Clone: EvNO00061634 ← Click Here To Order

Clone ID:EvNO00061634
Is Verified:U
Status:AVAILABLE
Source:Lee lab (HGTI/HMS)
Description:Lentiviral vector that expresses eGFP fluorescent marker from CMV promoter, with WPRE; amp resistance; restriction enzyme cloning.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pHAGE-CMV-eGFP-W

Sequencing Information:

Phred Score: 933
Date: 2017-04-26

Last Passing Sequencing Read:

>27126-33_EvNO00061634-CMV-F_A5 933 1442 36 974 NNNNNTTCGTTGAGCATTACAACTCGTTAGTGACCGTCAGATCGCCTGGA GACCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGCGGCCGCCAT GGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCG AGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGC GAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCAC CGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACG GCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTC TTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTT CAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCG ACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGAC GGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGT CTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGA TCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAG CAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTA CCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATC ACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATG GACGAGCTGTACAAGTAAGGATCCTAATCAACCTCTGGATTACAAAATTT GTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGT GGATACGCTGCTTTAAATGCCTTGTATCATGCTATTGCTTCCCGTATGGC TTTCATTTTCTCCTCCTTGTATAAATCCTGGGTGCTGTCTCTTTATGNAA GAGTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCT GACCGCACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTC CGGGACTTTCGCTTTCCCCTCCCNTATGCACGGCGAACTCATCCCGCCTG CCTTGCCGCCTGCTGACAGGGCTCGCTGTTGGCACTGACAATCCTGGGGT GTCGGGAAACATCGTCCTTCCTGCCGCTCCCTGTGTGCCCCTNATCTGCG GGGAGTCTTTTGATAGTCCTTGGCTAATCAAGGACTTTCTCGCGGCTGTG CGGTTTGGGGCTTTCCGGTTCCTTCTCTTAAAAAGGAATCCTTGGCCCCC TCCCCGATCTTAAAAAATAAAGGGTGTAATTCCTTTTAAAAGGGCTGAGG

Vector Information:

Vector Name:pHAGE-CMV-eGFP-W
Type:bacterial plasmid
Description:Lentiviral vector that expresses eGFP fluorescent marker from CMV promoter, with WPRE; ampicillin resistance; restriction enzyme cloning.
Properties:fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Comments:
Map:pHAGE-CMV-eGFP-W.pdf
Sequence:pHAGE-CMV-eGFP-W.txt
Size (bp):7149
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha N E. coli host strain DH5-alpha (is recommended by clone donor)
EB5-alpha Y T1, T5 phage-resistant E. coli host strain EB5-alpha (DH5-alpha-like strain) from EdgeBiosystems

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Jeng-Shin Lee Academic researcher, clone donor and PI