Clone: EvNO00438083 ← Click Here To Order

Clone ID:EvNO00438083
Is Verified:Y
Status:AVAILABLE
Source:
Description:Packaging plasmid used for viral production. Mammalian expression of Gag-Pol driven by CMV promoter. Ampicillin resistance in bacteria. Map inferred from end read sequence and anecdotal information. Reference sequence is END READ ONLY.
Comments:
Type:No insert
Verification Method:End Read Sequencing with CMV-F
Distribution:Academic and non-profit labs
Special MTA:
Map:pMDL.pdf

Related Identifiers:

Sequencing Information:

Phred Score: 884
Date: 2017-03-27

Last Passing Sequencing Read:

NNNNCCGTGAGCTTAGCGACTCGTTAGTGACCGTCGATCGCCTGGAGACG CCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCC TCCCCTCGAAGCTTACATGTGGTACCGAGCTCGGATCCTGAGAACTTCAG GGTGAGTCTATGGGACCCTTGATGTTTTCTTTCCCCTTCTTTTCTATGGT TAAGTTCATGTCATAGGAAGGGGAGAAGTAACAGGGTACACATATTGACC AAATCAGGGTAATTTTGCATTTGTAATTTTAAAAAATGCTTTCTTCTTTT AATATACTTTTTTGTTTATCTTATTTCTAATACTTTCCCTAATCTCTTTC TTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCTAA AGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATTTCTGCAT ATAAATATTTCTGCATATAAATTGTAACTGATGTAAGAGGTTTCATATTG CTAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTTATGGTTGG GATAAGGCTGGATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATG TTCATACCTCTTATCTTCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTG TGTGCTGGCCCATCACTTTGGCAAAGAATTCATGGGCCAGACTGTTACCA CTCCCTTAAGTTTGACCTTAGGTCACTGGAAAGATGTCGAGCGGATCGCT CACAACCAGTCGGTAGATGTCAAGAAGAGACGTTGGGTTACCTTCTGCTC TGCAGAATGGCCAACCTTTAACGTCGGATGGCCGCGAGACGGCACCTTTA ACCGAGACCTCATCACCCAGGTTAAGATCAAGGTCTTTTCACCTGGCCCG CATGGACACCCAGACCAGGTCCCCTACATCGTGACCTGGGAAGCCTTGGC TTTTGACCCCCCTCCCTGGGTCAAGCCCTTTGTACACCCTAAGCCTCCCG CTCCTCTTTCTCATCCGCCCCGTCTCTCCCCTTGAACCCTCTCGTTCGAC CCCGCTCGATCCTCCCTTATCAGCCNTCACTCTTCTCTAGGGGCAAAACT AACTTCAAGTCCTTCTGAAGTGGGGGGCGCTCATCGACTACTTACGAGAA CCCCCGCTTAAGGGACCCAAACCCCCCTTCCAAAGGACGGAATGGTGGAA ACCACCCTGGGGGAAGGACCGACCCTCCCCAGGGATTTCCCAAAGGGAAA GGGACCCCCGGGGCCACCCAACCTCCAGGTTCTCCCCCGCAGAGGAAGGA

Vector Information:

Vector Name:pMDL
Type:bacterial plasmid
Description:Packaging plasmid used for viral production. Mammalian expression of Gag-Pol driven by CMV promoter. Ampicillin resistance in bacteria. Map inferred from end read sequence and anecdotal information. Reference sequence is END READ ONLY.
Properties:viral production
Comments:
Map:pMDL.pdf
Sequence:pMDL.txt
Size (bp):8895
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha N

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.