Clone: EvNO00061635 ← Click Here To Order

Clone ID:EvNO00061635
Is Verified:U
Status:AVAILABLE
Source:Lee lab (HGTI/HMS)
Description:Lentiviral vector that expresses insert from internal PGK promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; amp resistance; restriction enzyme cloning.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pHAGE-PGK-MCS-IRES-ZsGreen-W

Sequencing Information:

Phred Score: 204
Date: 2015-03-18

Last Passing Sequencing Read:

CGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCCACACATATGGCCCAGTCCAAGCACGGCCTGACCA

Vector Information:

Vector Name:pHAGE-PGK-MCS-IRES-ZsGreen-W
Type:bacterial plasmid
Description:Lentiviral vector that expresses insert from internal PGK promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; ampicillin resistance; restriction enzyme cloning.
Properties:fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Comments:ZsGreen described as like eGFP but brighter
Map:pHAGE-PGK-MCS-IZsGreen-W.pdf
Sequence:pHAGE-PGK-MCS-IZsGreen-W.txt
Size (bp):7622
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha N E. coli host strain DH5-alpha (is recommended by clone donor)
EB5-alpha Y T1, T5 phage-resistant E. coli host strain EB5-alpha (DH5-alpha-like strain) from EdgeBiosystems

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Jeng-Shin Lee Academic researcher, clone donor and PI