Clone: EvNO00061638 ← Click Here To Order

Clone ID:EvNO00061638
Is Verified:U
Status:AVAILABLE
Source:Lee lab (HGTI/HMS)
Description:Lentiviral vector that expresses nuclear localization signal (nls)-containing Cre recombinase from internal CMV promoter; amp resistance; restriction enzyme cloning.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pHRST-nCre
SYNONYM pHRST-nlsCre

Vector Information:

Vector Name:pHRST-nCre
Type:bacterial plasmid
Description:Lentiviral vector that expresses nuclear localization signal (nls)-containing Cre recombinase from internal CMV promoter; ampicillin resistance; restriction enzyme cloning.
Properties:lentiviral infection, mammalian expression, mammalian transduction, retroviral vector
Comments:
Map:pHRST-nCre.pdf
Sequence:pHRST-nCre.txt
Size (bp):11028
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha N E. coli host strain DH5-alpha (is recommended by clone donor)
EB5-alpha Y T1, T5 phage-resistant E. coli host strain EB5-alpha (DH5-alpha-like strain) from EdgeBiosystems

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Jeng-Shin Lee Academic researcher, clone donor and PI