Clone: EvNO00061596
| Clone ID: | EvNO00061596 |
| Is Verified: | U |
| Status: | AVAILABLE |
| Source: | Lee lab (HGTI/HMS) |
| Description: | Adeno-associated viral (AAV) vector with CMV promoter, beta globin intron and polyA signal, maximum capacity for insertion 2.3 kb; amp resistance; restriction enzyme cloning. |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
| Map: | pAAV-MCS.pdf |
Related Identifiers:
| Original Clone ID | pAAV-MCS |
| SYNONYM | pAAV-MCS |
Sequencing Information:
| Phred Score: | 920 |
| Date: | 2018-02-14 |
Last Passing Sequencing Read:
>28643-25_EvNO00061596-1-CMV-F_A4 920 1381 35 977 NNNGGACATGGACATACAAACTCGTTAGTGACCGTCAGATCGCCTGGAGA CGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAG CCTCCCCTCGAAGCTGATCCTGAGAACTTCAGGGTGAGTCTATGGGACCC TTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAGGA AGGGGAGAAGTAACAGGGTACACATATTGACCAAATCAGGGTAATTTTGC ATTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTA TCTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGAT ACAATGTATCATGCCTCTTTGCACCATTCTAAAGAATAACAGTGATAATT TCTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATAT AAATTGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATC CAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTC TGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTC CTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTT TGGCAAAGAATTCCGCGGGCGGCCGCTCTAGAGGATCCAAGCTTATCGAT ACCGTCGACCTCGAGGGCCCAGATCTAATTCACCCCACCAGTGCAGGCTG CCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTAT CACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTT CCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCAT CTGGATTCTGCCTAATAAAAACATTTATTTTCATTGCAATGATGTATTTA AATTATTTCTGAATATTTTACTAAAAAGGGAATGTGGGAGGTCAGTGCAT TTAAACATAAAGAAATGAAGAGCTAGTTCAAACTTTGGGAAATACACTAT ATCTTAAACTCCATGAAGAAANGTGAGCTGCAAACAGCTAATGCACATTG GCACAGCCCCTGATGCTATGCCTAATTCATCCTCAAAAAAGATTCAGTAA AGCCTGATTGGNAGNTAAAGTTTGCTAGCCGATTTACATACTAATGNTTA CCTGCCTCAGAAGGCTTTCACTACATTGGTTACTGATCCTCAACTGAATC ATAGTCCTGCTAAAAACCACTTTCCTGATGTCTCCAGTTTACGGAATGTT TCCCCCGGCCTCACTTATGCCTGGCTTAAAGCCTTTAAAGAAAAGGCAGG
Vector Information:
| Vector Name: | pAAV-MCS |
| Type: | bacterial plasmid |
| Description: | Adeno-associated viral (AAV) vector with CMV promoter, beta globin intron and polyA signal, maximum capacity for insertion 2.3 kb; amp resistance; restriction enzyme cloning. |
| Properties: | mammalian transduction |
| Comments: | Each time the plasmid is replicated and amplified, should check with a SmaI digest to ensure that the full plasmid is intact. XLBlue cells recommended instead of DH5-alpha. |
| Map: | pAAV-MCS.pdf |
| Sequence: | pAAV-MCS.txt |
| Size (bp): | 6150 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| XL Blue | Y | XL Blue cells from Stratagene (XL Blue recommended by donor) |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
| Author Name | Author Type |
| Jeng-Shin Lee | Adademic researcher, clone donor and PI |
