Related Identifiers:
Original Clone ID |
pHRST-nCre |
SYNONYM |
pHRST-nlsCre |
Vector Information:
Vector Name: | pHRST-nCre |
Type: | bacterial plasmid |
Description: | Lentiviral vector that expresses nuclear localization signal (nls)-containing Cre recombinase from internal CMV promoter; ampicillin resistance; restriction enzyme cloning. |
Properties: | lentiviral infection, mammalian expression, mammalian transduction, retroviral vector |
Comments: | |
Map: | pHRST-nCre.pdf |
Sequence: | pHRST-nCre.txt |
Size (bp): | 11028 |
Form: | dsDNA |
Host Information:
Host Strain |
Is Used In Distribution |
Description |
DH5-alpha |
N |
E. coli host strain DH5-alpha (is recommended by clone donor) |
EB5-alpha |
Y |
T1, T5 phage-resistant E. coli host strain EB5-alpha (DH5-alpha-like strain) from EdgeBiosystems |
Antibiotic Selections:
Host Type |
Marker |
bacterial |
ampicillin |
Recommended Growth Condition:
Host Type |
Selection Condition |
Growth Condition |
Comments |
bacterial |
100 ug/mL ampicillin |
Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
Author Name |
Author Type |
Jeng-Shin Lee |
Academic researcher, clone donor and PI |