Clone: EvNO00061626
| Clone ID: | EvNO00061626 |
| Is Verified: | U |
| Status: | AVAILABLE |
| Source: | Mekalanos lab |
| Description: | Vector for bacterial in vivo expression technology (IVET) transposon insert-based approach to identification of promoters that drive expression of promotorless marker in the vector; amp resistance; restriction enzyme cloning. |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
Related Identifiers:
| SYNONYM | pIVET8 in DH5-alpha lambda-pir |
| Original Clone ID | pIVET8 |
| SYNONYM | Mekalanos Strain C5 |
Sequencing Information:
| Phred Score: | 835 |
| Date: | 2018-03-28 |
Last Passing Sequencing Read:
NNAACTTCTTTAGATAACAAT::GCTGAAAAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCCTTTTTGTCCGGTGTTGGGTTGAAGGTGAAGCCGGTCGGGGCCGCAGCGGGGGCCGGCTTTTCAGCCTTGCCCCCCTGCTTCGGCCGCCGTGGCTCCGGCGTCTTGGGTGCCGGCGCGGGTTCCGCAGCCTTGGCCTGCGGTGCGGGCACATCGGCGGGCTTGGCCTTGATGTGCCGCCTGGCGTGCGAGCGGAACGTCTCGTAGGAGAACTTGACCTTCCCCGTTTCCCGCATGTGCTCCCAAATGGTGACGAGCGCATAGCCGGACGCTAACGCCGCCTCGACATCCGCCCTCACCGCCAGGAACGCAACCGCAGCCTCATCACGCCGGCGCTTCTTGGCCGCGCGGGATTCAACCCACTCGGCCAGCTCGTCGGTGTAGCTCTTTGGCATCGTCTCTCGCCT:GTCCCCCTCGTTCAGTAATTTCCTGCATTTGCCTGTTTCCAATCGGTAGATATTCCACAAAACAGCAGGGAAGCAGCGCTTTTTCGCTGCATACCCCTGCTTCCGGGTCATTAAAACCAATTTTTTCGGTAATACCATTCCTTTTTCGACGAAAATACAGGATTT:GCCAAAGGGGTCCCGGAAAAATTTCCTTGGGGAACCCACAGGGCTCCCCCCGGCAAGAATAAGTAAATAAGGCCCCCCGGGAAAGGGGGGATCCTTCCTCCTGTGCCCCTTATCCCCCCCGGGGGGGCCCCACGAGAAACCGGCTTTTTAGAGCTTGGCCGTTTCTCCCGCGTTAAACATAGAAGCCAAAGAATAGTCTTATAAACATCTCCAACCAGAGAGGGGCCCCCCTCTCTAGAGTTATCCCTTTCAATAAACAAACAAA
Vector Information:
| Vector Name: | pIVET8 |
| Type: | bacterial plasmid |
| Description: | Vector for bacterial in vivo expression technology (IVET) transposon insert-based approach to identification of promoters that drive expression of promotorless marker in the vector; amp resistance; restriction enzyme cloning. |
| Properties: | bacterial expression, promoter trap, transposon mediated gene disruption |
| Comments: | |
| Map: | |
| Sequence: | |
| Size (bp): | 0 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| DH5-alpha lambda-pir | Y | E. coli host strain DH5-alpha lambda-pir |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
| Author Name | Author Type |
| John Mekalanos | Academic researcher, clone PI |
| Su Chiang | Academic researcher, clone donor |
Publications:
| PMID | Title |
| 7846034 | Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host |
| 9023360 | Bacterial infection as assessed by in vivo gene expression |
