Clone: ScCD00009040

Clone ID:ScCD00009040
Is Verified:Y
Status:AVAILABLE
Source:HIP
Description:
Comments:
Type:cDNA
Verification Method:Sequence Verification
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

Original Clone ID FLH149032.01X
HIP Clone ID 149032
HIP Master Clone ID 125351

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 1536 Saccharomyces cerevisiae CLOSED Genomic DNA template YOR310C NOP58 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
Insert1
Size (bp)1536
SpeciesSaccharomyces cerevisiae
FormatCLOSED
Tissue SourceGenomic DNA template
Species Specific ID YOR310C
Gene SymbolNOP58
Gene NameProtein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
Keyword

Insert Sequence:

ATGGCTTACGTTTTAACTGAAACTTCAGCTGGTTATGCTCTTTTAAAAGCTTCCGATAAGAAAATCTACAAATCTTCAAGCTTAATTCAAGACTTGGATAGCTCTGACAAAGTCTTGAAGGAATTCAAGATCGCCGCCTTTTCCAAGTTCAACTCTGCAGCTAACGCTTTAGAGGAAGCCAACTCCATTATTGAAGGTAAAGTTTCTTCCCAATTAGAAAAACTTCTGGAAGAAATTAAAAAGGACAAGAAGTCGACCTTGATTGTTAGTGAAACTAAACTGGCAAACGCTATTAATAAGTTGGGATTGAACTTCAATGTTGTTTCCGATGCTGTAACCTTAGATATCTATAGAGCTATCAAAGAATATTTGCCAGAATTGCTGCCAGGTATGTCTGATAATGATCTAAGCAAGATGTCTTTGGGTTTAGCTCATTCCATCGGTCGTCACAAATTAAAATTTTCTGCTGACAAAGTTGATGTTATGATTATTCAAGCAATTGCTTTATTGGATGATTTAGATAAAGAATTAAACACATATGCCATGAGATGTAAAGAATGGTACGGGTGGCACTTTCCTGAGTTAGCTAAGATTGTTACTGATTCCGTCGCTTATGCGAGAATTATTCTAACAATGGGTATCAGATCCAAGGCTTCTGAAACAGATTTGAGTGAAATCTTACCAGAAGAGATTGAAGAACGTGTCAAAACCGCTGCTGAAGTGTCTATGGGTACTGAAATCACTCAAACCGATCTAGACAACATTAATGCTTTAGCTGAACAAATAGTCGAATTTGCCGCTTACAGAGAACAGTTGTCCAATTACTTGTCTGCAAGAATGAAGGCTATTGCTCCAAACTTGACTCAGTTGGTTGGTGAATTAGTTGGTGCTAGACTTATCGCTCATTCAGGTTCCTTAATTTCCTTGGCCAAATCACCAGCTTCTACCATCCAAATATTAGGTGCCGAAAAGGCTTTATTCAGAGCTTTAAAGACCAAGCATGATACACCAAAGTATGGTTTGCTATATCATGCCTCTCTTGTTGGTCAAGCTACTGGTAAGAACAAAGGTAAGATTGCAAGAGTTCTGGCTGCTAAGGCCGCTGTCTCATTGCGTTATGATGCTTTAGCTGAAGATAGAGACGATTCAGGTGACATTGGTCTAGAATCAAGAGCCAAAGTAGAAAATAGATTATCCCAATTAGAAGGTAGAGATTTAAGAACTACTCCAAAGGTTGTTCGTGAGGCTAAGAAGGTGGAAATGACTGAAGCTAGGGCCTACAATGCAGATGCTGATACCGCAAAGGCTGCTTCTGATTCTGAATCCGATTCTGATGATGAAGAGGAAGAAAAGAAAGAAAAGAAAGAAAAGAAGAGAAAGAGAGACGATGATGAAGATTCCAAGGATTCTAAAAAAGCTAAGAAGGAAAAGAAGGACAAGAAGGAAAAGAAGGAAAAGAAGGAAAAGAAGGAGAAGAAAGAGAAGAAAGAGAAGAAGGAAAAGAAGTCCAAGAAAGAGAAGAAAGAGAAGAAATAG

Insert Property: Insert 1

Type Value Extra Information
End on reference sequence 1536
Start on reference sequence 1

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTATGGCTTACGTTTTAACTGAAACTTCAGCTGGTTATGCTCTTTTAAAAGCTTCCGATAAGAAAATCTACAAATCTTCAAGCTTAATTCAAGACTTGGATAGCTCTGACAAAGTCTTGAAGGAATTCAAGATCGCCGCCTTTTCCAAGTTCAACTCTGCAGCTAACGCTTTAGAGGAAGCCAACTCCATTATTGAAGGTAAAGTTTCTTCCCAATTAGAAAAACTTCTGGAAGAAATTAAAAAGGACAAGAAGTCGACCTTGATTGTTAGTGAAACTAAACTGGCAAACGCTATTAATAAGTTGGGATTGAACTTCAATGTTGTTTCCGATGCTGTAACCTTAGATATCTATAGAGCTATCAAAGAATATTTGCCAGAATTGCTGCCAGGTATGTCTGATAATGATCTAAGCAAGATGTCTTTGGGTTTAGCTCATTCCATCGGTCGTCACAAATTAAAATTTTCTGCTGACAAAGTTGATGTTATGATTATTCAAGCAATTGCTTTATTGGATGATTTAGATAAAGAATTAAACACATATGCCATGAGATGTAAAGAATGGTACGGGTGGCACTTTCCTGAGTTAGCTAAGATTGTTACTGATTCCGTCGCTTATGCGAGAATTATTCTAACAATGGGTATCAGATCCAAGGCTTCTGAAACAGATTTGAGTGAAATCTTACCAGAAGAGATTGAAGAACGTGTCAAAACCGCTGCTGAAGTGTCTATGGGTACTGAAATCACTCAAACCGATCTAGACAACATTAATGCTTTAGCTGAACAAATAGTCGAATTTGCCGCTTACAGAGAACAGTTGTCCAATTACTTGTCTGCAAGAATGAAGGCTATTGCTCCAAACTTGACTCAGTTGGTTGGTGAATTAGTTGGTGCTAGACTTATCGCTCATTCAGGTTCCTTAATTTCCTTGGCCAAATCACCAGCTTCTACCATCCAAATATTAGGTGCCGAAAAGGCTTTATTCAGAGCTTTAAAGACCAAGCATGATACACCAAAGTATGGTTTGCTATATCATGCCTCTCTTGTTGGTCAAGCTACTGGTAAGAACAAAGGTAAGATTGCAAGAGTTCTGGCTGCTAAGGCCGCTGTCTCATTGCGTTATGATGCTTTAGCTGAAGATAGAGACGATTCAGGTGACATTGGTCTAGAATCAAGAGCCAAAGTAGAAAATAGATTATCCCAATTAGAAGGTAGAGATTTAAGAACTACTCCAAAGGTTGTTCGTGAGGCTAAGAAGGTGGAAATGACTGAAGCTAGGGCCTACAATGCAGATGCTGATACCGCAAAGGCTGCTTCTGATTCTGAATCCGATTCTGATGATGAAGAGGAAGAAAAGAAAGAAAAGAAAGAAAAGAAGAGAAAGAGAGACGATGATGAAGATTCCAAGGATTCTAAAAAAGCTAAGAAGGAAAAGAAGGACAAGAAGGAAAAGAAGGAAAAGAAGGAAAAGAAGGAGAAGAAAGAGAAGAAAGAGAAGAAGGAAAAGAAGTCCAAGAAAGAGAAGAAAGAGAAGAAATAGAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Information:

Vector Name:pDONR221
Type:bacterial plasmid
Description:Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Properties:Gateway, donor (entry), recombinational cloning
Comments:The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Map:pdonr221_pdonrzeo_map.pdf
Sequence:pDONR221_FASTA.txt
Size (bp):4762
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial kanamycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 50ug/mL kanamycin Growth with the single antibiotic in LB at 37 degrees is recommended. Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Authors:

Author Name Author Type
HIP Academic Institute

Publications:

PMID Title
17322287 Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.