Clone: EvNO00401203
Clone ID: | EvNO00401203 |
Is Verified: | N |
Status: | AVAILABLE |
Source: | |
Description: | Mammalian expression vector for use in lentiviral work. LoxP site cloning. Fusion format inserts will be expressed with HA-Flag tag that can be systematically removed during protein purification with TEV and EK cleavage. Viral components on vector improve expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines. |
Comments: | |
Type: | No insert |
Verification Method: | end read sequencing with CMV-F |
Distribution: | Academic and non-profit labs |
Special MTA: |
Map: | pCW63-T7-Tag.pdf |
Related Identifiers:
Sequencing Information:
Phred Score: | 857 |
Date: | 2015-06-11 |
Last Passing Sequencing Read:
CNNGGGGCATTNCAGAGCNGTTAGTGAACCGGTCAGATCGCCTGGAGAATTGGCTAGCGGCATAATACGACTCACTATAG GGTTTAAACTTAATTAAGCACCATGTACCCCTACGACGTGCCCGACTACGCCGAAAACCTATACTTCCAATCACTGCAGG ACTACAAGGACGACGATGACAAGGTCGACATAACTTCGTATAGCATACATTATACGAAGTTATAGATCCAATATTATTGA AGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG CACATTTCCCCGAAAAGTGCCACCTGACGTGGATCTCGAGCTCAAGCTTCGAATTCAGGGTTTCCTTGACAATATCATAC TTATCCTGTCCCTTTTTTTTCCACAGCTACCGGTCCACCACCACCACCACCACTAACCCGGGGGATCCGGGGTTGGGGTT GCGCCTTTTCCAAGGCAGCCCTGGGTTTGCGCAGGGACGCGGCTGCTCTGGGCGTGGTTCCGGGAAACGCAGCGGCGCCG ACCCTGGGTCTCGCACATTCTTCACGTCCGTTCGCAGCGTCACCCGGATCTTCGCCGCTACCCTTGTGGGCCCCCCGGCG ACGCTTCCTGCTCCGCCCCTAAGTCGGGAAGGTTCCTTGCGGTTCGCGGCGTGCCGGACGTGACAAACGGAAGCCGCACG TCTCACTAGTACCCTCGCAGACGGACAGCGCCAGGGAGCAATGGCAGCGCGCCGACCGCGATGGGCTGTGGCCAATAGCG GCTGCTCAGCAGGGCGCGCCGAGAGCAGCGGCCGGNAAGGGGCGGTGCGGGAGGCGGGGTGTGGGGCGGTAGTGTGGGCC CTGTTCCTGCCCGCGCGGTGTTCCGCATTCTGCAAGCCTCCGGAGCGCANGTCCGGCAGNTCGGCTCCCTCGTTGACCGA AATCACCGACCTCTNTCCCCCNGCAATTCNCCNTGGTCTANACTGGNACAANAAGCAAAGTCNNTAAACGGAACCTCTGG GAATTACTNCAANGGGGGNCGGGNATCNAAGGCCTGGACNACAAGGAAAACTCNCNTCAAAANGCTGGGAAGNTGGACNA CCCTACCCTNGTTCTGGNCNNNGGAANAANNANGNGGGCCCNGGNCNCNATGCCCNTGGCCAANNCGNGANGGNTGGAAN GGNNTTATANCCCNCTTTTGGCCCCTGGAAGGGAANTTTGGGNNAAAAANTTTTGGGGAAAAACNCCNAGTNTTNCNNCT TGGGTCCCNTNCCATNGGGGGGGNCTAANNNNNCCCCGCCCCCCCCAAAAAAAAACGTNAAAACCCGGAAACNTNNGNTT NTGGNNAAAGGTTNCCNTGAAAAAANNNNNCCTTNCCCNGGGCCNTTNNGGGGGGNTGGGNAAGGNNCNNTNAAAAAAAA AAACCCCCCTTTTCCCCTTAAAAANTNTTCCCGGG
Vector Information:
Vector Name: | pCW63 |
Type: | bacterial plasmid |
Description: | Mammalian expression vector for use in lentiviral work. LoxP site cloning. Fusion format inserts will be expressed with HA-Flag tag that can be systematically removed during protein purification with TEV and EK cleavage. Viral components on vector improve expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines. |
Properties: | mammalian expression |
Comments: | |
Map: | pCW63-T7-Tag.pdf |
Sequence: | pCW63.txt |
Size (bp): | 7520 |
Form: | dsDNA |
Host Information:
Host Strain | Is Used In Distribution | Description |
DH5-alpha T1 phage resistant | N |
Antibiotic Selections:
Host Type | Marker |
bacterial | ampicillin |
mammalian | blasticidin |
Recommended Growth Condition:
Host Type | Selection Condition | Growth Condition | Comments |
bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |