Clone: EvNO00422222
| Clone ID: | EvNO00422222 |
| Is Verified: | N |
| Status: | AVAILABLE |
| Source: | DRSC |
| Description: | Drosophila expression vector for high expression of hairpin insert. Hsp70 promoter with 5xUAS, two gypsy insulators, and two ftz introns. AmpR in bacteria; white marker in drosophila. loxP and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Vector may recombine in bacteria and should be stored as DNA. |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
| Map: | WALIUM10map.pdf |
Related Identifiers:
Sequencing Information:
| Phred Score: | 852 |
| Date: | 2017-02-15 |
Last Passing Sequencing Read:
>26611-8_EvNO00422222-M13F_H1 852 1340 32 861 NNTACATCGAATCTATAGGCGATTGGGTACAGCTTCACGACCTAGGCGCG CCCTAGTTCCAGTGAAATCCAAGCATTTTCTAAATTAAATGTATTCTTAT TATTATAGTTGTTATTTTTGATATATATAAACAACACTATTATGCCCACC ATTTTTTTGAGATGCATCTACACAAGGAACAAACACTGGATGTCACTTTC AGTTCAAATTGTAACGCTAATCACTCCGAACAGGTCACAAAAAATTACCT TAAAAAGTCATAATATTAAATTAGAATAAATATAGCTGTGAGGGAAATAT ATACAAATATATTGGAGCAAATAAATTGTACATACAAATATTTATTACTA ATTTCTATTGAGACGAAATGAACCACTCGGAACCATTTGAGCGAACCGAA TCGCGCGGAACTAACGACAGTCGCTCCAAGGTCGTCGAACAAAAGGTGAA TGTGTTGCGGAGAGCGGGTGGGAGACAGCGAAAGAGCAACTACGAAACGT GGTGTGGTGGAGGTGAATTATGAAGAGGGCGCGCGATTTGAAAAGTATGT ATATAAAAAATATATCCCGGTGTTTTATGTAGCGATAAACGAGTTTTTGA TGTAAGGTATGCAGGTGTGTAAGTCTTTTGGTTAGAAGACAAATCCAAAG TCTACTTGTGGGGATGTTCGAAGGGGAAATACTTGTATTCTATAGGTCAT ATCTTGTTTTTATTGGCACAAATATAATTACATTAGCTTTTTGAGGGGGC AATAAACAGTAAACACGATGGTAATAATGGTAAAAAAAAAAAACAAGCAG TTATTTCGGATATATGTCGGCTACTCCTTGCGTCGGGCCCGAAGTCTTAG AGCCAGATATGCGAGCACCCGGAAGCTCACGATGAGAATGGCCAGACCCA CGTAGTCCAGCGGCAGATCGGCGGCGGAGAAAGTAAGCGTCTCCAGAATG ACCTTTGCCGAACTGGGGCACGTGGTGTTCGACGATGTGCAGCTAATTTC GCCCNGCTCACGTCCGCCCATTGGNTAATCAGCAGACCCTCGTTTGGCGT ACGAANCCATGAGAGTACGACNACCATTTGAGTATACTGGCACGGAGCCC GAAGTTCAGAAGAAGCCGCCNAAGAAGCAGAATGGTATTGATACCGGCGG ACCCACGACAGCGCCATCNAGNTCAAGGAGCTGNCCCACGAAATTAAAAT CCCAAGGAACTTGACCCATGGCCCCCAAAAGACCACCCCCAGCCATTAAA AAAATGANAACTCGGCCCCCANTCCATATTCGAAAAGGAAATCCCCGAAA
Vector Information:
| Vector Name: | pWALIUM10 |
| Type: | bacterial plasmid |
| Description: | Drosophila expression vector for high expression of hairpin insert. Hsp70 promoter with 5xUAS, two gypsy insulators, and two ftz introns. AmpR in bacteria; white marker in drosophila. loxP and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Vector may recombine in bacteria and should be stored as DNA. |
| Properties: | drosophila in vitro and in vivo expression |
| Comments: | To amplify this vector you will need to use One Shot ccdB Survival 2 T1R Competent Cells(Invitrogen Cat. No. A10460), or other gateway competent cells. Revised 4/25/2012 |
| Map: | WALIUM10_map_seq.pdf |
| Sequence: | pWALIUM10.txt |
| Size (bp): | 13426 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| DB3.1 T1 phage resistant | N |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
| bacterial | chloramphenicol |
| insect cells | white |
