Clone: HsCD00043795

Clone ID:HsCD00043795
Is Verified:Y
Status:AVAILABLE
Source:HIP
Description:
Comments:
Type:cDNA
Verification Method:Sequence Verification
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

HIP Clone ID 179332
Original Clone ID FLH179332.01X
HIP Master Clone ID 154433

Property:

Collection: HIP human pDONR221 set

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 999 Homo sapiens CLOSED MGC template 89822 KCNK17 potassium channel, subfamily K, member 17
Insert1
Size (bp)999
SpeciesHomo sapiens
FormatCLOSED
Tissue SourceMGC template
Species Specific ID 89822
Gene SymbolKCNK17
Gene Namepotassium channel, subfamily K, member 17
Keyword

Insert Sequence:

ATGTACCGACCGCGAGCCCGGGCGGCTCCCGAGGGCAGGGTCCGGGGCTGCGCGGTGCCCGGCACCGTGCTCCTGCTGCTCGCCTACCTGGCTTACCTGGCGCTGGGCACCGGCGTGTTCTGGACGCTGGAGGGCCGCGCGGCGCAGGACTCCAGCCGCAGCTTCCAGCGCGACAAGTGGGAGCTGTTGCAGAACTTCACGTGTCTGGACCGCCCGGCGCTGGACTCGCTGATCCGGGATGTCGTCCAAGCATACAAAAACGGAGCCAGCCTCCTCAGCAACACCACCAGCATGGGGCGCTGGGAGCTCGTGGGCTCCTTCTTCTTTTCTGTGTCCACCATCACCACCATTGGCTATGGCAACCTGAGCCCCAACACGATGGCTGCCCGCCTCTTCTGCATCTTCTTTGCCCTTGTGGGGATCCCACTCAACCTCGTGGTGCTCAACCGACTGGGGCATCTCATGCAGCAGGGAGTAAACCACTGGGCCAGCAGGCTGGGGGGCACCTGGCAGGATCCTGACAAGGCGCGGTGGCTGGCGGGCTCTGGCGCCCTCCTCTCGGGCCTCCTGCTCTTCCTGCTGCTGCCACCGCTGCTCTTCTCCCACATGGAGGGCTGGAGCTACACAGAGGGCTTCTACTTCGCCTTCATCACCCTCAGCACCGTGGGCTTCGGCGACTACGTGATTGGAATGAACCCCTCCCAGAGGTACCCACTGTGGTACAAGAACATGGTGTCCCTGTGGATCCTCTTTGGGATGGCATGGCTGGCCTTGATCATCAAACTCATCCTCTCCCAGCTGGAGACGCCAGGGAGGGTATGTTCCTGCTGCCACCACAGCTCTAAGGAAGACTTCAAGTCCCAAAGCTGGAGACAGGGACCTGACCGGGAGCCAGAGTCCCACTCCCCACAGCAAGGATGCTATCCAGAGGGACCCATGGGAATCATACAGCATCTGGAACCTTCTGCTCACGCTGCAGGCTGTGGCAAGGACAGCTAG

Insert Property: Insert 1

Type Value Extra Information
End on reference sequence 1086
Start on reference sequence 88

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCACCATGTACCGACCGCGAGCCCGGGCGGCTCCCGAGGGCAGGGTCCGGGGCTGCGCGGTGCCCGGCACCGTGCTCCTGCTGCTCGCCTACCTGGCTTACCTGGCGCTGGGCACCGGCGTGTTCTGGACGCTGGAGGGCCGCGCGGCGCAGGACTCCAGCCGCAGCTTCCAGCGCGACAAGTGGGAGCTGTTGCAGAACTTCACGTGTCTGGACCGCCCGGCGCTGGACTCGCTGATCCGGGATGTCGTCCAAGCATACAAAAACGGAGCCAGCCTCCTCAGCAACACCACCAGCATGGGGCGCTGGGAGCTCGTGGGCTCCTTCTTCTTTTCTGTGTCCACCATCACCACCATTGGCTATGGCAACCTGAGCCCCAACACGATGGCTGCCCGCCTCTTCTGCATCTTCTTTGCCCTTGTGGGGATCCCACTCAACCTCGTGGTGCTCAACCGACTGGGGCATCTCATGCAGCAGGGAGTAAACCACTGGGCCAGCAGGCTGGGGGGCACCTGGCAGGATCCTGACAAGGCGCGGTGGCTGGCGGGCTCTGGCGCCCTCCTCTCGGGCCTCCTGCTCTTCCTGCTGCTGCCACCGCTGCTCTTCTCCCACATGGAGGGCTGGAGCTACACAGAGGGCTTCTACTTCGCCTTCATCACCCTCAGCACCGTGGGCTTCGGCGACTACGTGATTGGAATGAACCCCTCCCAGAGGTACCCACTGTGGTACAAGAACATGGTGTCCCTGTGGATCCTCTTTGGGATGGCATGGCTGGCCTTGATCATCAAACTCATCCTCTCCCAGCTGGAGACGCCAGGGAGGGTATGTTCCTGCTGCCACCACAGCTCTAAGGAAGACTTCAAGTCCCAAAGCTGGAGACAGGGACCTGACCGGGAGCCAGAGTCCCACTCCCCACAGCAAGGATGCTATCCAGAGGGACCCATGGGAATCATACAGCATCTGGAACCTTCTGCTCACGCTGCAGGCTGTGGCAAGGACAGCTAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Sequencing Information:

Phred Score: 753
Date: 2014-11-17

Last Passing Sequencing Read:

NNNGGGCGCATAGATTTATTTTGATGATGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCACCATGTACCGACCGCGAGCCCGGGCGGCTCCCGAGGGCAGGGTCCGGGGCTGCGCGGTGCCCGGCACCGTGCTCCTGCTGCTCGCCTACCTGGCTTACCTGGCGCTGGGCACCGGCGTGTTCTGGACGCTGGAGGGCCGCGCGGCGCAGGACTCCAGCCGCAGCTTCCAGCGCGACAAGTGGGAGCTGTTGCAGAACTTCACGTGTCTGGACCGCCCGGCGCTGGACTCGCTGATCCGGGATGTCGTCCAAGCATACAAAAACGGAGCCAGCCTCCTCAGCAACACCACCAGCATGGGGCGCTGGGAGCTCGTGGGCTCCTTCTTCTTTTCTGTGTCCACCATCACCACCATTGGCTATGGCAACCTGAGCCCCAACACGATGGCTGCCCGCCTCTTCTGCATCTTCTTTGCCCTTGTGGGGATCCCACTCAACCTCGTGGTGCTCAACCGACTGGGGCATCTCATGCAGCAGGGAGTAAACCACTGGGCCAGCAGGCTGGGGGGCACCTGGCAGGATCCTGACAAGGCGCGGTGGCTGGCGGGCTCTGGCGCCCTCCTCTCGGGCCTCCTGCTCTTCCTGCTGCTGCCACCGCTGCTCTTCTCCCACATGGAGGGCTGGAGCTACACAGAGGGCTTCTACTTCGCCTTCATCACCCTCAGCACCGTGGGCTTCGGCGACTACGTGATTGGAATGAACCCCTCCCAAAGGTACCCACTGTGGTACAAAACATGGTGTCCCTGTGGATCCTCTTTGGGATGGCATGGCTGGGCTTGATCATCAAACTCATCCTCTCCCAGCTGGAAACCCAAGGGAAGGTATGTTCCTGCTGCCCCCACAGCTCTTAAGAAAAACTTCCAGTCCCAAAGCTGGAAGACGGGAACCTGAACCGGAACCAGAATCCCCCCTCCCCCACACAAGGATGCTTTCCCAAAGGACACCCTGGGAAATCTAAAGGCATCTGGAAACCTTTTGCTTCACCCTCCAAGCTTGTGGCAAGGAACACATAAGACCCACTCTTTTTTTTACAAAGTTGGGATTTAAAAAAAGACTTGCCTTACCAATTGTTTTGACACCAAAAAGTCCCTTTTCGGCCCAAATAAAACCCTTTATTTGCCACACCCTGAAATTCCCCTTAATGAGACTCAATAAAGGGGGCAATAAGTTTTCTCGTGGACCCTTGGGCCCGGTTTCTCAAAATTCTTTAGTTTCTTTCGTCCAAGATAAAAAATTTTTCTTTNGAGACAAAAACATGCTCTCCTTTCTAANCAGATAACAAGGGGTGTTGTTTANCTACTCTTCTGGGAAAGCGCAGGCCCCGATATATATCACGCGTGGTGTCCTATG

Vector Information:

Vector Name:pDONR221
Type:bacterial plasmid
Description:Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Properties:Gateway, donor (entry), recombinational cloning
Comments:The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Map:pdonr221_pdonrzeo_map.pdf
Sequence:pDONR221_FASTA.txt
Size (bp):4762
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial kanamycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 50ug/mL kanamycin Growth with the single antibiotic in LB at 37 degrees is recommended. Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Authors:

Author Name Author Type
HIP Academic Institute