Clone ID:	EvNO00061595
Is Verified:	U
Status:	AVAILABLE
Source:	Lee lab (HGTI/HMS)
Description:	Adeno-associated viral (AAV) vector, CMV promoter drives GFP fluorescent marker; amp resistance; restriction enzyme cloning.
Comments:
Type:	No insert
Verification Method:
Distribution:	Academic and non-profit labs
Special MTA:

Map:	pAAV-GFP.pdf

Related Identifiers:

Original Clone ID	pAAV-GFP
SYNONYM	pAAV-GFP

Sequencing Information:

Phred Score:	915
Date:	2018-06-18

Last Passing Sequencing Read:

NNNNACCGGTGGCATAGCAAACTCGTTAGTGACCGTCAGATCGCCTGGAG ACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCA GCCTCCCCTCGAAGCTGATCCTGAGAACTTCAGGGTGAGTCTATGGGACC CTTGATGTTTTCTTTCCCCTTCTTTTCTATGGTTAAGTTCATGTCATAGG AAGGGGAGAAGTAACAGGGTACACATATTGACCAAATCAGGGTAATTTTG CATTTGTAATTTTAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTT ATCTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGA TACAATGTATCATGCCTCTTTGCACCATTCTAAAGAATAACAGTGATAAT TTCTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATA TAAATTGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAAT CCAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATT CTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTT CCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACT TTGGCAAAGAATTCCGCGGGCGGCCGCTCTAGAGGATCCAAGCTTATCGA TACCGTCGACCTCGAGGGCCCAGATCTAATTCACCCCACCAGTGCAGGCT GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA TCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGT TCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCA TCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGCAATGATGTATT TAAATTATTTCTGAATATTTTACTAANAAGGNAATGTGNNGAGTCAGTGC ATTTAAAACATAAGAATGAAAACTAGTTCAACCTTGGGAAAATAACTATA TCTTAAACTCCTGAAAGAAGGTGAGCTGCAACAGCTAATGCACATGGCAC AGCCCTGATGCTATGCCTATTCTCCCTCGAAAAGATTCAGTAAAGCTTGA TTTGAGTTAAAGTTTGCTAGCTGATTTTACTTACTATTGTTAACGCCCTC AGAAGTCTTCCCNACCATTGCTACCGCATCCCACCTGATCCACCGTTCTG TTAAAACCCTTCCCTGGGTTCTTCCGTTACGAAAAGTTTCCCCCGCCCTC CTATGTCTTTTTAAAGTCTTAAAAAAAGGGGGGTTGTGCTGACCTTCCTT CCCCGGCGAACCCTTAAGGGAAAAAGGGGTATTAAACCAGGGGGCCCCTC CCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNA NNNGNNNNNANNANANAAAAGACTTTCCTTCTTTATAACTTCCCACTCNN

Vector Information:

Vector Name:	pAAV-GFP
Type:	bacterial plasmid
Description:	Adeno-associated viral (AAV) vector, CMV promoter drives GFP fluorescent marker; amp resistance; restriction enzyme cloning.
Properties:	mammalian transduction
Comments:	Each time the plasmid is replicated and amplified, should check with a SmaI digest to ensure that the full plasmid is intact. XLBlue cells recommended instead of DH5-alpha.
Map:	pAAV-GFP.pdf
Sequence:	pAAV-GFP.txt
Size (bp):	6940
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
XL Blue	Y	XL Blue cells from Stratagene (XL Blue recommended by donor)

Antibiotic Selections:

Host Type	Marker
bacterial	ampicillin

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	100 ug/mL ampicillin	Growth with the single antibiotic in LB at 37 degrees is recommended.	Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name	Author Type
Jeng-Shin Lee	Adademic researcher, clone donor and PI

Publications:

PMID	Title
25798616	NRF2 promotes neuronal survival in neurodegeneration and acute nerve damage