Clone: EvNO00061616
| Clone ID: | EvNO00061616 |
| Is Verified: | U |
| Status: | AVAILABLE |
| Source: | Lee lab (HGTI/HMS) |
| Description: | Helper plasmid for lentiviral vector, rev1b driven by CMV promoter; amp resistance; not a cloning vector. |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
Related Identifiers:
| Original Clone ID | pRC/CMV-rev1b |
| SYNONYM | pRC/CMV-rev1b |
Sequencing Information:
| Phred Score: | 886 |
| Date: | 2017-06-08 |
Last Passing Sequencing Read:
>27367-65_EvNO00061616-CMV-F_A9 886 1394 39 953 NNNNNNNCGTTGGCTTACGAGCTCCTGGCTACTAGAGAACCCACTGCTTA CTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTGGT ACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCA GATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGACTGCCATGG CAGGAAGAAGCGGAGACAGCGACGAAGACCTCCTCAAGGCAGTCAGACTC ATCAAGTTTCTCTATCAAAGCAACCCACCTCCCAACCCCGAGGGGACCCG ACAGGCCCGAAGGAATAGAAGAAGAAGGTGGAGAGAGAGACAGAGACAGA TCCATTCGATTAGTGAACGGATCCTTAGCACTTATCTGGGACGATCTGCG GAGCCTGTGCCTCTTCAGCTACCACCGCTTGAGAGACTTACTCTTGATTG TAACGAGGATTGTGGAACTTCTGGGACGCAGGGGGTGGGAAGCCCTCAAA TATTGGTGGAATCTCCTACAGTATTGGAGTCAGGAACTAAAGAATAGGAT CCGGCTATTCTATAGTGTCACCTAAATGCTAGAGCTCGCTGATCAGCCTC GACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGC CTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAAT GAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGG TGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGC ATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGC TGGGGCTCGAGGGGGGATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGC GGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGNCAGCGCCC TAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCTTTCTCNGCACGTTCGCCG GCTTTCCCCCGTCAGCTCTAAATCGGGGGCTCCCTTAAAGGGTTCGATTT AGTGCTTTACGGCACCTCACCCCNAAAACTTGATTAAGGTGATGTTTCAG TANTGGCCCTCGCCCTGATAGACGGTTTTCGCCCTTTGACGTGGAGTCAC GTTCTTAAAAGTGAACTCTGGTCCAACTGAAACACCTCAACCTATCTCGG CAATCCTTTGATTTAAAGGATTTGCCGATTCGCCCTTGGTTAAAAATGAC TATTTAAAAAATTTCCCCAATTTACAAAATTAACCTCAAATTAAATTTGC TAAACACCTCCGTTTGGGGCTTTCAATACCCGGGGGGGCCAGACAAATTG
Vector Information:
| Vector Name: | pRC/CMV-rev1b |
| Type: | bacterial plasmid |
| Description: | Helper plasmid for lentiviral vector, rev1b driven by CMV promoter; amp resistance; not a cloning vector. |
| Properties: | lentiviral helper, lentiviral infection, mammalian expression, mammalian transduction |
| Comments: | |
| Map: | pRC-CMV-rev1b.pdf |
| Sequence: | pRC-CMV-rev1b.txt |
| Size (bp): | 5900 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| EB5-alpha T1, T5 phage resistant | Y | EB5-alpha host as supplied by Edge BioSystems |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
| mammalian | neomycin |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
| Author Name | Author Type |
| Jeng-Shin Lee | Adademic researcher, clone PI |
