Clone: EvNO00432212 ← Click Here To Order

Clone ID:EvNO00432212
Is Verified:N
Status:AVAILABLE
Source:Wilson Endege
Description:Mammalian expression vector for lentiviral work. Fusion format inserts are expressed with self excising fluorescent marker mRFP. LoxP cloning system. Viral components on backbone help expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Vector Information:

Vector Name:pWE-p2A-mRFP
Type:bacterial plasmid
Description:Mammalian expression vector for lentiviral work. Fusion format inserts are expressed with self excising fluorescent marker mRFP. LoxP cloning system. Viral components on backbone help expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines.
Properties:mammalian expression
Comments:
Map:pCW45-2A-mRFP.pdf
Sequence:pCW45-2A-mRFP.txt
Size (bp):8144
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant N

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
mammalian blasticidin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.