Clone ID:	EvNO00422228
Is Verified:	N
Status:	AVAILABLE
Source:	DRSC
Description:	Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP, and MCS. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, ftz intron, and SV40 polyA. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA
Comments:
Type:	No insert
Verification Method:
Distribution:	Academic and non-profit labs
Special MTA:

Map:	VALIUM10moe_map_seq.pdf

Related Identifiers:

Sequencing Information:

Phred Score:	833
Date:	2017-04-21

Last Passing Sequencing Read:

NNNNAGGAGCTCGACTAGCGCGCCCAGTTCCAGTGAATCCAGCATTTTCT AAATTAAATGTATTCTTATTATTATAGTTGTTATTTTTGATATATATAAA CAACACTATTATGCCCACCATTTTTTTGAGATGCATCTACACAAGGAACA AACACTGGATGTCACTTTCAGTTCAAATTGTAACGCTAATCACTCCGAAC AGGTCACAAAAAATTACCTTAAAAAGTCATAATATTAAATTAGAATAAAT ATAGCTGTGAGGGAAATATATACAAATATATTGGAGCAAATAAATTGTAC ATACAAATATTTATTACTAATTTCTATTGAGACGAAATGAACCACTCGGA ACCATTTGAGCGAACCGAATCGCGCGGAACTAACGACAGTCGCTCCAAGG TCGTCGAACAAAAGGTGAATGTGTTGCGGAGAGCGGGTGGGAGACAGCGA AAGAGCAACTACGAAACGTGGTGTGGTGGAGGTGAATTATGAAGAGGGCG CGCGATTTGAAAAGTATGTATATAAAAAATATATCCCGGTGTTTTATGTA GCGATAAACGAGTTTTTGATGTAAGGTATGCAGGTGTGTAAGTCTTTTGG TTAGAAGACAAATCCAAAGTCTACTTGTGGGGATGTTCGAAGGGGAAATA CTTGTATTCTATAGGTCATATCTTGTTTTTATTGGCACAAATATAATTAC ATTAGCTTTTTGAGGGGGCAATAAACAGTAAACACGATGGTAATAATGGT AAAAAAAAAAAACAAGCAGTTATTTCGGATATATGTCGGCTACTCCTTGC GTCGGGCCCGAAGTCTTAGAGCCAGATATGCGAGCACCCGGAAGCTCACG ATGAGAATGGCCAGACCCACGTAGTCCAGCGGCAGATCGGCGGCGGAGAA GTTAAGCGTCTCCAGAATGACCTTGCCNNGAACTGGGCACGTGGTGTTCG ACGATGTGCAGCTAATTTCGCCGGGGCTCACGTCGCCCATTGGTAATCAG CAGACCCTCGTTGGGCGTACGAACCCATGAGAGTACGACACCCATTGAGT ATACTGGCACCGACCCGAGTCAAGAGAACCGCAAAGAGCAGATGTATGAT ACCCGCGACCACAAACACGCATCNAGTCAAGAACTGGCCAGATATAAATT CGAAGACGTGACCATGGCACCAATGAACACGCAGCAATGAAAATGCACCT CGNGCCCATCCGAATCGAAAGCAATCCGGAAACAATGCCTTGGAAAAACG CAATCGCACCTTTCCCAAAGAGGGCACCAAAAACACTCGGCTCCCAAACT GGGCTGGGGAACCAACAACGTTTTAAAGGAAATTGAGGGGGTCAACCTTC TTTTGCAAAATATAGCGGTGGGAAAAAACCCTATTCCCCCCGGGTTGTCA

Vector Information:

Vector Name:	pWALIUM10-moe
Type:	bacterial plasmid
Description:	Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP, and MCS. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, ftz intron, and SV40 polyA. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA
Properties:	drosophila in vitro and in vivo expression
Comments:	Revised 4/25/2012
Map:	WALIUM10moe_map_seq.pdf
Sequence:	pWALIUM10-moe.txt
Size (bp):	9842
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
DH5-alpha	N

Antibiotic Selections:

Host Type	Marker
bacterial	ampicillin
drosophila	white

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	100 ug/mL ampicillin	Growth with the single antibiotic in LB at 37 degrees is recommended.	Commonly used conditions for ampicillin resistant plasmid clones.