Clone: ScCD00098000

Clone ID:ScCD00098000
Is Verified:N
Status:AVAILABLE
Source:HIP
Description:
Comments:
Type:cDNA
Verification Method:Insert was fully sequenced in parent vector
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

HIP Clone ID 261303
HIP Master Clone ID 1277
Original Clone ID FLH261303.01X

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 2130 Saccharomyces cerevisiae CLOSED Genomic DNA template YDR146C SWI5 Transcription factor that activates transcription of genes expressed in G1 phase and at the G1/M boundary; localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase
Insert1
Size (bp)2130
SpeciesSaccharomyces cerevisiae
FormatCLOSED
Tissue SourceGenomic DNA template
Species Specific ID YDR146C
Gene SymbolSWI5
Gene NameTranscription factor that activates transcription of genes expressed in G1 phase and at the G1/M boundary; localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase
Keyword

Insert Sequence:

ATGGATACATCAAACTCTTGGTTTGATGCCTCAAAAGTACAAAGCCTAAATTTTGACCTACAAACCAACTCTTATTACTCAAATGCTAGAGGCAGTGACCCTTCGAGTTATGCAATAGAAGGCGAATATAAAACACTAGCAACAGATGATTTAGGCAATATATTGAACCTAAACTATGGTGAAACCAACGAAGTTATAATGAATGAAATAAATGACTTAAACCTTCCGCTGGGACCACTTTCTGATGAAAAATCTGTCAAGGTTTCTACTTTCTCCGAGTTAATAGGAAATGATTGGCAAAGTATGAACTTTGACTTAGAAAATAATTCAAGAGAAGTGACTCTAAATGCAACTAGCTTGTTGAATGAAAATAGATTAAATCAAGACAGTGGTATGACTGTTTATCAAAAAACAATGAGTGATAAGCCCCACGATGAAAAGAAGATTTCCATGGCGGATAATTTATTGTCGACTATAAACAAAAGTGAAATTAATAAAGGCTTCGATAGAAACCTCGGGGAATTACTGTTACAGCAACAACAAGAGTTGCGCGAGCAATTGAGAGCACAACAAGAGGCTAATAAGAAGTTAGAGTTAGAACTCAAACAAACACAATACAAGCAACAACAACTACAGGCTACCTTAGAGAACTCTGATGGTCCACAGTTTTTATCTCCCAAAAGGAAGATCTCTCCTGCTTCAGAAAATGTGGAGGATGTATACGCAAATAGCCTTTCACCAATGATTTCCCCACCAATGTCTAATACTTCGTTCACTGGGTCACCTTCTAGGAGAAACAATAGACAAAAGTACTGCCTGCAGAGAAAAAACTCTAGTGGTACTGTAGGACCACTATGTTTCCAGGAATTGAACGAAGGTTTCAATGACTCCCTCATTTCACCAAAGAAAATTCGCTCGAATCCAAATGAAAACCTCAGTTCAAAAACCAAATTTATTACGCCCTTCACTCCAAAGAGTAGAGTTAGTTCAGCTACGTCCAACTCTGCTAACATTACCCCTAATAACTTAAGGTTAGATTTCAAGATTAATGTTGAAGATCAGGAAAGCGAATATTCAGAGAAACCTTTGGGCCTGGGTATTGAACTTCTTGGAAAACCAGGTCCTTCTCCTACAAAATCAGTGTCTTTAAAGAGTGCTTCTGTGGATATTATGCCTACAATTCCTGGGTCTGTAAATAATACTCCATCAGTCAATAAAGTATCCCTTTCCTCCAGTTATATAGACCAATATACACCAAGAGGTAAGCAGTTACACTTTAGCTCTATCAGTGAGAATGCATTGGGTATCAATGCTGCGACACCACATCTAAAGCCGCCGAGCCAGCAAGCACGACATCGGGAAGGCGTTTTCAATGATTTAGACCCTAATGTACTTACGAAAAATACAGACAATGAAGGAGATGATAATGAGGAAAATGAGCCTGAAAGTAGATTTGTTATTTCCGAAACGCCTTCTCCCGTTCTTAAGTCACAAAGTAAGTACGAAGGAAGATCTCCTCAATTCGGCACACACATTAAGAAAATCAACACATATACCACAAATAGTCCCTCAAAAATCACAAGAAAACTCACAACACTGCCCAGAGGTTCAATCGATAAATATGTGAAGGAAATGCCTGATAAAACATTTGAATGTTTATTTCCTGGATGTACAAAAACATTCAAGAGAAGGTATAACATCAGATCTCATATTCAAACACATTTGGAAGATCGGCCATATTCCTGTGATCACCCTGGATGTGATAAGGCATTTGTACGAAATCATGACTTGATAAGACACAAAAAATCGCACCAAGAAAAGGCGTATGCATGTCCCTGTGGTAAGAAATTCAATAGAGAAGACGCTTTGGTTGTGCACAGAAGTAGAATGATTTGCAGCGGTGGTAAAAAGTATGAAAACGTGGTAATTAAAAGGTCTCCAAGAAAAAGAGGAAGACCAAGAAAAGATGGAACTTCAAGTGTTTCTAGTAGTCCAATCAAAGAAAATATTAACAAGGATCATAATGGACAGTTGATGTTCAAGCTTGAAGATCAACTCAGAAGAGAGCGTAGTTATGATGGGAATGGAACGGGGATTATGGTTTCGCCAATGAAAACTAATCAAAGGTAA

Insert Property: Insert 1

Type Value Extra Information
Discrepancy g1540a,E514K
End on reference sequence 2130
Start on reference sequence 1

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

TTTTGATTGATTGTACAGGAAAATATACATCGCAGGGGGTTGACTTTTACCATTTCACCGCAATGGAATCAAACTTGTTGAAGAGAATGTTCACAGGCGCATACGCTACAATGACCCGATTCTTGCTAGCCTTTTCTCGGTCTTGCAAACAACCGCCGGCAGCTTAGTATATAAATACACATGTACATACCTCTCTCCGTATCCTCGTAATCATTTTCTTGTATTTATCGTCTTTTCGCTGTAAAAACTTTATCACACTTATCTCAAATACACTTATTAACCGCTTTTACTATTATCTTCTACGCTGACAGTAATATCAAACAGTGACACATATTAAACACAGTGGTTTCTTTGCATAAACACCATCAGCCTCAAGTCGTCAAGTAAAGATTTCTTGTTCATGCAGATAGATAACAATCTATATGTTGATAATTAGCGTTGCCTCATCAATGCGAGATCCGTTTAACCGGACCCTAGTGCACTTACCCCACGTTCGGTCCACTGTGTGCCGAACATGCTCCTTCACTATTTTAACATGTGGAATTAATTCTCATGTTTGACAGCTTATCATCGAACTCTAAGAGGTGATACTTATTTACTGTAAAACTGTGACGATAAAACCGGAAGGAAGAATAAGAAAACTCGAACTGATCTATAATGCCTATTTTCTGTAAAGAGTTTAAGCTATGAAAGCCTCGGCATTTTGGCCGCTCCTAGGTAGTGCTTTTTTTCCAAGGACAAAACAGTATCTTTTTCTTGAGCAGGTTTTATGTTTCGGTAATCATAAACAATAAATAAATTAATTCATTTATGTTTAAAAATAAAAAGAGCCTCGGCATTTGGCCGCTCCTAGGTAGGCTTTTTTCCAGGGCCAAACAGTTTTTTTTTCTGAGCAGGTTTATGTTCGGTAATCATAAACAATAAATAAATTATTCATTTTTTTTAAAAATAAAAAATAAAAAAGTATTTTAAATTTTTAAAAAAGTTGATTATAAGCATGTGACCTTTTGCAAGCAATTAAATTTTGCAATTTGTGATTTAGGCAAAAGTTACTATTTCTGGCTCGTGTAATATATGTATGCTAATGTGAACTTTTACAAAGTCGATATGGACTTAGTCAAAAGAAATTTTCTTAAAAATATATAGCACTAGCCAATTTAGCACTTCTTTATGAGATATATTATAGACTTTATTAAGCCAGATTTGTGTATTATATGTATTTACCCGGCGAATCATGGACATACATTCTGAAATAGGTAATATTCTCTATGGTGAGACAGCATAGATAACCTAGGATACAAGTTAAAAGCTAGTACTGTTTTGCAGTAATTTTTTTCTTTTTTATAAGAATGTTACCACCTAAATAAGTTATAAAGTCAATAGTTAAGTTTGATATTTGATTGTAAAATACCGTAATATATTTGCATGATCAAAAGGCTCAATGTTGACTAGCCAGCATGTCAACCACTATATTGATCACCGATATTAGGACTTCCACACCAACTAGTAATATGACAATAAATTCAAGATATTCTTCATGAGAATGGCCCAGCGATTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACATCGAATTAATTCCTTGAATTTTCAAAAATTCTTACTTTTTTTTTGGATGGACGCAAAGAAGTTTAATAATCATATTACATGGCATTACCACCATATACATATCCATATACATATCCATATCTAATCTTACTTATATGTTGTGGAAATGTAAAGAGCCCCATTATCTTAGCCTAAAAAAACCTTCTCTTTGGAACTTTCAGTAATACGCTTAACTGCTCATTGCTATATTGAAGTACGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAGAAAAAACCCCGGATCCTCTAGAGTCGACCATCACAAGTTTGTACAAAAAAGCAGGCTCCACCATGGATACATCAAACTCTTGGTTTGATGCCTCAAAAGTACAAAGCCTAAATTTTGACCTACAAACCAACTCTTATTACTCAAATGCTAGAGGCAGTGACCCTTCGAGTTATGCAATAGAAGGCGAATATAAAACACTAGCAACAGATGATTTAGGCAATATATTGAACCTAAACTATGGTGAAACCAACGAAGTTATAATGAATGAAATAAATGACTTAAACCTTCCGCTGGGACCACTTTCTGATGAAAAATCTGTCAAGGTTTCTACTTTCTCCGAGTTAATAGGAAATGATTGGCAAAGTATGAACTTTGACTTAGAAAATAATTCAAGAGAAGTGACTCTAAATGCAACTAGCTTGTTGAATGAAAATAGATTAAATCAAGACAGTGGTATGACTGTTTATCAAAAAACAATGAGTGATAAGCCCCACGATGAAAAGAAGATTTCCATGGCGGATAATTTATTGTCGACTATAAACAAAAGTGAAATTAATAAAGGCTTCGATAGAAACCTCGGGGAATTACTGTTACAGCAACAACAAGAGTTGCGCGAGCAATTGAGAGCACAACAAGAGGCTAATAAGAAGTTAGAGTTAGAACTCAAACAAACACAATACAAGCAACAACAACTACAGGCTACCTTAGAGAACTCTGATGGTCCACAGTTTTTATCTCCCAAAAGGAAGATCTCTCCTGCTTCAGAAAATGTGGAGGATGTATACGCAAATAGCCTTTCACCAATGATTTCCCCACCAATGTCTAATACTTCGTTCACTGGGTCACCTTCTAGGAGAAACAATAGACAAAAGTACTGCCTGCAGAGAAAAAACTCTAGTGGTACTGTAGGACCACTATGTTTCCAGGAATTGAACGAAGGTTTCAATGACTCCCTCATTTCACCAAAGAAAATTCGCTCGAATCCAAATGAAAACCTCAGTTCAAAAACCAAATTTATTACGCCCTTCACTCCAAAGAGTAGAGTTAGTTCAGCTACGTCCAACTCTGCTAACATTACCCCTAATAACTTAAGGTTAGATTTCAAGATTAATGTTGAAGATCAGGAAAGCGAATATTCAGAGAAACCTTTGGGCCTGGGTATTGAACTTCTTGGAAAACCAGGTCCTTCTCCTACAAAATCAGTGTCTTTAAAGAGTGCTTCTGTGGATATTATGCCTACAATTCCTGGGTCTGTAAATAATACTCCATCAGTCAATAAAGTATCCCTTTCCTCCAGTTATATAGACCAATATACACCAAGAGGTAAGCAGTTACACTTTAGCTCTATCAGTGAGAATGCATTGGGTATCAATGCTGCGACACCACATCTAAAGCCGCCGAGCCAGCAAGCACGACATCGGGAAGGCGTTTTCAATGATTTAGACCCTAATGTACTTACGAAAAATACAGACAATGAAGGAGATGATAATGAGGAAAATGAGCCTGAAAGTAGATTTGTTATTTCCGAAACGCCTTCTCCCGTTCTTAAGTCACAAAGTAAGTACGAAGGAAGATCTCCTCAATTCGGCACACACATTAAGAAAATCAACACATATACCACAAATAGTCCCTCAAAAATCACAAGAAAACTCACAACACTGCCCAGAGGTTCAATCGATAAATATGTGAAGGAAATGCCTGATAAAACATTTGAATGTTTATTTCCTGGATGTACAAAAACATTCAAGAGAAGGTATAACATCAGATCTCATATTCAAACACATTTGGAAGATCGGCCATATTCCTGTGATCACCCTGGATGTGATAAGGCATTTGTACGAAATCATGACTTGATAAGACACAAAAAATCGCACCAAGAAAAGGCGTATGCATGTCCCTGTGGTAAGAAATTCAATAGAGAAGACGCTTTGGTTGTGCACAGAAGTAGAATGATTTGCAGCGGTGGTAAAAAGTATGAAAACGTGGTAATTAAAAGGTCTCCAAGAAAAAGAGGAAGACCAAGAAAAGATGGAACTTCAAGTGTTTCTAGTAGTCCAATCAAAGAAAATATTAACAAGGATCATAATGGACAGTTGATGTTCAAGCTTGAAGATCAACTCAGAAGAGAGCGTAGTTATGATGGGAATGGAACGGGGATTATGGTTTCGCCAATGAAAACTAATCAAAGGTAAGACCCAGCTTTCTTGTACAAAGTGGTGATGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGGGGGGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAATTAGCTTTTCAATTCATCATTTTTTTTTTATTCTTTTTTTTGATTTCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACATGCAGGAAACGAAGATAAATCATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAAAAAACTGTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAGAGCTTCAATTTAATTATATCAGTTATTACCCAATTCTCATGTTTGACAGCTTATCATCGATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGTGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAGAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAAGGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAGGTTATTACTGAGTAGTATTTATTTAAGTATTGTTTGTGCACTTGCCTGCAAGCCTTTTGAAAAGCAAGCATAAAAGATCTAAACATAAAATCTGTAAAATAACAAGATGTAAAGATAATGCTAAATCATTTGGCT

Vector Information:

Vector Name:pBY011
Type:bacterial plasmid
Description:Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Properties:Gateway, acceptor (destination), inducible expression, yeast expression, yeast shuttle
Comments:This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Map:pBY011_map.pdf
Sequence:pBY011.txt
Size (bp):7920
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
yeast uracil

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.