Clone: EvNO00422226 ← Click Here To Order

Clone ID:EvNO00422226
Is Verified:N
Status:AVAILABLE
Source:DRSC
Description:Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP sites and restriction sites for EcoRI, BgIII, and Xbal.Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, MCS, with ftz intron and SV40 polyA. Vermilion selection in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Sequencing Information:

Phred Score: 911
Date: 2017-01-23

Last Passing Sequencing Read:

>26584-13_EvNO00422226-T7_E2 911 1430 33 940 NNNCCCCGGCACTGGATATTTTGTATGTATATGTATTATATGTCAGACAT AAAGAAAAGGAACACATCAAATGTGATAACAAAGACTAAACAAGTAATTT TATTACACCAAAACGACAAAACAGTAGGCAGAACAAACAACGCATAGCCA AACATTGACGAATTGGATACCCTGCCGATTGTCAGACACTTTTGTTGATC AGTTTCTTGCGAATGGTCTCGTCCAGCGGTGGAATCGCCTCGCGGGGAAT CAGAAAAGTGGACAGATTGAACAGATCCAGAAACACCTTGTACCGATCAC TGAAACCAAAAAAAAACAAAGGGAGAACAGTTTGAGTTCATTGATCCCCG ATATAATCACATCTGCGATGATCACCTGAGAGTGGAGCGCAGATATTGAT ATCCAGACGAGCCACCAGTGCCCAACTGTTGGGATCCAATCATGCGTTGC ACCATGATCACGTGATTGTCTGCGGCGGGAATAGAAAGTATTTGGTTAGG AAAACCAGTCTTAAACATAAGATATATTTATAAAAGAGTATCAAAGAATG CAATACTTACATCTCCACTTGGTTATTAACGAGTCGATGTCCATGAGCAG GGTGAGCAACTGGTGTGGTTGGCTGAACCTGGGTTCATCCCTATAGAAGG TGATCATGATGGCTCCCTGAAGGGCACGATGGCTAAACCGGCGATCCCCA CGACGCACCAGTGCATCGTGCACTGCCGGATCAAAGATGGAGCGATACAC CTCGCGTCGCTTCTCAATGTCCATGAGGCGGTAGTTTTTCGCCTTCTCCA CGGGCTCCTCCATGGCGCTCTGTACCTGCGCCTCCAGGAATCGATCGACG CTCTCCTNGAACTTGGCCCAGAAGTTGAAGCCACTCTCCTCCAGTCCGGG CGTCCTCTCCAGCCATCGCTGCACTAGCTCCAGTAGCGAGGGATCTTTCT CCGAGTTGCGAATCGAGTTCCGCGCCTCCTCGTCGCTAAAGACATCCGAG TACTTCTGGTTGTATCTCACCCGCTGCTCTGTCAGAACTCCCAGCTTGTT CTCGATCNNAACGAACTGCAGCGACTGAAAACCAGATGCGGGTGCCAGGT ACTTGCGGAAGTCCATGAAGTCTAGCGGGGTCATGGTCTTCAGATGGGCC ACTGGTCACCAGNAGCTGTACAAAGGAAGTAATAACGGATTTTGGTAGAA AATCCGAAGGCCTTCCTTTATAATCGGAACACTCGGTCAGTCGCTGGAAA ATTCCACGTCTTGGTTCCATCAAGACCTCGCTCCAAAGTTCCGAATGAGG CAATCCAAAATATCGTGTTAACCAATCCAAGGCTGGGGAAGACATTAAGC CTTTGGGGGGTCCAAATGTAACCTATCCCCCGGCGGGAGAAAAAAAGTTC

Vector Information:

Vector Name:pVALIUM10-moe
Type:bacterial plasmid
Description:Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP sites and restriction sites for EcoRI, BgIII, and Xbal.Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, MCS, with ftz intron and SV40 polyA. Vermilion selection in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA.
Properties:drosophila in vitro and in vivo expression
Comments:Revised 4/25/2012
Map:VALIUM10moe_map_seq.pdf
Sequence:pVALIUM10-moe.txt
Size (bp):7541
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha N

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
drosophila vermilion

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.