Vector: pEF1 Cpo Myc His

Number of plasmids with this backbone: 1
Name: pEF1 Cpo Myc His
Description: Mammalian and in vitro expression vector with EF1-alpha promoter and T7, adds C-terminal Myc then His tag; amp resistance (neomycin for mammalian selection); CpoI-based in-frame, single-cut directional cloning.
Synonyms: pEF1-Cpo-MH, pEF1-Cpo-Myc-His
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 6112
Properties: CpoI enzyme-based directional cloning, in vitro transcription, mammalian expression, multiple cloning site, ssDNA production, with tag/fusion/marker
Vector Map: pEF1-Cpo-Myc-His.pdf
Vector Sequence: pEF1-Cpo-Myc-His.txt
Comments: Cloning into CpoI site can be used to create in-frame C-terminal Myc, His tagged fusion ORF

Features:

Type Name Description Start Position End Position
promoter EF-1 alpha pr EF-1 alpha promoter (see 12530082 for comparison of EF-1 pr to CMV pr) 470 1653
promoter T7 T7 primer bind site/promoter 1670 1689
MCS CpoI site Cpo I restriction enzyme site 1720 1726
tag Myc Myc epitope tag open reading frame 1739 1768
tag His tag His tag open reading frame 1784 1801
poly-A signal BGH polyA BGH polyA signal sequence 1827 2054
ssDNA origin f1 ori f1 origin for ssDNA production 2100 2528
promoter SV40 pr SV40 promoter/origin of replication 2582 2903
viral origin SV40 ori SV40 promoter/origin of replication 2582 2903
selectable marker neoR neoR (neomycin transferase) with G to C change to remove CpoI site 2939 3733
poly-A signal SV40 polyA SV40 polyadenylation signal sequence 3788 4168
primer site M13 rev M13 reverse primer bind site 4073 4093
bacterial origin ColE1 ori ColE1-type origin of replication 4461 5143
selectable marker ampR ampicillin resistance gene 5241 5900

Authors:

Author Name Author Type Creation Date

Publications:

PMID Title

Parent Vector:

Name Comments