Clone ID:	EvNO00061596
Is Verified:	U
Status:	AVAILABLE
Source:	Lee lab (HGTI/HMS)
Description:	Adeno-associated viral (AAV) vector with CMV promoter, beta globin intron and polyA signal, maximum capacity for insertion 2.3 kb; amp resistance; restriction enzyme cloning.
Comments:
Type:	No insert
Verification Method:
Distribution:	Academic and non-profit labs
Special MTA:

Map:	pAAV-MCS.pdf

Related Identifiers:

Original Clone ID	pAAV-MCS
SYNONYM	pAAV-MCS

Sequencing Information:

Phred Score:	920
Date:	2018-02-14

Last Passing Sequencing Read:

>28643-25_EvNO00061596-1-CMV-F_A4 920 1381 35 977 NNNGGACATGGACATACAAACTCGTTAGTGACCGTCAGATCGCCTGGAGA CGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAG CCTCCCCTCGAAGCTGATCCTGAGAACTTCAGGGTGAGTCTATGGGACCC TTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAGGA AGGGGAGAAGTAACAGGGTACACATATTGACCAAATCAGGGTAATTTTGC ATTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTA TCTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGAT ACAATGTATCATGCCTCTTTGCACCATTCTAAAGAATAACAGTGATAATT TCTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATAT AAATTGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATC CAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTC TGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTC CTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTT TGGCAAAGAATTCCGCGGGCGGCCGCTCTAGAGGATCCAAGCTTATCGAT ACCGTCGACCTCGAGGGCCCAGATCTAATTCACCCCACCAGTGCAGGCTG CCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTAT CACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTT CCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCAT CTGGATTCTGCCTAATAAAAACATTTATTTTCATTGCAATGATGTATTTA AATTATTTCTGAATATTTTACTAAAAAGGGAATGTGGGAGGTCAGTGCAT TTAAACATAAAGAAATGAAGAGCTAGTTCAAACTTTGGGAAATACACTAT ATCTTAAACTCCATGAAGAAANGTGAGCTGCAAACAGCTAATGCACATTG GCACAGCCCCTGATGCTATGCCTAATTCATCCTCAAAAAAGATTCAGTAA AGCCTGATTGGNAGNTAAAGTTTGCTAGCCGATTTACATACTAATGNTTA CCTGCCTCAGAAGGCTTTCACTACATTGGTTACTGATCCTCAACTGAATC ATAGTCCTGCTAAAAACCACTTTCCTGATGTCTCCAGTTTACGGAATGTT TCCCCCGGCCTCACTTATGCCTGGCTTAAAGCCTTTAAAGAAAAGGCAGG

Vector Information:

Vector Name:	pAAV-MCS
Type:	bacterial plasmid
Description:	Adeno-associated viral (AAV) vector with CMV promoter, beta globin intron and polyA signal, maximum capacity for insertion 2.3 kb; amp resistance; restriction enzyme cloning.
Properties:	mammalian transduction
Comments:	Each time the plasmid is replicated and amplified, should check with a SmaI digest to ensure that the full plasmid is intact. XLBlue cells recommended instead of DH5-alpha.
Map:	pAAV-MCS.pdf
Sequence:	pAAV-MCS.txt
Size (bp):	6150
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
XL Blue	Y	XL Blue cells from Stratagene (XL Blue recommended by donor)

Antibiotic Selections:

Host Type	Marker
bacterial	ampicillin

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	100 ug/mL ampicillin	Growth with the single antibiotic in LB at 37 degrees is recommended.	Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name	Author Type
Jeng-Shin Lee	Adademic researcher, clone donor and PI