with this backbone: 6529
Name: pDNR-Dual
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 4938
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Vector Map: pDNR-Dual.pdf
Vector Sequence: pDNR-Dual_FASTA.txt
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.

Features:

Type Name Description Start Position End Position
recombination site loxP site loxP recombination site 9 42
MCS MCS restriction enzyme-type multiple cloning site 45 129
splice donor site SD site splice donor site 132 136
tag 6xHN tag 6xHN epitope tag 141 176
selectable marker chlR ORF chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
recombination site loxP site loxP recombination site 1015 1048
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
selectable marker ampR ampicillin resistance gene 3103 3963
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883

Authors:

Author Name Author Type Creation Date
BD Biosciences Clontech Commercial

Publications:

PMID Title

Parent Vector:

Name Comments