Clone: EvNO00483428 ← Click Here To Order

Clone ID:EvNO00483428
Is Verified:Y
Status:AVAILABLE
Source:DRSC
Description:Empty Drosophila expression vector
Comments: http://flyrnai.org/TRiP-HOME.html
Type:No insert
Verification Method:End read identity check
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Vector Information:

Vector Name:pQUAS-WALIUM20
Type:bacterial plasmid
Description:Drosophila expression vector. loxP, and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, 94-bp QUAS, Hsp70 promoter with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA.
Properties:drosophila in vitro and in vivo expression
Comments:Two copies of loxp-5xUAS sequences of "pWALIUM20" are deleted and replaced with the 94-bp QUAS sequence (five copies of the QF binding site)
Map:pQUAS-WALIUM20 Map-corrected.pdf
Sequence:pQUAS-WALIUM20.txt
Size (bp):10372
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha N

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
drosophila white

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.