Clone: EvNO00061626
Clone ID: | EvNO00061626 |
Is Verified: | U |
Status: | AVAILABLE |
Source: | Mekalanos lab |
Description: | Vector for bacterial in vivo expression technology (IVET) transposon insert-based approach to identification of promoters that drive expression of promotorless marker in the vector; amp resistance; restriction enzyme cloning. |
Comments: | |
Type: | No insert |
Verification Method: | |
Distribution: | Academic and non-profit labs |
Special MTA: |
Related Identifiers:
SYNONYM | pIVET8 in DH5-alpha lambda-pir |
Original Clone ID | pIVET8 |
SYNONYM | Mekalanos Strain C5 |
Sequencing Information:
Phred Score: | 835 |
Date: | 2018-03-28 |
Last Passing Sequencing Read:
NNAACTTCTTTAGATAACAAT::GCTGAAAAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCCTTTTTGTCCGGTGTTGGGTTGAAGGTGAAGCCGGTCGGGGCCGCAGCGGGGGCCGGCTTTTCAGCCTTGCCCCCCTGCTTCGGCCGCCGTGGCTCCGGCGTCTTGGGTGCCGGCGCGGGTTCCGCAGCCTTGGCCTGCGGTGCGGGCACATCGGCGGGCTTGGCCTTGATGTGCCGCCTGGCGTGCGAGCGGAACGTCTCGTAGGAGAACTTGACCTTCCCCGTTTCCCGCATGTGCTCCCAAATGGTGACGAGCGCATAGCCGGACGCTAACGCCGCCTCGACATCCGCCCTCACCGCCAGGAACGCAACCGCAGCCTCATCACGCCGGCGCTTCTTGGCCGCGCGGGATTCAACCCACTCGGCCAGCTCGTCGGTGTAGCTCTTTGGCATCGTCTCTCGCCT:GTCCCCCTCGTTCAGTAATTTCCTGCATTTGCCTGTTTCCAATCGGTAGATATTCCACAAAACAGCAGGGAAGCAGCGCTTTTTCGCTGCATACCCCTGCTTCCGGGTCATTAAAACCAATTTTTTCGGTAATACCATTCCTTTTTCGACGAAAATACAGGATTT:GCCAAAGGGGTCCCGGAAAAATTTCCTTGGGGAACCCACAGGGCTCCCCCCGGCAAGAATAAGTAAATAAGGCCCCCCGGGAAAGGGGGGATCCTTCCTCCTGTGCCCCTTATCCCCCCCGGGGGGGCCCCACGAGAAACCGGCTTTTTAGAGCTTGGCCGTTTCTCCCGCGTTAAACATAGAAGCCAAAGAATAGTCTTATAAACATCTCCAACCAGAGAGGGGCCCCCCTCTCTAGAGTTATCCCTTTCAATAAACAAACAAA
Vector Information:
Vector Name: | pIVET8 |
Type: | bacterial plasmid |
Description: | Vector for bacterial in vivo expression technology (IVET) transposon insert-based approach to identification of promoters that drive expression of promotorless marker in the vector; amp resistance; restriction enzyme cloning. |
Properties: | bacterial expression, promoter trap, transposon mediated gene disruption |
Comments: | |
Map: | |
Sequence: | |
Size (bp): | 0 |
Form: | dsDNA |
Host Information:
Host Strain | Is Used In Distribution | Description |
DH5-alpha lambda-pir | Y | E. coli host strain DH5-alpha lambda-pir |
Antibiotic Selections:
Host Type | Marker |
bacterial | ampicillin |
Recommended Growth Condition:
Host Type | Selection Condition | Growth Condition | Comments |
bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
Author Name | Author Type |
John Mekalanos | Academic researcher, clone PI |
Su Chiang | Academic researcher, clone donor |
Publications:
PMID | Title |
7846034 | Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host |
9023360 | Bacterial infection as assessed by in vivo gene expression |