Vector: pDNR-Dual
Number of plasmids with this backbone: | 6529 |
Name: | pDNR-Dual |
Description: | Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from). |
Synonyms: | |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 4938 |
Properties: | Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker |
Vector Map: | pDNR-Dual.pdf |
Vector Sequence: | pDNR-Dual_FASTA.txt |
Comments: | The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added. |
Type | Name | Description | Start Position | End Position |
recombination site | loxP site | loxP recombination site | 9 | 42 |
MCS | MCS | restriction enzyme-type multiple cloning site | 45 | 129 |
splice donor site | SD site | splice donor site | 132 | 136 |
tag | 6xHN tag | 6xHN epitope tag | 141 | 176 |
selectable marker | chlR ORF | chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) | 986 | 327 |
recombination site | loxP site | loxP recombination site | 1015 | 1048 |
negative selection marker | SacB | SacB negative selection marker gene (sucrose sensitivity) | 1057 | 2972 |
selectable marker | ampR | ampicillin resistance gene | 3103 | 3963 |
bacterial origin | ColE1 | ColE1 (pUC-type) origin of replication | 4111 | 4574 |
primer site | M13 primer | M13 primer sequencing site | 4839 | 4855 |
primer site | T7 primer | T7 RNA Pol primer sequencing site | 4865 | 4883 |
Authors:
Author Name | Author Type | Creation Date |
BD Biosciences Clontech | Commercial |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |