Clone: EvNO00079527 ← Click Here To Order

Clone ID:EvNO00079527
Is Verified:U
Status:AVAILABLE
Source:HIP
Description:Empty vector that adds EGFP fusion to insert (discontinued by supplier); kan resistance; Creator (Cre/Lox-type) recombinational cloning.
Comments:Vector discontinued by original supplier; helpful as control for with-insert constructs in the repository collection.
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:
Map:

Related Identifiers:

Original Clone ID pLPS-3'EGFP
SYNONYM pLPS-3pEGFP

Sequencing Information:

Phred Score: 843
Date: 2016-02-16

Last Passing Sequencing Read:

TCAGATCCGCTAGCATAACTTCGTATAGCATACATTATACGAAGTTATAGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTGGATCTCGAGCTCAAGCTTCGAATTCAGGGTTTCCTTGACAATATCATACTTATCCTGTCCCTTTTTTTTCCACAGCTACCGGTCGCGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGC

Vector Information:

Vector Name:pLPS-3'EGFP
Type:bacterial plasmid
Description:Mammalian expression vector with CMV promoter that adds C-terminal fluorescent (EGFP) tag; kanamycin resistance (neomycin for mammalian expression); recombinational cloning.
Properties:Creator, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning, ssDNA production, with tag/fusion/marker
Comments:Clontech vector that has since been discontinued
Map:pLPS3pEGFP.pdf
Sequence:pLPS3pEGFP.txt
Size (bp):4900
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
Phage-resistant DH5-alpha Y From HIP lab

Antibiotic Selections:

Host Type Marker
bacterial kanamycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 50ug/mL kanamycin Growth with the single antibiotic in LB at 37 degrees is recommended. Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Authors:

Author Name Author Type
BD Biosciences Clontech Adademic researcher, clone donor and PI