Clone: EvNO00401201
| Clone ID: | EvNO00401201 |
| Is Verified: | Y |
| Status: | AVAILABLE |
| Source: | |
| Description: | Mammalian expression vector for lentiviral work. Fusion format inserts are expressed with self excising fluorescent marker mRFP. LoxP cloning system. Viral components on backbone help expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines. |
| Comments: | |
| Type: | No insert |
| Verification Method: | end read sequencing with CMV-F |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
Related Identifiers:
Sequencing Information:
| Phred Score: | 863 |
| Date: | 2015-06-11 |
Last Passing Sequencing Read:
CNGGGNCNTAGCAGACTGGTTAGTGACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACA CCGGGACCGATCCAGCCTCCGGCTAGCATAACTTCGTATAGCATACATTATACGAAGTTATAGATCCAATATTATTGAAG CATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCA CATTTCCCCGAAAAGTGCCACCTGACGTGGATCTCGAGCTCAAGCTTCGAATTCAGGGTTTCCTTGACAATATCATACTT ATCCTGTCCCTTTTTTTTCCACAGCTACCGGTCCACCACCACCACCACCACTAAGGATCCGCGGCCGCAAATTCCGCCCC TCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTC CACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTT CCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACA ACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATA AGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCT CAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACA TGCTTTACATGTGTTTAGTCGAGGTNAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACA CGATGATAAGCTTGCCACANCATGGCCANNCTTTGTCTCAAGAAAATCCCCCTCATGGAAAGAGCACGGNTAAATCAANA GCATCCCATNTCTGAAANTANAGNGTCNCCNGGCANCTTTTCTAGGGACGGCGCATCTNCANGGGGNCAANGANATCATT TNACNGGGGAACTTGGNNAAANTCNGGGGNTGGGACTGNTGNTGNTGNGNAANTGGAANTNGANTGTATNNCCNNATCGA AAGAAAAAGGGGNNTTNGANCCCNGGGGANGGGCCNAAGGGGNTNCNNATTGNNCNGGGGTNAACCCNNNGANGANNNNN GGNNCCCCNNNNNNGGGATNNNAANNNCCCCCCNNNTNNGGGGGGNAAAAAANNNCCNNNATTTTNNCNTTANNNGGGGA NNNANANCCNGGCCCCNNNGGGGGGNTTTTNNANNAAANNNAAAAANCCCCCCCCCCNNTTTTNTTNNNGGGGGNNNNNN NNNNNNNNGGGGNNNCCCCCCCCCCCCCCCCCCCCCN
Vector Information:
| Vector Name: | pWE-p2A-mRFP |
| Type: | bacterial plasmid |
| Description: | Mammalian expression vector for lentiviral work. Fusion format inserts are expressed with self excising fluorescent marker mRFP. LoxP cloning system. Viral components on backbone help expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines. |
| Properties: | mammalian expression |
| Comments: | |
| Map: | pCW45-2A-mRFP.pdf |
| Sequence: | pCW45-2A-mRFP.txt |
| Size (bp): | 8144 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| DH5-alpha T1 phage resistant | N |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
| mammalian | blasticidin |
