Vector: pFA6a-mRFP1-kanMX6

Number of plasmids with this backbone: 1
Name: pFA6a-mRFP1-kanMX6
Description: Yeast fluorescent (RFP) vector with PTEF promoter; amp resistance (kanamycin for yeast); restriction enzyme cloning.
Synonyms: pFA6a-RFP-kanR
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 4846
Properties: fluorescent marker, multiple cloning site, yeast expression, yeast shuttle
Vector Map: pFA6a-mRFP-KanMX6.pdf
Vector Sequence: pFA6a-mRFP-KanMX6.txt
Comments:

Features:

Type Name Description Start Position End Position
promoter PTEF pr PTEF eukaryotic promoter (inferred from vector family) 0 0
reporter gene RFP red fluorescent protein (FRP) 63 737
selectable marker kanR kanamycin resistance gene (for yeast) 1367 2173
primer site T7 primer site T7 primer binding site 2483 2501
bacterial origin ColEI ori ColE1-type bacterial origin of replication 2742 3424
selectable marker ampR ampicillin resistance gene (bla) 3522 4181

Authors:

Author Name Author Type Creation Date
Erin O'Shea Academic reseracher, vector PI
WK Huh Academic researcher, vector first author

Publications:

PMID Title
14562095 Global analysis of protein localization in budding yeast

Parent Vector:

Name Comments