Clone: EvNO00061628 ← Click Here To Order

Clone ID:EvNO00061628
Is Verified:U
Status:AVAILABLE
Source:Mekalanos lab
Description:Mariner transposon fragment-containing vector used to create pFD1; chloramphenicol, kanamycin and tetracycline resistance; restriction enzyme cloning.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:
Map:

Related Identifiers:

SYNONYM pMEnt in XL1Blue
Original Clone ID pMEnt
SYNONYM Mekalanos Strain C36

Sequencing Information:

Phred Score: 0
Date: 2012-07-23

Last Passing Sequencing Read:

pass

Vector Information:

Vector Name:pMEnt
Type:bacterial plasmid
Description:Mariner transposon fragment-containing vector used to create pFD1; chloramphenicol, kanamycin and tetracycline resistance; restriction enzyme cloning.
Properties:mutagenesis vector, transposon mediated gene disruption
Comments:See also pFD1
Map:
Sequence:
Size (bp):0
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
XL1 Blue Y E. coli host strain XL1 Blue (Mekalanos lab)

Antibiotic Selections:

Host Type Marker
bacterial chloramphenicol
bacterial kanamycin
bacterial tetracycline

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 30 ug/mL chloramphenicol Growth at 37 degrees C in LB supplemented with chloramphenicol (30 ug/mL)

Authors:

Author Name Author Type
John Mekalanos Academic researcher, clone PI
Su Chiang Academic researcher, clone donor

Publications:

PMID Title
9990078 In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria