Clone: EvNO00061622 ← Click Here To Order

Clone ID:EvNO00061622
Is Verified:U
Status:AVAILABLE
Source:Mekalanos lab
Description:Vector for transposon-mediated mutagenesis in bacteria, with Tn-magellan1 element; ampicillin and kanamycin resistance (kanamycin for selection of transposon insertions); restriction enzyme cloning.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:
Map:pEnt3.pdf

Related Identifiers:

SYNONYM pEnt3 in DH5-alpha
Original Clone ID pEnt3
SYNONYM Mekalanos Strain C37

Sequencing Information:

Phred Score: 0
Date: 2012-04-13

Last Passing Sequencing Read:

CANCGCATANATNTTTTTTGTNANAGAGTGACCCGCGTCNNNTGCNACACANTGGAGAGCANCTGTTNTTTTTTNNTGCC ANCGTNTGTTACAAAAAAGNTGNCNTNCATCNTCTGCNCNNCNGNGNGNCGNNCGCGGTNGTNGNNNCANNTNNCATACA NNNGANCCGTGNTGTGCTCACGNTGTCAANCCNTATNTGNANGTCTGNCCGTGGGATNATCACNGGTATCGGTGCGANNG NACCCGCACAGNTTNTCAANGAGCGAAAACTGATNCAACANNCGAGNTNTTGACAANAATACANTTCTTTCTGAAGTCNA NTNCAAACGCGNTGNNCTAGNTAANTACCCAGNTCAGGGNATNTNGGANCATTGTGAATGNCNTNCCCTNCNTTCTTAAT GCTNNCGNCAGTATANNTGTNNGACANCTATGATCNACATTTGATTGANNNNCCACCAAGCTTACNATGCNNANTATGNC TACGANCGNNNGANAGCCNNCTCNAACCCCTNGTANTANNTTATANTCANACCNCCTGTGNNNGATNNTNGNACNACTCN CTNGTCTGNCAAAGGNNGNAACCGGCTTTCNGATTCANATGAGNTTGCNTACANNNTATGAGNNCTACCNNGATCGTNTA TCCNTGATCCACAGNGCTAACACATGTNTGTTTGCATTCTTTGCCCAGCACTTCACGCATCNCTGNTTCAAGACGGATCA TNAGNTNAAGGACATATNTNCTTCCANTGGGNGNCCCNCGTGGGGNGGACTTATNTCTNAGNATGCGGAGACNCTCNNGN NAGANCACCTAAACNGCGGCTTTTCCATNAAGGCCNACTGNAAATATCAGACAATTAGNTNNACCNGAGGTNCCNCCNNC CTTNTAAGGNNCCTNCNNAACAAGAGCATCTANCCTCCNCNNTNCCCNGGAACNATTTACCGNTNTANANGGGGNNCNNN AGGTCTTTTGGNACNGGNCCCTNATTCGGATCAAGTNTGACAAAAACGTANCCGNNGNNNNACANNGAAAAATGCNATGT GCTTAAANNANNACNATCTGGTTGGNNNAATNNNNNAANAANCCCCNTNNNNGGGCCAANNCCNCNTTNNANGTNTTTNC CCTATNGNGNTCGAAAAAAAGGNNNCCNNAGNNGGCNGGGTNACCCTTCCCCCNGAATTTTAGCNNGACCCCATTCCCCC AAAAGTTTNCNNCCCAAAACNGNAGGNANGGNATTNTCCCCCCCNGNAAAANAAAANCCTGGGTTTTTTCTTNNANGAAG GGGGGGAAAAAAAAATAANNNTTTTTTTTNNCCCNAAATTATGGNGAAANANTTNTCNNTNCNCCCCCCCCCNANATATT TGGTGGGNTTNNNNNGAAACTCTTCCCCNCAAAAAAAAATTTTCTNNTT

Vector Information:

Vector Name:pEnt3
Type:bacterial plasmid
Description:Vector for transposon-mediated mutagenesis in bacteria, with Tn-magellan1 element; ampicillin and kanamycin resistance (kanamycin for selection of transposon insertions); restriction enzyme cloning.
Properties:mutagenesis vector, transposon mediated gene disruption
Comments:
Map:pEnt3.pdf
Sequence:pEnt3.txt
Size (bp):0
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha Y E. coli host strain DH5-alpha (Mekalanos lab)

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
bacterial kanamycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
John Mekalanos Academic researcher, clone PI
Su Chiang Academic researcher, clone donor

Publications:

PMID Title
10500193 Hyperactive transposase mutants of the Himar1 mariner transposon
9671781 Systematic identification of essential genes by in vitro mariner mutagenesis