Clone ID:	EvNO00401221
Is Verified:	N
Status:	AVAILABLE
Source:	DRSC
Description:	Drosophila expression vector for high expression of hairpin insert MCS, loxP, and attB recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Hsp70 promoter with white and ftz intron. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA.
Comments:
Type:	No insert
Verification Method:
Distribution:	Academic and non-profit labs
Special MTA:

Map:	pVALIUM1.jpg

Related Identifiers:

Property:

Insert Information:

Insert

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Species

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Keyword

Insert Sequence:

Sequencing Information:

Phred Score:	847
Date:	2015-04-07

Sequencing Read:

NNNGGGCAGGCATACCTCCTAAGGGGACAAAAGCTGGAGCTCTGGAACCA GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCA GTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTG TAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTCCATTCAT TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTA AAACCTCTACAAATGTGGTATGGCTGATTATGATCTCTAGTCAAGGCACT ATACATCAAATATTTTTCCATAATTTTCTTGTATAGCAGTGCAGCTTTTT CCTTTGTGGTGTAAATAGCAAAGCAAGCAAGAGTTCTATTACTAAACACA GCATGACTCAAAAAACTTAGCAATTCTGAAGGAAAGTCCTTGGGGTCTTC TACCTTTCTCTTCTTTTTTGGAGGAGTAGAATGTTGAGAGTCAGCAGTAG CCTCATCATCACTAGATGGCATTTCTTCTGAGCAAAACAGGTTTTCCTCA TTAAAGGCATTCCACCACTGCTCCCATTCATCAGTTCCATAGGTTGGAAT CTAAAATACACAAACAATTAGAATCAGTAGTTTAACACATTATACACTTA AAAATTTTATATTTACCTTAGAGCTTTAAATCTCTGTAGGTAGTTTGTCC AATTATGTCACACCACAGAAGTAAGGTTCCTTCACAAAGATCCCGCGGCT CTAGTTCTTTGCAATCTGTAAGCATAAGCAAAGAAAAAATGGGTTAGCTA ATGAGTTTTATAACTGCGTATTATTTGAAAATATCAAAAATGTATACAAA AACATATTTTCAATATTTTCAAAGTGTATTTTTAGGGGTTGTTAATCGTG TGTGATGCCTACCTGATGCCAACAATTGTCTAGCAGATCTGCATATGTTC TAGATGCTAGCTGAGTTTCAAATTTGGAATTGGACCCCTCATTAAGATTT CACACAAATCAGCCGACTGCGAATAGAAACTCACCTAGNCACTAGTAACG GCCGCTGAAATTCCATTTTCTTATCCAAATTTCCCTTCTGAAATCAAAAA ATACTTCTTGCCAGAATTCCAAAATTGCAAGTGATATACTTGGGTGCCGG GTACTTTAAATGAATCACTTTAACCTGCCCTTTATGGCAAATGGTTTACT TGTCCACCTCCCCTTGTTATTGCCTAACCTTTCCTTAAGCCACGGGTACC TTTCCTTGTTTAAAATAAATTGCCCCCCCCAAAAAAAACCCCCCTTTTAT ACCCGGGCCTCCTTGTACAAAAATTCCCCTTGGAAGAAAACTCCCTCTGG AAAAAATATCCTCTTAGAAAAAAAACTCCCCGAGAAAATAACTCCCTCTA

Vector Information:

Vector Name:	pVALIUM1
Type:	bacterial plasmid
Description:	Drosophila expression vector for high expression of hairpin insert MCS, loxP, and attB recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Hsp70 promoter with white and ftz intron. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA.
Properties:	drosophila in vitro and in vivo expression
Comments:	Revised 4/25/2012
Map:	VALIUM1_map_seq.pdf
Sequence:	pVALIUM1.txt
Size (bp):	6742
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
DH5-alpha T1 phage resistant	N

Antibiotic Selections:

Host Type	Marker
drosophila	vermilion

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	100 ug/mL ampicillin	Growth with the single antibiotic in LB at 37 degrees is recommended.	Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

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