Clone: EvNO00422224
| Clone ID: | EvNO00422224 |
| Is Verified: | N |
| Status: | AVAILABLE |
| Source: | DRSC |
| Description: | Drosophila expression vector for high expression of hairpin insert. loxP, and GATEWAY recombination sites, NheI andEcRI method to clone shmiR. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html P-Trans promoter with ftz intron, two gypsy insulators,two 5xUAS, K10 localization element and vermilion selection in drosophila. AmpR in bacteria; vermilion marker in drosophila. Specialty sequencing primer required to verify insert. Vector may recombine in bacteria and should be stored as DNA. |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
Related Identifiers:
Sequencing Information:
| Phred Score: | 870 |
| Date: | 2017-12-18 |
Last Passing Sequencing Read:
>28181-53_EvNO00422224-5-T3_E7 870 1395 38 976 NNNNAACGGACTCGCGGTGTTGGTTGGCACACCAAAATATACTGTTGCCG AGCACAATTGATCGGCTAAATGGTATGGCAAGAAAAGGTATGCAATATAA TAATCTTTTATTGGGTATGCAACGAAAATTTGTTTCGTCAACGTATGCAA TATTCTTTATTAAAAGAGGGTATGCAATGTATTTTATTAAAAACGGGTAT GCAATATAATAATCTTTTATTGGGTATGCAACGAAAATTTGTTTCGTCAA AGTATGCAATATTTTTTATTAAAAGAGGGTATGCAATGTATTTTATTAAA AACGGGTATGCAATAAAAAATTATTTGGTTTCTCTAAAAAGTATGCAGCA CTTATTTTTTGATAAGGTATGCAACAAAATTTTACTTTGCCGAAAATATG CAATGTTTTTGCGAATAAATTCAACGCACACTTATTACGTGGCCACTGCA GCCAATCCGCCGCACCCTCAGCTCCAAAGCACGCATATGACCCGTGAGCA GGCAGAGAAACGCAGGCGGATAGGAGTCACTAGCGCAGTTCTGTATAGCC TGCACTATCAAGCCGAAGAGCTGGTAGATATTGATGAGCACATAGTTTCT GGTGGAGTGCATCCAGTCAACGCCGAACCAGGCCGGATACAGGAGCTCTC GATCCGGGCGAACGACCACGCGGACGCAACTCAAAGTAGTGCCAAATACG AAAATACTGGCAAAGGTGCGGAAAGTGCCCTGTGCGATATTCACTTCCTT CCTCAAGGCAGAAATCTCCTCGGGATCGCCCACCAGCCGGGCTCTAGCGT CCATGAGCCTTAGGAGAGAGCGAATCTCATGGAGCTGCTTCCTCACCATG TACACATTCGCAAACTTCAAATTGGCCACAATATCGGTAATAGTTATGGT CAGGTTCTCGCACAATCGGGCCATGTTGGTGGTGAACAGCAGCCTGGCCA GCAAGCTCAAGGGAAACAGCACGGTGACCCACCAGTTGACCGTTATGGAG TATACCACGTAAAGCAGGCTTGAAACGCCCGNAGGACGCATCAGGCCAGT GAGCTCCCAAACGCGCCAGTGTTAGTACACAGCACTGTGGGGTGTCAGTT TGAAATCCTCTGCTTCTCCATGTCTCGGTTACCTTTGGGCAATCCAGGGG TTCTATGGCTATAACTCTTGGAATACCAGTGATGCGCTTATACTTATAAT GGATTGTNGCCAAACTGTCGCTAAGTGCCTGGATTGAACCGTAGCCCAGG TAAGATCCCCAAAAAATTTCCAGGCCTTATTTTGGTGCAACCCCTTTTTG GGGATCTTCTTATGGGGAGGGAAGGTTTTAAAACACTTTATTTACCATGC
Vector Information:
| Vector Name: | pVALIUM22 |
| Type: | bacterial plasmid |
| Description: | Drosophila expression vector for high expression of hairpin insert. loxP, and GATEWAY recombination sites, NheI andEcRI method to clone shmiR. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html P-Trans promoter with ftz intron, two gypsy insulators,two 5xUAS, K10 localization element and vermilion selection in drosophila. AmpR in bacteria; vermilion marker in drosophila. Specialty sequencing primer required to verify insert. Vector may recombine in bacteria and should be stored as DNA. |
| Properties: | drosophila in vitro and in vivo expression |
| Comments: | Revised 4/25/2012 |
| Map: | VALIUM22_map_seq.pdf |
| Sequence: | pVALIUM22.txt |
| Size (bp): | 8973 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| EB5-alpha | N |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
| drosophila | vermilion |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
