Clone: EvNO00061608 ← Click Here To Order

Clone ID:EvNO00061608
Is Verified:U
Status:AVAILABLE
Source:Lee lab (HGTI/HMS)
Description:Helper plasmid for lentiviral vector, HIV tat driven by CMV promoter; amp resistance; not a cloning vector.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pHDM-tat1b
SYNONYM pHDM-tat1b

Sequencing Information:

Phred Score: 902
Date: 2017-06-09

Last Passing Sequencing Read:

EvNO00061608-2-CMV-F_B3 902 1444 35 969 NNNTTTCATGGGCTTAGAGACTCGTTAGTGACCGTCAGATCGCCTGGAGA CGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAG CCTCCCCTCGAAGCTGATCCTGAGAACTTCAGGGTGAGTCTATGGGACCC TTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAGGA AGGGGAGAAGTAACAGGGTACACATATTGACCAAATCAGGGTAATTTTGC ATTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTA TCTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGAT ACAATGTATCATGCCTCTTTGCACCATTCTAAAGAATAACAGTGATAATT TCTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATAT AAATTGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATC CAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTC TGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTC CTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTT TGGCAAAGAATTCCGCGGGCGGCCGCGAAATGGAGCCAGTAGATCCTAGA CTAGAGCCCTGGAAGCATCCAGGAAGTCAGCCTAAAACTGCTTGTACCAC TTGCTATTGTAAAAAGTGTTGCTTTCATTGCCAAGTTTGTTTCACAACAA AAGCCTTAGGCATCTCCTATGGCAGGAAGAAGCGGAGACAGCGACGAAGA CCTCCTCAAGGCAGTCAGACTCATCAAGTTTCTCTATCAAAGCAACCCAC CTCCCAACCCCGAGGGGACCCGACAGGCCCGAAGAATAGGATCCAAGCTT ATCGATACCGTCGACCTCGAGGGCCCAGATCTAATTCACCCCACAGTGCA GGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGNCCCACA GTATCACTAAGCTCGCTTTCTGCTGTCCATTTNCTATAANGTTCNTTTGN TCCTTAGTCCACTACTAACTGGGGGATATATGAAGGGCTTGAGCATCGGN ATCTGCCTATAAAAAACATTATTTCATTGCATGATGTATTAAATATTTCT GAATTTTTACTAAAAGGAATGGGGNAGGTCATGCTTTTAAACTAAGAAAT GAGAGCTGTTCAACTCTGGAAAAACCTATACTTAACTCTGGAAAAGGGAG GCTCCAACCTAGTGCTTGGGAACCCCTAGCGCTTCCTTTTCTCTTAAAAA ATAAAAAGGTGTTGGAGGAAAATCCCCCCGTATTATATATGTTTGGGCCG

Vector Information:

Vector Name:pHDM-tat1b
Type:bacterial plasmid
Description:Helper plasmid for lentiviral vector, HIV tat driven by CMV promoter; amp resistance; not a cloning vector.
Properties:lentiviral helper, lentiviral infection, mammalian expression, mammalian transduction
Comments:
Map:pHDM-tat1b.pdf
Sequence:pHDM-tat1b.txt
Size (bp):4827
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
EB5-alpha T1, T5 phage resistant Y EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Jeng-Shin Lee Adademic researcher, clone donor and PI