Clone ID:	EvNO00061624
Is Verified:	U
Status:	AVAILABLE
Source:	Mekalanos lab
Description:	Vector that accepts insert for gene targeting approach in bacteria; amp, streptomycin (tetracycline? See map); restriction enzyme cloning.
Comments:
Type:	No insert
Verification Method:
Distribution:	Academic and non-profit labs
Special MTA:

Map:

pGP704

Related Identifiers:

SYNONYM	GP704 in BW19851
Original Clone ID	pGP704
SYNONYM	Mekalanos Strain C1

Sequencing Information:

Phred Score:	802
Date:	2018-04-23

Last Passing Sequencing Read:

>29051-32_EvNO00061624-pAmp-5out_H4 802 1519 68 877 NNGCAGCGTGTCAGCGTTCTGGGTGACAAAACAGGAATGCAAAATGCCGC AAAAAGGGAATAATGGCGACACGGAAATGTTGAATACTCATACTCTTCCT TTTTAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATA CATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACAT TTCCCCGAAAAGTGCCACCTGCAGATCTGCAGGTCGACGGATCCCAAGCT TCTTCTAGAGGTACCGCATGCGATATCGAGCTCTCCCGGGAATTCCACAA ATTGTTATCCGCTCACAATTCCACATGTGGAATTCCACATGTGGAATTCC CATGTCAGCCGTTAAGTGTTCCTGTGTCACTCAAAATTGCTTTGAGAGGC TCTAAGGGCTTCTCAGTGCGTTACATCCCTGGCTTGTTGTCCACAACCGT TAAACCTTAAAAGCTTTAAAAGCCTTATATATTCTTTTTTTTCTTATAAA ACTTAAAACCTTAGAGGCTATTTAAGTTGCTGATTTATATTAATTTTATT GTTCAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTTAG CCATGAGAGCTTAGTACGTTAGCCATGAGGGTTTAGTTCGTTAAACATGA GAGCTTAGTACGTTAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTT AGTACGTACTATCAACAGGTTGAACTGCTGATCTTCAGATCCTCTACGCC GGACGCATCGTGGCCGGATCCAGCCGACCAGGCTTTCCACGCCCGCGTGC CGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTTCGTG ATTGTCACGCTCAAGCCCGAAGTCCCGTTCGAGCGTCGCGCAAAGGTCAG CGAGGGCGCGGATAGCCCGATACGGCTCATGGATGGTGTTTCGGGTCGGG TGAATCTTGTTGATGGCGATATGGATGTGCAGGTTGTCGGTGTCCTGATG CACGGCACTGACCCGCTGATGCTCGCCGAACCCACCCCAGCGCAGATGCT GTCCTCATCCCGCGCAACGTCTCCGCGTCGGGCTTTCTCCCGGCGGAAGT AACCCACAGGGAATAGTCTTGGCGTTCGAACGGGTGTTCCGTGTTGGTCC CCTACCTCCCCCAGAAACGGAAGGGGTTTCCTCCAATCGGGACCCAAATA CCAGGCCTCGGCTGCTTGCTCCCGGAATACTTCCAGCTCCCAAACCTCTT TTAGAACCAGGGGAAGCTTGGACACCCCCCCCGCGCCTTAAGTAAAAATT GTCTTCCCGGGAAACCCCTAACTTCAATTTCTTTTCTTTTTCGAGGGGAA TGGTCCAAACCCGGGGTCTGAAAATTAAGCCCGGCCTTCTTGGCCCTGGT TTATCCCTTATTCCGAAAGAGAGAGGGCGCTTCTTTCGGAACAAGGCCTG TTCTCCTCTCTCCCCACATACAAAGAAAGTTCTCAAAATAAAATTAGAGG

Vector Information:

Vector Name:	pGP704
Type:	bacterial plasmid
Description:	Vector that accepts insert for gene targeting approach in bacteria; ampicillin, streptomycin (tetracycline? See map); restriction enzyme cloning.
Properties:	bacterial expression, gene targeting
Comments:
Map:	pGP704.gif
Sequence:	pGP704_seq.doc
Size (bp):	3705
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
BW19851	Y	E. coli host strain BW19851

Antibiotic Selections:

Host Type	Marker
bacterial	ampicillin
bacterial	streptomycin

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	100 ug/mL ampicillin	Growth with the single antibiotic in LB at 37 degrees is recommended.	Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name	Author Type
John Mekalanos	Academic researcher, clone PI
Su Chiang	Academic researcher, clone donor

Publications:

PMID	Title
2836362	A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
728998	Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K