Vector: pEF1 Cpo V5 His

Number of plasmids with this backbone: 1
Name: pEF1 Cpo V5 His
Description: Mammalian and in vitro expression vector with EF1-alpha promoter and T7, adds C-eterminal V5 then His tag; amp resistance (neomycin for mammalian selection); CpoI-based in-frame, single-cut directional cloning.
Synonyms: pEF1-Cpo-V5-H, pEF1-Cpo-V5-His
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 6121
Properties: CpoI enzyme-based directional cloning, in vitro transcription, mammalian expression, multiple cloning site, ssDNA production, with tag/fusion/marker
Vector Map: pEF1-Cpo-V5-His.pdf
Vector Sequence: pEF1-Cpo-V5-His.txt
Comments: Cloning into CpoI site can be used to create in-frame C-terminal V5, His tagged fusion ORF

Features:

Type Name Description Start Position End Position
promoter EF-1 alpha pr EF-1 alpha promoter (see 12530082 for comparison of EF-1 pr to CMV pr) 470 1653
primer site T7 primer T7 primer bind site 1670 1689
MCS CpoI site CpoI restriction site 1720 1726
tag V5 tag V5 epitope tag ORF 1742 1783
tag His tag His epitope tag ORF 1793 1810
poly-A signal BGH polyA BGH poly A signal sequence 1836 2063
ssDNA origin f1 ori f1 origin for ssDNA production 2109 2537
promoter SV40 pr SV40 promoter/origin of replication 2591 2912
viral origin SV40 ori SV40 promoter/origin of replication 2591 2912
selectable marker neoR neomycin resistance gene (neoR) with G to C change to remove CpoI site 2948 3742
poly-A signal SV40 polyA SV40 polyA signal sequence 3797 4169
primer site M13 rev primer M13 reverse primer bind site 4082 4102
repressor binding site lacO bind site lacO binding site 4108 4130
bacterial origin ColE1 ori ColE1 origin of replication 4470 5152
selectable marker ampR ampicillin resistance gene 5250 5909

Authors:

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