Vector: pDONR201

Number of plasmids with this backbone: 6627
Name: pDONR201
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 4470
Properties: Gateway, donor (entry), recombinational cloning
Vector Map: pdonr201_pdonr207_map.pdf
Vector Sequence: pDONR201_FASTA.txt
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.

Features:

Type Name Description Start Position End Position
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275
primer site forward primer recommended forward primer site 300 324
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attL1 "attL recombination site 1 present in recombined (with insert) form only, position is approximate" 332 563
negative selection marker ccdB "ccdB negative selection gene (death cassette), LOST in recombined (with insert) form" 1264 959
selectable marker chlR "chloramphenicol resistance gene, LOST in recombined (with insert) form" 2265 2513
recombination site attL2 "attL recombination site 2 present in recombined (with insert) form only, position is approximate" 2744 2513
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
primer site reverse primer recommended reverse primer site 2769 2792
selectable marker kanR kanamycin resistance gene 2868 3677
bacterial origin ColE1 "ColE1 (pUC-type) origin of replication (modified, low copy number)" 3794 4467

Authors:

Author Name Author Type Creation Date
Invitrogen Commercial

Publications:

PMID Title

Parent Vector:

Name Comments