Clone ID:	EvNO00401203
Is Verified:	N
Status:	AVAILABLE
Source:
Description:	Mammalian expression vector for use in lentiviral work. LoxP site cloning. Fusion format inserts will be expressed with HA-Flag tag that can be systematically removed during protein purification with TEV and EK cleavage. Viral components on vector improve expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines.
Comments:
Type:	No insert
Verification Method:	end read sequencing with CMV-F
Distribution:	Academic and non-profit labs
Special MTA:

Map:	pCW63-T7-Tag.pdf

Related Identifiers:

Sequencing Information:

Phred Score:	857
Date:	2015-06-11

Last Passing Sequencing Read:

CNNGGGGCATTNCAGAGCNGTTAGTGAACCGGTCAGATCGCCTGGAGAATTGGCTAGCGGCATAATACGACTCACTATAG GGTTTAAACTTAATTAAGCACCATGTACCCCTACGACGTGCCCGACTACGCCGAAAACCTATACTTCCAATCACTGCAGG ACTACAAGGACGACGATGACAAGGTCGACATAACTTCGTATAGCATACATTATACGAAGTTATAGATCCAATATTATTGA AGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG CACATTTCCCCGAAAAGTGCCACCTGACGTGGATCTCGAGCTCAAGCTTCGAATTCAGGGTTTCCTTGACAATATCATAC TTATCCTGTCCCTTTTTTTTCCACAGCTACCGGTCCACCACCACCACCACCACTAACCCGGGGGATCCGGGGTTGGGGTT GCGCCTTTTCCAAGGCAGCCCTGGGTTTGCGCAGGGACGCGGCTGCTCTGGGCGTGGTTCCGGGAAACGCAGCGGCGCCG ACCCTGGGTCTCGCACATTCTTCACGTCCGTTCGCAGCGTCACCCGGATCTTCGCCGCTACCCTTGTGGGCCCCCCGGCG ACGCTTCCTGCTCCGCCCCTAAGTCGGGAAGGTTCCTTGCGGTTCGCGGCGTGCCGGACGTGACAAACGGAAGCCGCACG TCTCACTAGTACCCTCGCAGACGGACAGCGCCAGGGAGCAATGGCAGCGCGCCGACCGCGATGGGCTGTGGCCAATAGCG GCTGCTCAGCAGGGCGCGCCGAGAGCAGCGGCCGGNAAGGGGCGGTGCGGGAGGCGGGGTGTGGGGCGGTAGTGTGGGCC CTGTTCCTGCCCGCGCGGTGTTCCGCATTCTGCAAGCCTCCGGAGCGCANGTCCGGCAGNTCGGCTCCCTCGTTGACCGA AATCACCGACCTCTNTCCCCCNGCAATTCNCCNTGGTCTANACTGGNACAANAAGCAAAGTCNNTAAACGGAACCTCTGG GAATTACTNCAANGGGGGNCGGGNATCNAAGGCCTGGACNACAAGGAAAACTCNCNTCAAAANGCTGGGAAGNTGGACNA CCCTACCCTNGTTCTGGNCNNNGGAANAANNANGNGGGCCCNGGNCNCNATGCCCNTGGCCAANNCGNGANGGNTGGAAN GGNNTTATANCCCNCTTTTGGCCCCTGGAAGGGAANTTTGGGNNAAAAANTTTTGGGGAAAAACNCCNAGTNTTNCNNCT TGGGTCCCNTNCCATNGGGGGGGNCTAANNNNNCCCCGCCCCCCCCAAAAAAAAACGTNAAAACCCGGAAACNTNNGNTT NTGGNNAAAGGTTNCCNTGAAAAAANNNNNCCTTNCCCNGGGCCNTTNNGGGGGGNTGGGNAAGGNNCNNTNAAAAAAAA AAACCCCCCTTTTCCCCTTAAAAANTNTTCCCGGG

Vector Information:

Vector Name:	pCW63
Type:	bacterial plasmid
Description:	Mammalian expression vector for use in lentiviral work. LoxP site cloning. Fusion format inserts will be expressed with HA-Flag tag that can be systematically removed during protein purification with TEV and EK cleavage. Viral components on vector improve expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines.
Properties:	mammalian expression
Comments:
Map:	pCW63-T7-Tag.pdf
Sequence:	pCW63.txt
Size (bp):	7520
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
DH5-alpha T1 phage resistant	N

Antibiotic Selections:

Host Type	Marker
bacterial	ampicillin
mammalian	blasticidin

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	100 ug/mL ampicillin	Growth with the single antibiotic in LB at 37 degrees is recommended.	Commonly used conditions for ampicillin resistant plasmid clones.