Clone ID:	EvNO00401221
Is Verified:	N
Status:	AVAILABLE
Source:	DRSC
Description:	Drosophila expression vector for high expression of hairpin insert MCS, loxP, and attB recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Hsp70 promoter with white and ftz intron. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA.
Comments:
Type:	No insert
Verification Method:
Distribution:	Academic and non-profit labs
Special MTA:

Map:	pVALIUM1.jpg

Related Identifiers:

Sequencing Information:

Phred Score:	930
Date:	2018-05-14

Last Passing Sequencing Read:

>22228-6_EvNO00401221-M13-R_F1 930 1484 29 967 NTTTGGCAGCGCATTACCTCACTAAAGGGACAAAGCTGGAGCTCTGGAAC CGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGC AGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTCCATTCA TTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGT AAAACCTCTACAAATGTGGTATGGCTGATTATGATCTCTAGTCAAGGCAC TATACATCAAATATTTTTCCATAATTTTCTTGTATAGCAGTGCAGCTTTT TCCTTTGTGGTGTAAATAGCAAAGCAAGCAAGAGTTCTATTACTAAACAC AGCATGACTCAAAAAACTTAGCAATTCTGAAGGAAAGTCCTTGGGGTCTT CTACCTTTCTCTTCTTTTTTGGAGGAGTAGAATGTTGAGAGTCAGCAGTA GCCTCATCATCACTAGATGGCATTTCTTCTGAGCAAAACAGGTTTTCCTC ATTAAAGGCATTCCACCACTGCTCCCATTCATCAGTTCCATAGGTTGGAA TCTAAAATACACAAACAATTAGAATCAGTAGTTTAACACATTATACACTT AAAAATTTTATATTTACCTTAGAGCTTTAAATCTCTGTAGGTAGTTTGTC CAATTATGTCACACCACAGAAGTAAGGTTCCTTCACAAAGATCCCGCGGC TCTAGTTCTTTGCAATCTGTAAGCATAAGCAAAGAAAAAATGGGTTAGCT AATGAGTTTTATAACTGCGTATTATTTGAAAATATCAAAAATGTATACAA AAACATATTTTCAATATTTTCAAAGTGTATTTTTAGGGGTTGTTAATCGT GTGTGATGCCTACCTGATGCCAACAATTGTCTAGCAGATCTGCATATGTT CTAGATGCTAGCTGAGTTTCAAATTGGTAATTGGACCCTTCATTAGATTT CACACAGATCAGCCGACTGCGAATAGAAACTCACCCTAGCACTAGTAGCG NCCGCTGAATTCCCAATTCCNTAATCAGATTTCCTCTCTGAATTCATAAT TTACTCNTGNCAGAATTCAGTAGGTGCAGTTGATTTNACTGGNTGCTGGN TACTTNAANNTGATCACTTTAACTTGCCTTNACTGCAGATTGTTNAGCTG NTCAACTGCCCTTGTTTATTGCTAGCTTCCGCTACCAACCGGTCCCTTGC CTGGTTGAATGGAATGCCCCTCCGAAAACAAGCGCCTCATTTAACTCCGG GCTTCCTTCCAAAAATCTCCTTTGAAGAAAAATCCCTCTGAAGAAATACC CCTCCGAGAAATATCCCCTCAGAAAATATTCCCTCGAGAAAATCCCCCCG CGGACACAATTTTATAAAATAACAAAATCTGGGGACTCCCCGAGAAAACC

Vector Information:

Vector Name:	pVALIUM1
Type:	bacterial plasmid
Description:	Drosophila expression vector for high expression of hairpin insert MCS, loxP, and attB recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Hsp70 promoter with white and ftz intron. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA.
Properties:	drosophila in vitro and in vivo expression
Comments:	Revised 4/25/2012
Map:	VALIUM1_map_seq.pdf
Sequence:	pVALIUM1.txt
Size (bp):	6742
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
DH5-alpha T1 phage resistant	N

Antibiotic Selections:

Host Type	Marker
drosophila	vermilion

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	100 ug/mL ampicillin	Growth with the single antibiotic in LB at 37 degrees is recommended.	Commonly used conditions for ampicillin resistant plasmid clones.