Name: |
pEF1 Cpo Myc His |
Description: |
Mammalian and in vitro expression vector with EF1-alpha promoter and T7, adds C-terminal Myc then His tag; amp resistance (neomycin for mammalian selection); CpoI-based in-frame, single-cut directional cloning. |
Synonyms: |
pEF1-Cpo-MH, pEF1-Cpo-Myc-His |
Type: |
bacterial plasmid |
Form: |
dsDNA |
Size (bp):: |
6112 |
Properties: |
CpoI enzyme-based directional cloning, in vitro transcription, mammalian expression, multiple cloning site, ssDNA production, with tag/fusion/marker |
Vector Map: |
pEF1-Cpo-Myc-His.pdf
|
Vector Sequence: |
pEF1-Cpo-Myc-His.txt |
Comments: |
Cloning into CpoI site can be used to create in-frame C-terminal Myc, His tagged fusion ORF |
Type |
Name |
Description |
Start Position |
End Position |
promoter |
EF-1 alpha pr |
EF-1 alpha promoter (see 12530082 for comparison of EF-1 pr to CMV pr) |
470 |
1653 |
promoter |
T7 |
T7 primer bind site/promoter |
1670 |
1689 |
MCS |
CpoI site |
Cpo I restriction enzyme site |
1720 |
1726 |
tag |
Myc |
Myc epitope tag open reading frame |
1739 |
1768 |
tag |
His tag |
His tag open reading frame |
1784 |
1801 |
poly-A signal |
BGH polyA |
BGH polyA signal sequence |
1827 |
2054 |
ssDNA origin |
f1 ori |
f1 origin for ssDNA production |
2100 |
2528 |
promoter |
SV40 pr |
SV40 promoter/origin of replication |
2582 |
2903 |
viral origin |
SV40 ori |
SV40 promoter/origin of replication |
2582 |
2903 |
selectable marker |
neoR |
neoR (neomycin transferase) with G to C change to remove CpoI site |
2939 |
3733 |
poly-A signal |
SV40 polyA |
SV40 polyadenylation signal sequence |
3788 |
4168 |
primer site |
M13 rev |
M13 reverse primer bind site |
4073 |
4093 |
bacterial origin |
ColE1 ori |
ColE1-type origin of replication |
4461 |
5143 |
selectable marker |
ampR |
ampicillin resistance gene |
5241 |
5900 |