Clone: EvNO00483428
| Clone ID: | EvNO00483428 |
| Is Verified: | Y |
| Status: | AVAILABLE |
| Source: | DRSC |
| Description: | Empty Drosophila expression vector |
| Comments: | http://flyrnai.org/TRiP-HOME.html |
| Type: | No insert |
| Verification Method: | End read identity check |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
Related Identifiers:
Sequencing Information:
| Phred Score: | 861 |
| Date: | 2018-02-21 |
Last Passing Sequencing Read:
>28688-45_EvNO00483428-5-T7_E6 861 1498 46 902 NNNNAAAGAGCACTCGACTAGCGCGCCCTANTTCAGTGAAATCCAGCATT TTCTAAATTAAATGTATTCTTATTATTATAGTTGTTATTTTTGATATATA TAAACAACACTATTATGCCCACCATTTTTTTGAGATGCATCTACACAAGG AACAAACACTGGATGTCACTTTCAGTTCAAATTGTAACGCTAATCACTCC GAACAGGTCACAAAAAATTACCTTAAAAAGTCATAATATTAAATTAGAAT AAATATAGCTGTGAGGGAAATATATACAAATATATTGGAGCAAATAAATT GTACATACAAATATTTATTACTAATTTCTATTGAGACGAAATGAACCACT CGGAACCATTTGAGCGAACCGAATCGCGCGGAACTAACGACAGTCGCTCC AAGGTCGTCGAACAAAAGGTGAATGTGTTGCGGAGAGCGGGTGGGAGACA GCGAAAGAGCAACTACGAAACGTGGTGTGGTGGAGGTGAATTATGAAGAG GGCGCGCGATTTGAAAAGTATGTATATAAAAAATATATCCCGGTGTTTTA TGTAGCGATAAACGAGTTTTTGATGTAAGGTATGCAGGTGTGTAAGTCTT TTGGTTAGAAGACAAATCCAAAGTCTACTTGTGGGGATGTTCGAAGGGGA AATACTTGTATTCTATAGGTCATATCTTGTTTTTATTGGCACAAATATAA TTACATTAGCTTTTTGAGGGGGCAATAAACAGTAAACACGATGGTAATAA TGGTAAAAAAAAAAAACAAGCAGTTATTTCGGATATATGTCGGCTACTCC TTGCGTCGGGCCCGAAGTCTTAGAGCCAGATATGCGAGCACCCGGAAGCT CACGATGAGAATGGCCAGACCCACGTAGTCCAGCGGCAGATCGGCGGCGG AGAAAGTAAGCGTCTCCAGGATGACCTTGCCCGAAACTGGGCACGTGGTG TTCGACGATGTGCAGCTAATTTTCGCCCGCTCCACGTCCGCCCATTTGGT TATCAGCAGACCCNTCGTGGGCGTACGAACCCATGAGAGTACGACAACCA TTTGAGTTTACTGGCACCGAGCCCGAGTCAGAAAGAGCCGCCAAAGAGCA GAATGTATGAATACCGGCGACCCACAAAAGCCCTTCGAGTCAAGAACTGG CCAGTATTAAAAATCGAAGACGTGAACCTGNCCACAAATGACCCCGCAGC AATAAAAAGCACATCCGCCCAATCGATCCGAAAGCATCCCGGAAACATGC CTGTAAAAACGGATTCGCACCTTGCCAAAGTTGCAACGAAGTCATCGGCC CTATAAAGCGCCTAGGACACCACACTTTTGAGGGAATGAAGAGGTTCAGG TCTTCGAACATAAGCGGGGAAAAAACCTATTCACCGGGTGCAAAAACAAG GCCCAATCCCTTTCTAATTTCCTATAAAAAGGGNAANNNNNNNNNNTTTT
Vector Information:
| Vector Name: | pQUAS-WALIUM20 |
| Type: | bacterial plasmid |
| Description: | Drosophila expression vector. loxP, and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, 94-bp QUAS, Hsp70 promoter with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. |
| Properties: | drosophila in vitro and in vivo expression |
| Comments: | Two copies of loxp-5xUAS sequences of "pWALIUM20" are deleted and replaced with the 94-bp QUAS sequence (five copies of the QF binding site) |
| Map: | pQUAS-WALIUM20 Map-corrected.pdf |
| Sequence: | pQUAS-WALIUM20.txt |
| Size (bp): | 10372 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| DH5-alpha | N |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
| drosophila | white |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
