Clone: EvNO00061598 ← Click Here To Order

Clone ID:EvNO00061598
Is Verified:U
Status:AVAILABLE
Source:Lee lab (HGTI/HMS)
Description:Adeno-associated viral (AAV) vector with CMV promoter, SV40 polyA signal, maximum capacity for insertion 2.3 kb, has IRES-ZsGreen (IRES-fluorescent tag) as marker of transduced cells; amp resistance; restriction enzyme cloning.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pAAV-MCS6-IZsGreen
SYNONYM pAAV-MCS6-IZsGreen

Sequencing Information:

Phred Score: 898
Date: 2016-10-27

Last Passing Sequencing Read:

NNNAAACAGTGATATTACAACTCGTTAGTGACCGTCGATCGCCTGGAGAC GCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGC CTCCCCTCGAAGCTGATCCTGAGAACTTCAGGGTGAGTCTATGGGACCCT TGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAGGAA GGGGAGAAGTAACAGGGTACACATATTGACCAAATCAGGGTAATTTTGCA TTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTAT CTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATA CAATGTATCATGCCTCTTTGCACCATTCTAAAGAATAACAGTGATAATTT CTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATATA AATTGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCC AGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTCT GAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTCC TCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTT GGCAAAGAATTCCGCGGGCCCGGGATCCACCGGTCGCCACCATGGTGAGC AAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGA CGGCGACGTANACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCG ATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAG CTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTNGACTACGGCGTGCA GTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGT CCCGCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTTCTCAAGGAC GACNNGCACTACAAGACCCGCGCCGAAGTGAAGTTCGAGGGCGAACACCT GGTGAACCGCATCGAGCTGAAGGGCATCGACTTCNNAGGAGACGGGCACA TCCTGGGGCACCAGCTGGAGTAAACTACNACAGCCACACGTCTATTCATG GCCGACAGCAGAAGACGGCATCANGTGAACTTCAGATCCGCCACACATCG AGAACGGAGCTGAANTTGGCGACACTTACAGAAACACCCCATGGGAAGGG CCCTGGTGTTTCGAAAACCCATACTGAAACCATTCGCCTTAAAAAACCCA CGAAAACGCATCCTGGGCTTCTGGATTTGGAACCCCCGGAATCCCCGAAT

Vector Information:

Vector Name:pAAV-MCS6-IZsGreen
Type:bacterial plasmid
Description:Adeno-associated viral (AAV) vector with CMV promoter, SV40 polyA signal, maximum capacity for insertion 2.3 kb, has IRES-ZsGreen (IRES-fluorescent tag) as marker of transduced cells; amp resistance; restriction enzyme cloning.
Properties:adeno-associated (AAV) expression, fluorescent marker, mammalian expression, mammalian transduction, multiple cloning site, with tag/fusion/marker
Comments:Each time the plasmid is replicated and amplified, should check with a SmaI digest to ensure that the full plasmid is intact. XLBlue cells recommended over DH5-alpha. ZsGreen like eGFP but brighter (statement not confirmed at DNA Resource Core).
Map:pAAV-MCS6-IZsGreen.pdf
Sequence:pAAV-MCS6-IZsGreen.txt
Size (bp):6111
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
XL Blue Y XL Blue cells from Stratagene (XL Blue recommended by donor)

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Jeng-Shin Lee Adademic researcher, clone donor and PI