Clone: EvNO00061595 ← Click Here To Order

Clone ID:EvNO00061595
Is Verified:U
Status:AVAILABLE
Source:Lee lab (HGTI/HMS)
Description:Adeno-associated viral (AAV) vector, CMV promoter drives GFP fluorescent marker; amp resistance; restriction enzyme cloning.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pAAV-GFP
SYNONYM pAAV-GFP

Vector Information:

Vector Name:pAAV-GFP
Type:bacterial plasmid
Description:Adeno-associated viral (AAV) vector, CMV promoter drives GFP fluorescent marker; amp resistance; restriction enzyme cloning.
Properties:mammalian transduction
Comments:Each time the plasmid is replicated and amplified, should check with a SmaI digest to ensure that the full plasmid is intact. XLBlue cells recommended instead of DH5-alpha.
Map:pAAV-GFP.pdf
Sequence:pAAV-GFP.txt
Size (bp):6940
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
XL Blue Y XL Blue cells from Stratagene (XL Blue recommended by donor)

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Jeng-Shin Lee Adademic researcher, clone donor and PI

Publications:

PMID Title
25798616 NRF2 promotes neuronal survival in neurodegeneration and acute nerve damage