Clone ID:	EvNO00061626
Is Verified:	U
Status:	AVAILABLE
Source:	Mekalanos lab
Description:	Vector for bacterial in vivo expression technology (IVET) transposon insert-based approach to identification of promoters that drive expression of promotorless marker in the vector; amp resistance; restriction enzyme cloning.
Comments:
Type:	No insert
Verification Method:
Distribution:	Academic and non-profit labs
Special MTA:

Map:

Related Identifiers:

SYNONYM	pIVET8 in DH5-alpha lambda-pir
Original Clone ID	pIVET8
SYNONYM	Mekalanos Strain C5

Sequencing Information:

Phred Score:	835
Date:	2018-03-28

Last Passing Sequencing Read:

NNAACTTCTTTAGATAACAAT::GCTGAAAAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCCTTTTTGTCCGGTGTTGGGTTGAAGGTGAAGCCGGTCGGGGCCGCAGCGGGGGCCGGCTTTTCAGCCTTGCCCCCCTGCTTCGGCCGCCGTGGCTCCGGCGTCTTGGGTGCCGGCGCGGGTTCCGCAGCCTTGGCCTGCGGTGCGGGCACATCGGCGGGCTTGGCCTTGATGTGCCGCCTGGCGTGCGAGCGGAACGTCTCGTAGGAGAACTTGACCTTCCCCGTTTCCCGCATGTGCTCCCAAATGGTGACGAGCGCATAGCCGGACGCTAACGCCGCCTCGACATCCGCCCTCACCGCCAGGAACGCAACCGCAGCCTCATCACGCCGGCGCTTCTTGGCCGCGCGGGATTCAACCCACTCGGCCAGCTCGTCGGTGTAGCTCTTTGGCATCGTCTCTCGCCT:GTCCCCCTCGTTCAGTAATTTCCTGCATTTGCCTGTTTCCAATCGGTAGATATTCCACAAAACAGCAGGGAAGCAGCGCTTTTTCGCTGCATACCCCTGCTTCCGGGTCATTAAAACCAATTTTTTCGGTAATACCATTCCTTTTTCGACGAAAATACAGGATTT:GCCAAAGGGGTCCCGGAAAAATTTCCTTGGGGAACCCACAGGGCTCCCCCCGGCAAGAATAAGTAAATAAGGCCCCCCGGGAAAGGGGGGATCCTTCCTCCTGTGCCCCTTATCCCCCCCGGGGGGGCCCCACGAGAAACCGGCTTTTTAGAGCTTGGCCGTTTCTCCCGCGTTAAACATAGAAGCCAAAGAATAGTCTTATAAACATCTCCAACCAGAGAGGGGCCCCCCTCTCTAGAGTTATCCCTTTCAATAAACAAACAAA

Vector Information:

Vector Name:	pIVET8
Type:	bacterial plasmid
Description:	Vector for bacterial in vivo expression technology (IVET) transposon insert-based approach to identification of promoters that drive expression of promotorless marker in the vector; amp resistance; restriction enzyme cloning.
Properties:	bacterial expression, promoter trap, transposon mediated gene disruption
Comments:
Map:
Sequence:
Size (bp):	0
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
DH5-alpha lambda-pir	Y	E. coli host strain DH5-alpha lambda-pir

Antibiotic Selections:

Host Type	Marker
bacterial	ampicillin

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	100 ug/mL ampicillin	Growth with the single antibiotic in LB at 37 degrees is recommended.	Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name	Author Type
John Mekalanos	Academic researcher, clone PI
Su Chiang	Academic researcher, clone donor

Publications:

PMID	Title
7846034	Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host
9023360	Bacterial infection as assessed by in vivo gene expression