Clone: EvNO00061624
| Clone ID: | EvNO00061624 |
| Is Verified: | U |
| Status: | AVAILABLE |
| Source: | Mekalanos lab |
| Description: | Vector that accepts insert for gene targeting approach in bacteria; amp, streptomycin (tetracycline? See map); restriction enzyme cloning. |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
| Map: | pGP704 |
Related Identifiers:
| SYNONYM | GP704 in BW19851 |
| Original Clone ID | pGP704 |
| SYNONYM | Mekalanos Strain C1 |
Sequencing Information:
| Phred Score: | 802 |
| Date: | 2018-04-23 |
Last Passing Sequencing Read:
>29051-32_EvNO00061624-pAmp-5out_H4 802 1519 68 877 NNGCAGCGTGTCAGCGTTCTGGGTGACAAAACAGGAATGCAAAATGCCGC AAAAAGGGAATAATGGCGACACGGAAATGTTGAATACTCATACTCTTCCT TTTTAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATA CATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACAT TTCCCCGAAAAGTGCCACCTGCAGATCTGCAGGTCGACGGATCCCAAGCT TCTTCTAGAGGTACCGCATGCGATATCGAGCTCTCCCGGGAATTCCACAA ATTGTTATCCGCTCACAATTCCACATGTGGAATTCCACATGTGGAATTCC CATGTCAGCCGTTAAGTGTTCCTGTGTCACTCAAAATTGCTTTGAGAGGC TCTAAGGGCTTCTCAGTGCGTTACATCCCTGGCTTGTTGTCCACAACCGT TAAACCTTAAAAGCTTTAAAAGCCTTATATATTCTTTTTTTTCTTATAAA ACTTAAAACCTTAGAGGCTATTTAAGTTGCTGATTTATATTAATTTTATT GTTCAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTTAG CCATGAGAGCTTAGTACGTTAGCCATGAGGGTTTAGTTCGTTAAACATGA GAGCTTAGTACGTTAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTT AGTACGTACTATCAACAGGTTGAACTGCTGATCTTCAGATCCTCTACGCC GGACGCATCGTGGCCGGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGC CGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTG ATTGTCACGCTCAAGCCCGAAGTCCCGTTCGAGCGTCGCGCAAAGGTCAG CGAGGGCGCGGATAGCCCGATACGGCTCATGGATGGTGTTTCGGGTCGGG TGAATCTTGTTGATGGCGATATGGATGTGCAGGTTGTCGGTGTCCTGATG CACGGCACTGACCCGCTGATGCTCGCCGAACCCACCCCAGCGCAGATGCT GTCCTCATCCCGCGCAACGTCTCCGCGTCGGGCTTTCTCCCGGCGGAAGT AACCCACAGGGAATAGTCTTGGCGTTCGAACGGGTGTTCCGTGTTGGTCC CCTACCTCCCCCAGAAACGGAAGGGGTTTCCTCCAATCGGGACCCAAATA CCAGGCCTCGGCTGCTTGCTCCCGGAATACTTCCAGCTCCCAAACCTCTT TTAGAACCAGGGGAAGCTTGGACACCCCCCCCGCGCCTTAAGTAAAAATT GTCTTCCCGGGAAACCCCTAACTTCAATTTCTTTTCTTTTTCGAGGGGAA TGGTCCAAACCCGGGGTCTGAAAATTAAGCCCGGCCTTCTTGGCCCTGGT TTATCCCTTATTCCGAAAGAGAGAGGGCGCTTCTTTCGGAACAAGGCCTG TTCTCCTCTCTCCCCACATACAAAGAAAGTTCTCAAAATAAAATTAGAGG
Vector Information:
| Vector Name: | pGP704 |
| Type: | bacterial plasmid |
| Description: | Vector that accepts insert for gene targeting approach in bacteria; ampicillin, streptomycin (tetracycline? See map); restriction enzyme cloning. |
| Properties: | bacterial expression, gene targeting |
| Comments: | |
| Map: | pGP704.gif |
| Sequence: | pGP704_seq.doc |
| Size (bp): | 3705 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| BW19851 | Y | E. coli host strain BW19851 |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
| bacterial | streptomycin |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
| Author Name | Author Type |
| John Mekalanos | Academic researcher, clone PI |
| Su Chiang | Academic researcher, clone donor |
Publications:
| PMID | Title |
| 2836362 | A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR |
| 728998 | Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K |
