Clone: HsCD00378799

Clone ID:HsCD00378799
Is Verified:Y
Status:AVAILABLE
Source:The ORFeome Collaboration
Description:
Comments:
Type:cDNA
Verification Method:Sequence Verification
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

Broad Clone ID ccsbBroadEn_05264
Original Clone ID 71752

Property:

Collection: CCSB 51a
Collection: Center for Cancer Systems Biology
Collection: The ORFeome Collaboration

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 1743 Homo sapiens FUSION DNA template from MGC 221692 PHACTR1 Homo sapiens phosphatase and actin regulator 1, mRNA (cDNA clone MGC:126575 IMAGE:8069032), complete cds.
Insert1
Size (bp)1743
SpeciesHomo sapiens
FormatFUSION
Tissue SourceDNA template from MGC
Species Specific ID 221692
Gene SymbolPHACTR1
Gene NameHomo sapiens phosphatase and actin regulator 1, mRNA (cDNA clone MGC:126575 IMAGE:8069032), complete cds.
Keyword

Insert Sequence:

ATGGATTATCCCAAAATGGATTATTTTCTGGATGTAGAGTCTGCTCACAGACTCTTGGATGTTGAGTCAGCTCAAAGATTCTTCTACAGTCAAGGAGCTCAAGCTCGCCGGGCGACCCTGCTCCTGCCTCCCACATTAATGGCGGCATCCTCGGAGGATGATATAGACCGGCGGCCCATCCGGAGAGTGCGCTCCAAGAGCGACACGCCGTACCTCGCAGAGGCCAGGATCTCCTTTAACCTGGGGGCAGCTGAGGAAGTGGAGAGGCTGGCGGCGATGCGTTCTGACTCCCTCGTCCCAGGCACCCACACCCCACCCATCCGCAGGAGAAGTAAGTTTGCCAACCTGGGAAGGATTTTCAAGCCTTGGAAATGGAGGAAGAAGAAAAGCGAAAAGTTCAAACACACGTCAGCAGCCCTGGAAAGGAAAATATCTATGAGGCAAAGCAGAGAAGAGCTGATAAAGCGAGGAGTCCTGAAGGAAATCTATGATAAAGATGGGGAACTCTCTATATCCAATGAAGAGGACTCCCTAGAAAATGGGCAGTCCCTGAGCTCCAGCCAGCTGTCTCTGCCTGCCCTGTCCGAAATGGAGCCAGTCCCAATGCCCAGGGATCCCTGCTCATATGAGGTGCTCCAACCGTCAGACATCATGGATGGGCCAGATCCTGGCGCCCCTGTGAAATTGCCTTGTCTGCCAGTGAAACTGTCGCCTCCGCTACCTCCAAAGAAAGTCATGATCTGTATGCCCGTGGGGGGGCCAGACCTCTCACTGGTGTCCTACACAGCCCAGAAGAGTGGCCAGCAGGGTGTGGCCCAGCACCACCACACTGTCCTGCCCTCCCAGATCCAGCACCAGCTGCAGTACGGCAGCCACGGCCAGCACCTCCCCTCCACCACCGGCTCCCTCCCCATGCACCCCTCGGGCTGCAGAATGATAGACGAGCTCAACAAAACGCTGGCCATGACCATGCAGAGGCTGGAAAGCTCTGAGCAGCGGGTCCCCTGTTCCACTTCTTACCACAGCTCTGGGTTGCACTCGGGTGATGGGGTCACCAAAGCAGGACCTATGGGCCTTCCAGAAATAAGACAAGTGCCAACTGTTGTGATTGAATGTGATGACAATAAAGAAAATGTGCCTCATGAGTCAGACTACGAAGACTCTTCTTGCCTGTATACAAGAGAAGAGGAGGAAGAGGAGGAGGACGAAGACGACGACAGCTCATTATACACCAGCTCCCTGGCCATGAAGGTCTGCAGGAAGGACTCCTTAGCCATCAAACTCAGCAACAGGCCCTCCAAGCGAGAGCTGGAAGAAAAGAACATCCTTCCCAGGCAGACGGATGAGGAGCGGCTGGAGCTGAGGCAACAGATTGGCACCAAGCTCACCAGGCGGCTGAGCCAGAGGCCAACTGCAGAGGAACTGGAACAGAGGAACATTTTGAAACCTCGGAATGAACAAGAGGAACAGGAGGAGAAGAGAGAGATCAAGAGGAGGCTAACCCGAAAGCTCAGTCAAAGGCCCACGGTGGAAGAGCTTCGGGAAAGAAAGATCCTCATCCGCTTCAGTGACTACGTGGAGGTGGCTGACGCTCAGGACTATGACCGCAGGGCAGATAAGCCGTGGACCCGCCTCACCGCTGCAGACAAAGCTGCCATCCGAAAGGAGCTCAATGAATTCAAAAGCACTGAGATGGAAGTTCATGAATTGAGTAGACACTTAACAAGGTTTCACCGACCTTTG

Insert Property: Insert 1

Type Value Extra Information
CDS Start 1
CDS Stop 1743
End on reference sequence 1839
Start on reference sequence 97

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCACCATGGATTATCCCAAAATGGATTATTTTCTGGATGTAGAGTCTGCTCACAGACTCTTGGATGTTGAGTCAGCTCAAAGATTCTTCTACAGTCAAGGAGCTCAAGCTCGCCGGGCGACCCTGCTCCTGCCTCCCACATTAATGGCGGCATCCTCGGAGGATGATATAGACCGGCGGCCCATCCGGAGAGTGCGCTCCAAGAGCGACACGCCGTACCTCGCAGAGGCCAGGATCTCCTTTAACCTGGGGGCAGCTGAGGAAGTGGAGAGGCTGGCGGCGATGCGTTCTGACTCCCTCGTCCCAGGCACCCACACCCCACCCATCCGCAGGAGAAGTAAGTTTGCCAACCTGGGAAGGATTTTCAAGCCTTGGAAATGGAGGAAGAAGAAAAGCGAAAAGTTCAAACACACGTCAGCAGCCCTGGAAAGGAAAATATCTATGAGGCAAAGCAGAGAAGAGCTGATAAAGCGAGGAGTCCTGAAGGAAATCTATGATAAAGATGGGGAACTCTCTATATCCAATGAAGAGGACTCCCTAGAAAATGGGCAGTCCCTGAGCTCCAGCCAGCTGTCTCTGCCTGCCCTGTCCGAAATGGAGCCAGTCCCAATGCCCAGGGATCCCTGCTCATATGAGGTGCTCCAACCGTCAGACATCATGGATGGGCCAGATCCTGGCGCCCCTGTGAAATTGCCTTGTCTGCCAGTGAAACTGTCGCCTCCGCTACCTCCAAAGAAAGTCATGATCTGTATGCCCGTGGGGGGGCCAGACCTCTCACTGGTGTCCTACACAGCCCAGAAGAGTGGCCAGCAGGGTGTGGCCCAGCACCACCACACTGTCCTGCCCTCCCAGATCCAGCACCAGCTGCAGTACGGCAGCCACGGCCAGCACCTCCCCTCCACCACCGGCTCCCTCCCCATGCACCCCTCGGGCTGCAGAATGATAGACGAGCTCAACAAAACGCTGGCCATGACCATGCAGAGGCTGGAAAGCTCTGAGCAGCGGGTCCCCTGTTCCACTTCTTACCACAGCTCTGGGTTGCACTCGGGTGATGGGGTCACCAAAGCAGGACCTATGGGCCTTCCAGAAATAAGACAAGTGCCAACTGTTGTGATTGAATGTGATGACAATAAAGAAAATGTGCCTCATGAGTCAGACTACGAAGACTCTTCTTGCCTGTATACAAGAGAAGAGGAGGAAGAGGAGGAGGACGAAGACGACGACAGCTCATTATACACCAGCTCCCTGGCCATGAAGGTCTGCAGGAAGGACTCCTTAGCCATCAAACTCAGCAACAGGCCCTCCAAGCGAGAGCTGGAAGAAAAGAACATCCTTCCCAGGCAGACGGATGAGGAGCGGCTGGAGCTGAGGCAACAGATTGGCACCAAGCTCACCAGGCGGCTGAGCCAGAGGCCAACTGCAGAGGAACTGGAACAGAGGAACATTTTGAAACCTCGGAATGAACAAGAGGAACAGGAGGAGAAGAGAGAGATCAAGAGGAGGCTAACCCGAAAGCTCAGTCAAAGGCCCACGGTGGAAGAGCTTCGGGAAAGAAAGATCCTCATCCGCTTCAGTGACTACGTGGAGGTGGCTGACGCTCAGGACTATGACCGCAGGGCAGATAAGCCGTGGACCCGCCTCACCGCTGCAGACAAAGCTGCCATCCGAAAGGAGCTCAATGAATTCAAAAGCACTGAGATGGAAGTTCATGAATTGAGTAGACACTTAACAAGGTTTCACCGACCTTTGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Sequencing Information:

Phred Score: 877
Date: 2015-07-02

Last Passing Sequencing Read:

NNCCGCCGCTAAGATTTATTTTGACGATATGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCACCATGGATTATCCCAAAATGGATTATTTTCTGGATGTAGAGTCTGCTCACAGACTCTTGGATGTTGAGTCAGCTCAAAGATTCTTCTACAGTCAAGGAGCTCAAGCTCGCCGGGCGACCCTGCTCCTGCCTCCCACATTAATGGCGGCATCCTCGGAGGATGATATAGACCGGCGGCCCATCCGGAGAGTGCGCTCCAAGAGCGACACGCCGTACCTCGCAGAGGCCAGGATCTCCTTTAACCTGGGGGCAGCTGAGGAAGTGGAGAGGCTGGCGGCGATGCGTTCTGACTCCCTCGTCCCAGGCACCCACACCCCACCCATCCGCAGGAGAAGTAAGTTTGCCAACCTGGGAAGGATTTTCAAGCCTTGGAAATGGAGGAAGAAGAAAAGCGAAAAGTTCAAACACACGTCAGCAGCCCTGGAAAGGAAAATATCTATGAGGCAAAGCAGAGAAGAGCTGATAAAGCGAGGAGTCCTGAAGGAAATCTATGATAAAGATGGGGAACTCTCTATATCCAATGAAGAGGACTCCCTAGAAAATGGGCAGTCCCTGAGCTCCAGCCAGCTGTCTCTGCCTGCCCTGTCCGANATGGAGCCAGTCCCAATGCCCAGGGATCCCTGCTCATATGAGGTGCTCCAACCGTCAGACATCATGGATGGGCCAGATCCTGGCGCCCCTGTGAAATTGCCTTGTCTGCCAGTGAAACTGTCGCCTCCGCTACCTCCAAAGAAAGTCATGATCTGTATGCCCGTGGGGGGGCCAGACCTCTCACTGGTGTCCTACACAGCCCAGAAGAGTGGCCAGCAGGGTGTGGCCCAGCACCACAACACTGTCCTGCCCTCCCAGATCCAGCACCAGCTGCAGTACGGGAGCCACGGGCAGAACCTCCCCTTCACAACGGGTTTCCTCCCCATGCACCCCTCGGGCTGCAGAATGATAAACAAGCTCAACAAAACGCTGGCCATGACCATGCAGAAGCTGGAAAGCTTCTGACAAGCGGTTCCCCGTTTCCCCTCCTTACCCAACCTCTGGGTTGCCTTCGGGTGATGGGGTCCCCAAACAGGGACCCATGGGCCTTTCCAAAATAAAAAAAAGGCCAACTGTTTTGATTGAATGTGAAGACAAAAAAGAAAATTGGCCTCATGAACTAAAATAAGAAAACTTTTTTGCTGTTTTCCAAAAAAGGGGGGAAGAAGGTGGGCCCAAACCCACCCCCCTCTTTATACCCCCTCCCGGCCTATAAGTTTCCGAGAGAACTCTTTACCTTAAAATCCCAAAGGCCCCAAGGGAAGTTGAG

Vector Information:

Vector Name:pENTR223
Type:bacterial plasmid
Description:Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Properties:recombinational cloning
Comments:Additional information about this vector is available from the commercial website and at the ORFeome website http://www.orfeomecollaboration.org/html/vectors.shtml; also see supplemental figure 1 of the paper PubMed ID 15489335.
Map:pDONR223.1.gif
Sequence:pENTR223-seq.doc
Size (bp):2815
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial spectinomycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL spectinomycin Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Authors:

Author Name Author Type
The ORFeome Collaboration Consortium
Center for Cancer Systems Biology Clone donor

Publications:

PMID Title
17207965 hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.
154893350 Human ORFeome version 1.1: a platform for reverse proteomics.