Clone: HsCD00333682

Clone ID:HsCD00333682
Is Verified:Y
Status:AVAILABLE
Source:MGC
Description:
Comments: WARNING!!! Frameshift in CDS
Type:cDNA
Verification Method:Sequence Verification
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

IMAGE ID 4827771
GI 28278135
GenBank Accession BC045539
Original Clone ID MGC:57162

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 2632 Homo sapiens CLOSED 22900 CARD8 caspase recruitment domain family, member 8
Insert1
Size (bp)2632
SpeciesHomo sapiens
FormatCLOSED
Tissue Source
Species Specific ID 22900
Gene SymbolCARD8
Gene Namecaspase recruitment domain family, member 8
Keyword

Insert Sequence:

AGAGTAGCCTGGCAGTGTCCAAGCAACAAGCCTCCGCTCCCCCTTCCTGCACCCTGGGGCTCCTGAAACTCACATGAGAAGGAGGGCTGTCTGAGATTCGAGGGAAACAAGCTCTCAGGACTTCCGGTCGCCATGATGGCTGTGGGCGGTAAACGCGGTTAGTGCAAGCATCTGGGCCATCTTCAATGGTAAAAAAGATACAGTAAAGACATAAATACCACATTTGACAAATGGAAAAAAAGGAGTGTCCAGAAAAGAGTAGCAGCAGTGAGGAAGAGCTGCCGAGACGGGTATACAGGGAGCTACCCTGTGTTTCTGAGACCCTTTGTGGCATCTCACATTTTTTCCAAGAAGATGATGAGACAGAGGCAGAGCCATTATTGTTCCGTGCTGTTCCTGATTGTCAACTATCTGGGGGGGACATTCCCAGTGTATCAGAAGAGCAGGAATCTTCAGAGGGACAAGATTCAGGAGACATTTGCTCAGAAGAGAATCAAATAGTTTCCTCTTATGCTTCTAAAGTCTGTTTTGAGATCGAAGAAGATTATAAAAATCGTCAGTTTCTGGGGCCTGAAGGAAATGTGGATGTTGAGTTGATTGATAAGAGCACAAACAGATACAGCGTTTGGTTCCCCACTGCTGGCTGGTATCTGTGGTCAGCCACAGGCCTCGGCTTCCTGGTAAGGGATGAGGTCACAGTGACGATTGCGTTTGGTTCCTGGAGTCAGCACCTGGCCCTGGACCTGCAGCACCATGAACAGTGGCTGGTGGGCGGCCCCTTGTTTGATGTCACTGCAGAGCCAGAGGAGGCTGTCGCCGAAATCCACCTCCCCCACTTCATCTCCCTCCAAGCAGGTGAGGTGGACGTCTCCTGGTTTCTCGTTGCCCATTTTAAGAATGAAGGGATGGTCCTGGAGCATCCAGCCCGGGTGGAGCCTTTCTATGCTGTCCTGGAAAGCCCCAGCTTCTCTCTGATGGGCATCCTGCTGCGGATCGCCAGTGGGACTCGCCTCTCCATCCCCATCACTTCCAACACATTGATCTATTATCACCCCCACCCCGAAGATATTAAGTTCCACTTGTACCTTGTCCCCAGCGACGCCTTGCTAACAAAGGCGATAGATGATGAGGAAGATCGCTTCCATGGTGTGCGCCTGCAGACTTCGCCCCCAATGGAACCCCTGAACTTTGGTTCCAGTTATATTGTGTCTAATTCTGCTAACCTGAAAGTAATGCCCAAGGAGTTGAAATTGTCCTACAGGAGCCCTGGAGAAATTCAGCACTTCTCAAAATTCTATGCTGGGCAGATGAAGGAACCCATTCAACTTGAGATTACTGAAAAAAGACATGGGACTTTGGTGTGGGATACTGAGGTGAAGCCAGTGGATCTCCAGCTTGTAGCTGCATCAGCCCCTCCTCCTTTCTCAGGTGCAGCCTTTGTGAAGGAGAACCACCGGCAACTCCAAGCCAGGATGGGGGACCTGAAAGGGGTGCTCGATGATCTCCAAGACAATGAGGTTCTTACTGAGAATGAGAAGGAGCTGGTGGAGCAGGAAAAGACACGGCAGAGCAAGAATGAGGCCTTGCTGAGCATGGTGGAGAAGAAAGGGGACCTGGCCCTGGACGTGCTCTTCAGAAGCATTAGTGGAAGGGACCCTTACCTCGTGTCCTATCTTAGACAGCAGAATTTGTAAAATGAGTCAGTTAGGTAGTCTGGAAGAGAGAATCCAGCGTTCTCATTGGAAATGGATAAACAGAAATGTGATCATTGATTTCAGTGTTCAAGACAGAAGAAGACTGGGTAACATCTATCACACAGGCTTTCAGGACAGACTTGTAACCTGGCATGTACCTATTGACTGTATCCTCATGCATTTTCCTCAAGAATGTCTGAAGAAGGTAGTAATATTCCTTTTAAATTTTTTCCAACCATTGCTTGATATATCACTATTTTATCCATTGACATGATTCTTGAAGACCCAGGATAAAGGACATCCGGATAGGTGTGTTTATGAAGGATGGGGCCTGGAAAGGCAACTTTTCCTGATTAATGTGAAAAATAATTCCTATGGACACTCCGTTTGAAGTATCACCTTCTCATAACTAAAAGCAGAAAAGCTAACAAAAGCTTCTCAGCTGAGGACACTCAAGGCATACATGATGACAGTCTTTTTTTTTTTTGTATGTTAGGACTTTAACACTTTATCTATGGCTACTGTTATTAGAACAATGTAAATGTATTTGCTGAAAGAGAGCACAAAAATGGGAGAAAATGCAAACATGAGCAGAAAATATTTTCCCACTGGTGTGTAGCCTGCTACAAGGAGTTGTTGGGTTAAATGTTCATGGTCAACTCCAAGGAATACTGAGATGAAATGTGGTAAATCAACTCCACAGAACCACCAAAAAGAAAATGAGGGTAATTCAGCTTATTCTGAGACAGACATTCCTGGCAATGTACCATACAAAAAATAAGCCAACTCTGACATTTGGATTCTACCATAGACTCTGTCATTTTGTAGCCATTTCAGCTGTCTTTTGATTAATGTTTTCGTGGCACACATATTTCCATCCTTTTATGTTTAATCTGTTTAAAACAAGTTCCTAGTAGACACCAAAAAAAAAAAAAAAA

Insert Property: Insert 1

Type Value Extra Information
CDS Start 231
CDS Stop 1694

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

GCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGGCGAATTGGAGCTCCACCGCGGTGGCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGAGTAGCCTGGCAGTGTCCAAGCAACAAGCCTCCGCTCCCCCTTCCTGCACCCTGGGGCTCCTGAAACTCACATGAGAAGGAGGGCTGTCTGAGATTCGAGGGAAACAAGCTCTCAGGACTTCCGGTCGCCATGATGGCTGTGGGCGGTAAACGCGGTTAGTGCAAGCATCTGGGCCATCTTCAATGGTAAAAAAGATACAGTAAAGACATAAATACCACATTTGACAAATGGAAAAAAAGGAGTGTCCAGAAAAGAGTAGCAGCAGTGAGGAAGAGCTGCCGAGACGGGTATACAGGGAGCTACCCTGTGTTTCTGAGACCCTTTGTGGCATCTCACATTTTTTCCAAGAAGATGATGAGACAGAGGCAGAGCCATTATTGTTCCGTGCTGTTCCTGATTGTCAACTATCTGGGGGGGACATTCCCAGTGTATCAGAAGAGCAGGAATCTTCAGAGGGACAAGATTCAGGAGACATTTGCTCAGAAGAGAATCAAATAGTTTCCTCTTATGCTTCTAAAGTCTGTTTTGAGATCGAAGAAGATTATAAAAATCGTCAGTTTCTGGGGCCTGAAGGAAATGTGGATGTTGAGTTGATTGATAAGAGCACAAACAGATACAGCGTTTGGTTCCCCACTGCTGGCTGGTATCTGTGGTCAGCCACAGGCCTCGGCTTCCTGGTAAGGGATGAGGTCACAGTGACGATTGCGTTTGGTTCCTGGAGTCAGCACCTGGCCCTGGACCTGCAGCACCATGAACAGTGGCTGGTGGGCGGCCCCTTGTTTGATGTCACTGCAGAGCCAGAGGAGGCTGTCGCCGAAATCCACCTCCCCCACTTCATCTCCCTCCAAGCAGGTGAGGTGGACGTCTCCTGGTTTCTCGTTGCCCATTTTAAGAATGAAGGGATGGTCCTGGAGCATCCAGCCCGGGTGGAGCCTTTCTATGCTGTCCTGGAAAGCCCCAGCTTCTCTCTGATGGGCATCCTGCTGCGGATCGCCAGTGGGACTCGCCTCTCCATCCCCATCACTTCCAACACATTGATCTATTATCACCCCCACCCCGAAGATATTAAGTTCCACTTGTACCTTGTCCCCAGCGACGCCTTGCTAACAAAGGCGATAGATGATGAGGAAGATCGCTTCCATGGTGTGCGCCTGCAGACTTCGCCCCCAATGGAACCCCTGAACTTTGGTTCCAGTTATATTGTGTCTAATTCTGCTAACCTGAAAGTAATGCCCAAGGAGTTGAAATTGTCCTACAGGAGCCCTGGAGAAATTCAGCACTTCTCAAAATTCTATGCTGGGCAGATGAAGGAACCCATTCAACTTGAGATTACTGAAAAAAGACATGGGACTTTGGTGTGGGATACTGAGGTGAAGCCAGTGGATCTCCAGCTTGTAGCTGCATCAGCCCCTCCTCCTTTCTCAGGTGCAGCCTTTGTGAAGGAGAACCACCGGCAACTCCAAGCCAGGATGGGGGACCTGAAAGGGGTGCTCGATGATCTCCAAGACAATGAGGTTCTTACTGAGAATGAGAAGGAGCTGGTGGAGCAGGAAAAGACACGGCAGAGCAAGAATGAGGCCTTGCTGAGCATGGTGGAGAAGAAAGGGGACCTGGCCCTGGACGTGCTCTTCAGAAGCATTAGTGGAAGGGACCCTTACCTCGTGTCCTATCTTAGACAGCAGAATTTGTAAAATGAGTCAGTTAGGTAGTCTGGAAGAGAGAATCCAGCGTTCTCATTGGAAATGGATAAACAGAAATGTGATCATTGATTTCAGTGTTCAAGACAGAAGAAGACTGGGTAACATCTATCACACAGGCTTTCAGGACAGACTTGTAACCTGGCATGTACCTATTGACTGTATCCTCATGCATTTTCCTCAAGAATGTCTGAAGAAGGTAGTAATATTCCTTTTAAATTTTTTCCAACCATTGCTTGATATATCACTATTTTATCCATTGACATGATTCTTGAAGACCCAGGATAAAGGACATCCGGATAGGTGTGTTTATGAAGGATGGGGCCTGGAAAGGCAACTTTTCCTGATTAATGTGAAAAATAATTCCTATGGACACTCCGTTTGAAGTATCACCTTCTCATAACTAAAAGCAGAAAAGCTAACAAAAGCTTCTCAGCTGAGGACACTCAAGGCATACATGATGACAGTCTTTTTTTTTTTTGTATGTTAGGACTTTAACACTTTATCTATGGCTACTGTTATTAGAACAATGTAAATGTATTTGCTGAAAGAGAGCACAAAAATGGGAGAAAATGCAAACATGAGCAGAAAATATTTTCCCACTGGTGTGTAGCCTGCTACAAGGAGTTGTTGGGTTAAATGTTCATGGTCAACTCCAAGGAATACTGAGATGAAATGTGGTAAATCAACTCCACAGAACCACCAAAAAGAAAATGAGGGTAATTCAGCTTATTCTGAGACAGACATTCCTGGCAATGTACCATACAAAAAATAAGCCAACTCTGACATTTGGATTCTACCATAGACTCTGTCATTTTGTAGCCATTTCAGCTGTCTTTTGATTAATGTTTTCGTGGCACACATATTTCCATCCTTTTATGTTTAATCTGTTTAAAACAAGTTCCTAGTAGACACCAAAAAAAAAAAAAAAACTCTCCAGCGCTGGATCCGGCCATAAGGGCCTGATCCGTCGAGGGGGGGCCCGGTACCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTTCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGC

Vector Information:

Vector Name:pBluescriptR
Type:bacterial plasmid
Description:Phagemid cloning system T7 and T3 Promoters M13 forward and M13 reverse sequencing primers Ampicillin resistance in bacterial cultures
Properties:loxP
Comments:Modified pBluescript from Riken.
Map:pBluescriptR.gif
Sequence:pBluescriptR.txt
Size (bp):3178
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha N

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
MGC Consortium