Clone: EvNO00422227
| Clone ID: | EvNO00422227 |
| Is Verified: | N |
| Status: | AVAILABLE |
| Source: | DRSC |
| Description: | Drosophila expression vector for Recombination cloning, Over-Expression (ROE). loxP, and GATEWAY recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Two Gypsy insulators, two 5xUAS, Hsp70 promoter, with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
Related Identifiers:
Sequencing Information:
| Phred Score: | 846 |
| Date: | 2016-12-02 |
Last Passing Sequencing Read:
>26372-44_EvNO00422227-M13F_D6 846 1448 31 853 NNNNAAAGATACTATAGGCGATTGGGTACAGCTTCACGACCTAGGCGCGC CCTAGTTCCAGTGAAATCCAAGCATTTTCTAAATTAAATGTATTCTTATT ATTATAGTTGTTATTTTTGATATATATAAACAACACTATTATGCCCACCA TTTTTTTGAGATGCATCTACACAAGGAACAAACACTGGATGTCACTTTCA GTTCAAATTGTAACGCTAATCACTCCGAACAGGTCACAAAAAATTACCTT AAAAAGTCATAATATTAAATTAGAATAAATATAGCTGTGAGGGAAATATA TACAAATATATTGGAGCAAATAAATTGTACATACAAATATTTATTACTAA TTTCTATTGAGACGAAATGAACCACTCGGAACCATTTGAGCGAACCGAAT CGCGCGGAACTAACGACAGTCGCTCCAAGGTCGTCGAACAAAAGGTGAAT GTGTTGCGGAGAGCGGGTGGGAGACAGCGAAAGAGCAACTACGAAACGTG GTGTGGTGGAGGTGAATTATGAAGAGGGCGCGCGATTTGAAAAGTATGTA TATAAAAAATATATCCCGGTGTTTTATGTAGCGATAAACGAGTTTTTGAT GTAAGGTATGCAGGTGTGTAAGTCTTTTGGTTAGAAGACAAATCCAAAGT CTACTTGTGGGGATGTTCGAAGGGGAAATACTTGTATTCTATAGGTCATA TCTTGTTTTTATTGGCACAAATATAATTACATTAGCTTTTTGAGGGGGCA ATAAACAGTAAACACGATGGTAATAATGGTAAAAAAAAAAAACAAGCAGT TATTTCGGATATATGTCGGCTACTCCTTGCGTCGGGCCCGAAGTCTTAGA GCCAGATATGCGAGCACCCGGAAGCTCACGATGAGAATGGCCAGACCCAC GTAGTCCAGCGGCAGATCGGCGGNCGAGAAAGTAAGCGTCTCCAGAATGA CCTTGCCCGAAACTGGGCACGTGNTGTTCGACGATGTGCAGCTAATTTCG CCCGGCTCACGTCCGCCCATTGGTAATCAGCAGACCCTCGNTNNGCGTAC GAACCCATGAGAGTACGACACCATTTNGAGTAACTGNCACGAATCCGAGT CAGAAGAGCCGCCAAGAGCAGATGTATGATACCNGCGAACCACGACGCTC ATCAGTCAAGACTGCGCAGATTAGAATCGAAGACGTGACCTGGCCACAAT GACACCCCAGCATGAAAATCANCTCGNCCCATCGATCTCGATGCATCCTG AAACCAGGCCTGAAAAACGCAATCCATCTTCTCAAATATGTAGCAAAGAA ATGGCCCTCAAAAGGGCTAGGACCAACAATTTTAAAAAAATTAGAAGGTT ACACTTTTCCCCATTAAGTGTTGAAAAAATTTTATCCCCGTTTTTGAAAA
Vector Information:
| Vector Name: | pWALIUM10-roe |
| Type: | bacterial plasmid |
| Description: | Drosophila expression vector for Recombination cloning, Over-Expression (ROE). loxP, and GATEWAY recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two Gypsy insulators, two 5xUAS, Hsp70 promoter, with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA |
| Properties: | drosophila in vitro and in vivo expression |
| Comments: | To amplify this vector you will need to use One Shot ccdB Survival 2 T1R Competent Cells(Invitrogen Cat. No. A10460), or other gateway competent cells. Revised 4/25/2012 |
| Map: | WALIUM10roe_map_seq.pdf |
| Sequence: | pWALIUM10-roe.txt |
| Size (bp): | 11556 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| DB3.1 T1 phage resistant | N |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
| drosophila | white |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
