Clone: ScCD00097978

Clone ID:ScCD00097978
Is Verified:N
Status:AVAILABLE
Source:HIP
Description:
Comments:
Type:cDNA
Verification Method:Insert was fully sequenced in parent vector
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

HIP Clone ID 261203
HIP Master Clone ID 561
Original Clone ID FLH261203.01X

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 1311 Saccharomyces cerevisiae CLOSED Genomic DNA template YBR236C ABD1 Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
Insert1
Size (bp)1311
SpeciesSaccharomyces cerevisiae
FormatCLOSED
Tissue SourceGenomic DNA template
Species Specific ID YBR236C
Gene SymbolABD1
Gene NameMethyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
Keyword

Insert Sequence:

ATGTCAACCAAACCAGAGAAGCCAATATGGATGTCACAAGAAGACTATGACCGTCAGTACGGCTCAATTACTGGTGATGAATCGTCCACAGTCTCAAAAAAAGACTCCAAAGTTACTGCCAATGCGCCTGGTGATGGTAATGGATCATTACCAGTTTTGCAAAGCAGCTCTATCCTTACTTCTAAGGTGTCTGATCTACCCATTGAGGCAGAATCCGGGTTTAAAATCCAAAAGAGAAGACATGAAAGGTATGATCAAGAGGAAAGATTACGCAAACAGCGTGCTCAAAAATTGAGAGAAGAGCAACTAAAGAGACATGAAATAGAGATGACTGCAAATAGATCCATAAATGTTGACCAGATTGTCCGAGAGCACTGTAATGAACGTACAATAATCGCCAATCGCGCTAAGAGGAATCTATCGCCTATTATTAAGCTTCGTAATTTTAACAATGCGATTAAGTATATGTTAATTGACAAATATACAAAACCTGGAGATGTCGTTTTAGAACTCGGATGTGGCAAAGGTGGTGATTTAAGAAAATATGGTGCTGCAGGTATTTCACAGTTTATTGGTATTGACATTTCCAATGCCTCTATCCAAGAAGCTCATAAGAGATACCGGTCCATGAGAAATTTGGACTACCAAGTTGTGCTAATTACTGGGGATTGCTTTGGCGAATCATTAGGTGTTGCAGTAGAGCCCTTCCCAGACTGTAGATTTCCGTGTGATATCGTTTCGACGCAATTTTGCTTGCATTATGCTTTTGAGACCGAAGAAAAAGCAAGGAGAGCTTTACTGAATGTCGCCAAATCACTCAAAATTGGTGGTCACTTTTTTGGCACGATACCAGATTCAGAATTCATTCGTTATAAACTGAATAAATTTCCCAAAGAAGTGGAAAAGCCATCTTGGGGAAACTCCATTTATAAAGTGACATTTGAGAATAATTCATACCAAAAGAATGATTATGAGTTCACATCGCCTTATGGTCAAATGTATACTTATTGGCTAGAAGATGCTATAGATAATGTTCCTGAATATGTTGTGCCATTCGAAACCTTAAGAAGTTTGGCTGATGAATACGGTTTGGAACTAGTATCGCAAATGCCTTTTAATAAATTTTTTGTCCAAGAAATTCCCAAATGGATTGAGAGGTTTTCGCCTAAAATGAGGGAAGGTTTACAAAGATCTGATGGTAGATATGGTGTTGAAGGGGATGAAAAGGAAGCGGCATCATATTTCTATACCATGTTTGCATTCCGTAAGGTAAAACAGTATATCGAACCGGAAAGCGTAAAGCCCAACTGA

Insert Property: Insert 1

Type Value Extra Information
Discrepancy a377g,Y126C
End on reference sequence 1311
Start on reference sequence 1

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

TTTTGATTGATTGTACAGGAAAATATACATCGCAGGGGGTTGACTTTTACCATTTCACCGCAATGGAATCAAACTTGTTGAAGAGAATGTTCACAGGCGCATACGCTACAATGACCCGATTCTTGCTAGCCTTTTCTCGGTCTTGCAAACAACCGCCGGCAGCTTAGTATATAAATACACATGTACATACCTCTCTCCGTATCCTCGTAATCATTTTCTTGTATTTATCGTCTTTTCGCTGTAAAAACTTTATCACACTTATCTCAAATACACTTATTAACCGCTTTTACTATTATCTTCTACGCTGACAGTAATATCAAACAGTGACACATATTAAACACAGTGGTTTCTTTGCATAAACACCATCAGCCTCAAGTCGTCAAGTAAAGATTTCTTGTTCATGCAGATAGATAACAATCTATATGTTGATAATTAGCGTTGCCTCATCAATGCGAGATCCGTTTAACCGGACCCTAGTGCACTTACCCCACGTTCGGTCCACTGTGTGCCGAACATGCTCCTTCACTATTTTAACATGTGGAATTAATTCTCATGTTTGACAGCTTATCATCGAACTCTAAGAGGTGATACTTATTTACTGTAAAACTGTGACGATAAAACCGGAAGGAAGAATAAGAAAACTCGAACTGATCTATAATGCCTATTTTCTGTAAAGAGTTTAAGCTATGAAAGCCTCGGCATTTTGGCCGCTCCTAGGTAGTGCTTTTTTTCCAAGGACAAAACAGTATCTTTTTCTTGAGCAGGTTTTATGTTTCGGTAATCATAAACAATAAATAAATTAATTCATTTATGTTTAAAAATAAAAAGAGCCTCGGCATTTGGCCGCTCCTAGGTAGGCTTTTTTCCAGGGCCAAACAGTTTTTTTTTCTGAGCAGGTTTATGTTCGGTAATCATAAACAATAAATAAATTATTCATTTTTTTTAAAAATAAAAAATAAAAAAGTATTTTAAATTTTTAAAAAAGTTGATTATAAGCATGTGACCTTTTGCAAGCAATTAAATTTTGCAATTTGTGATTTAGGCAAAAGTTACTATTTCTGGCTCGTGTAATATATGTATGCTAATGTGAACTTTTACAAAGTCGATATGGACTTAGTCAAAAGAAATTTTCTTAAAAATATATAGCACTAGCCAATTTAGCACTTCTTTATGAGATATATTATAGACTTTATTAAGCCAGATTTGTGTATTATATGTATTTACCCGGCGAATCATGGACATACATTCTGAAATAGGTAATATTCTCTATGGTGAGACAGCATAGATAACCTAGGATACAAGTTAAAAGCTAGTACTGTTTTGCAGTAATTTTTTTCTTTTTTATAAGAATGTTACCACCTAAATAAGTTATAAAGTCAATAGTTAAGTTTGATATTTGATTGTAAAATACCGTAATATATTTGCATGATCAAAAGGCTCAATGTTGACTAGCCAGCATGTCAACCACTATATTGATCACCGATATTAGGACTTCCACACCAACTAGTAATATGACAATAAATTCAAGATATTCTTCATGAGAATGGCCCAGCGATTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACATCGAATTAATTCCTTGAATTTTCAAAAATTCTTACTTTTTTTTTGGATGGACGCAAAGAAGTTTAATAATCATATTACATGGCATTACCACCATATACATATCCATATACATATCCATATCTAATCTTACTTATATGTTGTGGAAATGTAAAGAGCCCCATTATCTTAGCCTAAAAAAACCTTCTCTTTGGAACTTTCAGTAATACGCTTAACTGCTCATTGCTATATTGAAGTACGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAGAAAAAACCCCGGATCCTCTAGAGTCGACCATCACAAGTTTGTACAAAAAAGCAGGCTCCACCATGTCAACCAAACCAGAGAAGCCAATATGGATGTCACAAGAAGACTATGACCGTCAGTACGGCTCAATTACTGGTGATGAATCGTCCACAGTCTCAAAAAAAGACTCCAAAGTTACTGCCAATGCGCCTGGTGATGGTAATGGATCATTACCAGTTTTGCAAAGCAGCTCTATCCTTACTTCTAAGGTGTCTGATCTACCCATTGAGGCAGAATCCGGGTTTAAAATCCAAAAGAGAAGACATGAAAGGTATGATCAAGAGGAAAGATTACGCAAACAGCGTGCTCAAAAATTGAGAGAAGAGCAACTAAAGAGACATGAAATAGAGATGACTGCAAATAGATCCATAAATGTTGACCAGATTGTCCGAGAGCACTGTAATGAACGTACAATAATCGCCAATCGCGCTAAGAGGAATCTATCGCCTATTATTAAGCTTCGTAATTTTAACAATGCGATTAAGTATATGTTAATTGACAAATATACAAAACCTGGAGATGTCGTTTTAGAACTCGGATGTGGCAAAGGTGGTGATTTAAGAAAATATGGTGCTGCAGGTATTTCACAGTTTATTGGTATTGACATTTCCAATGCCTCTATCCAAGAAGCTCATAAGAGATACCGGTCCATGAGAAATTTGGACTACCAAGTTGTGCTAATTACTGGGGATTGCTTTGGCGAATCATTAGGTGTTGCAGTAGAGCCCTTCCCAGACTGTAGATTTCCGTGTGATATCGTTTCGACGCAATTTTGCTTGCATTATGCTTTTGAGACCGAAGAAAAAGCAAGGAGAGCTTTACTGAATGTCGCCAAATCACTCAAAATTGGTGGTCACTTTTTTGGCACGATACCAGATTCAGAATTCATTCGTTATAAACTGAATAAATTTCCCAAAGAAGTGGAAAAGCCATCTTGGGGAAACTCCATTTATAAAGTGACATTTGAGAATAATTCATACCAAAAGAATGATTATGAGTTCACATCGCCTTATGGTCAAATGTATACTTATTGGCTAGAAGATGCTATAGATAATGTTCCTGAATATGTTGTGCCATTCGAAACCTTAAGAAGTTTGGCTGATGAATACGGTTTGGAACTAGTATCGCAAATGCCTTTTAATAAATTTTTTGTCCAAGAAATTCCCAAATGGATTGAGAGGTTTTCGCCTAAAATGAGGGAAGGTTTACAAAGATCTGATGGTAGATATGGTGTTGAAGGGGATGAAAAGGAAGCGGCATCATATTTCTATACCATGTTTGCATTCCGTAAGGTAAAACAGTATATCGAACCGGAAAGCGTAAAGCCCAACTGAGACCCAGCTTTCTTGTACAAAGTGGTGATGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGGGGGGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAATTAGCTTTTCAATTCATCATTTTTTTTTTATTCTTTTTTTTGATTTCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACATGCAGGAAACGAAGATAAATCATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAAAAAACTGTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAGAGCTTCAATTTAATTATATCAGTTATTACCCAATTCTCATGTTTGACAGCTTATCATCGATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGTGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAGAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAAGGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAGGTTATTACTGAGTAGTATTTATTTAAGTATTGTTTGTGCACTTGCCTGCAAGCCTTTTGAAAAGCAAGCATAAAAGATCTAAACATAAAATCTGTAAAATAACAAGATGTAAAGATAATGCTAAATCATTTGGCT

Vector Information:

Vector Name:pBY011
Type:bacterial plasmid
Description:Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Properties:Gateway, acceptor (destination), inducible expression, yeast expression, yeast shuttle
Comments:This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Map:pBY011_map.pdf
Sequence:pBY011.txt
Size (bp):7920
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
yeast uracil

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.