Clone: EvNO00422228
| Clone ID: | EvNO00422228 |
| Is Verified: | N |
| Status: | AVAILABLE |
| Source: | DRSC |
| Description: | Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP, and MCS. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, ftz intron, and SV40 polyA. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
Related Identifiers:
Sequencing Information:
| Phred Score: | 833 |
| Date: | 2017-04-21 |
Last Passing Sequencing Read:
NNNNAGGAGCTCGACTAGCGCGCCCAGTTCCAGTGAATCCAGCATTTTCT AAATTAAATGTATTCTTATTATTATAGTTGTTATTTTTGATATATATAAA CAACACTATTATGCCCACCATTTTTTTGAGATGCATCTACACAAGGAACA AACACTGGATGTCACTTTCAGTTCAAATTGTAACGCTAATCACTCCGAAC AGGTCACAAAAAATTACCTTAAAAAGTCATAATATTAAATTAGAATAAAT ATAGCTGTGAGGGAAATATATACAAATATATTGGAGCAAATAAATTGTAC ATACAAATATTTATTACTAATTTCTATTGAGACGAAATGAACCACTCGGA ACCATTTGAGCGAACCGAATCGCGCGGAACTAACGACAGTCGCTCCAAGG TCGTCGAACAAAAGGTGAATGTGTTGCGGAGAGCGGGTGGGAGACAGCGA AAGAGCAACTACGAAACGTGGTGTGGTGGAGGTGAATTATGAAGAGGGCG CGCGATTTGAAAAGTATGTATATAAAAAATATATCCCGGTGTTTTATGTA GCGATAAACGAGTTTTTGATGTAAGGTATGCAGGTGTGTAAGTCTTTTGG TTAGAAGACAAATCCAAAGTCTACTTGTGGGGATGTTCGAAGGGGAAATA CTTGTATTCTATAGGTCATATCTTGTTTTTATTGGCACAAATATAATTAC ATTAGCTTTTTGAGGGGGCAATAAACAGTAAACACGATGGTAATAATGGT AAAAAAAAAAAACAAGCAGTTATTTCGGATATATGTCGGCTACTCCTTGC GTCGGGCCCGAAGTCTTAGAGCCAGATATGCGAGCACCCGGAAGCTCACG ATGAGAATGGCCAGACCCACGTAGTCCAGCGGCAGATCGGCGGCGGAGAA GTTAAGCGTCTCCAGAATGACCTTGCCNNGAACTGGGCACGTGGTGTTCG ACGATGTGCAGCTAATTTCGCCGGGGCTCACGTCGCCCATTGGTAATCAG CAGACCCTCGTTGGGCGTACGAACCCATGAGAGTACGACACCCATTGAGT ATACTGGCACCGACCCGAGTCAAGAGAACCGCAAAGAGCAGATGTATGAT ACCCGCGACCACAAACACGCATCNAGTCAAGAACTGGCCAGATATAAATT CGAAGACGTGACCATGGCACCAATGAACACGCAGCAATGAAAATGCACCT CGNGCCCATCCGAATCGAAAGCAATCCGGAAACAATGCCTTGGAAAAACG CAATCGCACCTTTCCCAAAGAGGGCACCAAAAACACTCGGCTCCCAAACT GGGCTGGGGAACCAACAACGTTTTAAAGGAAATTGAGGGGGTCAACCTTC TTTTGCAAAATATAGCGGTGGGAAAAAACCCTATTCCCCCCGGGTTGTCA
Vector Information:
| Vector Name: | pWALIUM10-moe |
| Type: | bacterial plasmid |
| Description: | Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP, and MCS. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, ftz intron, and SV40 polyA. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA |
| Properties: | drosophila in vitro and in vivo expression |
| Comments: | Revised 4/25/2012 |
| Map: | WALIUM10moe_map_seq.pdf |
| Sequence: | pWALIUM10-moe.txt |
| Size (bp): | 9842 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| DH5-alpha | N |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
| drosophila | white |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
