Vector: pJP1563

Number of plasmids with this backbone: 2
Name: pJP1563
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7692
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Vector Map: pJP1563.pdf
Vector Sequence: pJP1563.txt
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP


Type Name Description Start Position End Position
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
selectable marker blastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
recombination site LoxP LoxP site for recombinational cloning 3651 3684
promoter bact pr bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551
selectable marker ampR ampicillin resistance gene (beta-lactamase) 6237 7094


Author Name Author Type Creation Date
Ed Harlow Academic researcher, vector PI
Joseph Pearlberg Academic researcher, vector donor and author


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