Clone: EvNO00401221 ← Click Here To Order

Clone ID:EvNO00401221
Is Verified:N
Status:AVAILABLE
Source:DRSC
Description:Drosophila expression vector for high expression of hairpin insert MCS, loxP, and attB recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Hsp70 promoter with white and ftz intron. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Sequencing Information:

Phred Score: 930
Date: 2018-05-14

Last Passing Sequencing Read:

>22228-6_EvNO00401221-M13-R_F1 930 1484 29 967 NTTTGGCAGCGCATTACCTCACTAAAGGGACAAAGCTGGAGCTCTGGAAC CGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGC AGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTCCATTCA TTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGT AAAACCTCTACAAATGTGGTATGGCTGATTATGATCTCTAGTCAAGGCAC TATACATCAAATATTTTTCCATAATTTTCTTGTATAGCAGTGCAGCTTTT TCCTTTGTGGTGTAAATAGCAAAGCAAGCAAGAGTTCTATTACTAAACAC AGCATGACTCAAAAAACTTAGCAATTCTGAAGGAAAGTCCTTGGGGTCTT CTACCTTTCTCTTCTTTTTTGGAGGAGTAGAATGTTGAGAGTCAGCAGTA GCCTCATCATCACTAGATGGCATTTCTTCTGAGCAAAACAGGTTTTCCTC ATTAAAGGCATTCCACCACTGCTCCCATTCATCAGTTCCATAGGTTGGAA TCTAAAATACACAAACAATTAGAATCAGTAGTTTAACACATTATACACTT AAAAATTTTATATTTACCTTAGAGCTTTAAATCTCTGTAGGTAGTTTGTC CAATTATGTCACACCACAGAAGTAAGGTTCCTTCACAAAGATCCCGCGGC TCTAGTTCTTTGCAATCTGTAAGCATAAGCAAAGAAAAAATGGGTTAGCT AATGAGTTTTATAACTGCGTATTATTTGAAAATATCAAAAATGTATACAA AAACATATTTTCAATATTTTCAAAGTGTATTTTTAGGGGTTGTTAATCGT GTGTGATGCCTACCTGATGCCAACAATTGTCTAGCAGATCTGCATATGTT CTAGATGCTAGCTGAGTTTCAAATTGGTAATTGGACCCTTCATTAGATTT CACACAGATCAGCCGACTGCGAATAGAAACTCACCCTAGCACTAGTAGCG NCCGCTGAATTCCCAATTCCNTAATCAGATTTCCTCTCTGAATTCATAAT TTACTCNTGNCAGAATTCAGTAGGTGCAGTTGATTTNACTGGNTGCTGGN TACTTNAANNTGATCACTTTAACTTGCCTTNACTGCAGATTGTTNAGCTG NTCAACTGCCCTTGTTTATTGCTAGCTTCCGCTACCAACCGGTCCCTTGC CTGGTTGAATGGAATGCCCCTCCGAAAACAAGCGCCTCATTTAACTCCGG GCTTCCTTCCAAAAATCTCCTTTGAAGAAAAATCCCTCTGAAGAAATACC CCTCCGAGAAATATCCCCTCAGAAAATATTCCCTCGAGAAAATCCCCCCG CGGACACAATTTTATAAAATAACAAAATCTGGGGACTCCCCGAGAAAACC

Vector Information:

Vector Name:pVALIUM1
Type:bacterial plasmid
Description:Drosophila expression vector for high expression of hairpin insert MCS, loxP, and attB recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Hsp70 promoter with white and ftz intron. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA.
Properties:drosophila in vitro and in vivo expression
Comments:Revised 4/25/2012
Map:VALIUM1_map_seq.pdf
Sequence:pVALIUM1.txt
Size (bp):6742
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant N

Antibiotic Selections:

Host Type Marker
drosophila vermilion

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.