Clone: HsCD00329184

Clone ID:HsCD00329184
Is Verified:Y
Status:AVAILABLE
Source:MGC
Description:
Comments:
Type:cDNA
Verification Method:Sequence Verification
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

Original Clone ID MGC:23744
IMAGE ID 4104992
GI 20380963
GenBank Accession BC028673

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 1224 Homo sapiens CLOSED 7336 UBE2V2 ubiquitin-conjugating enzyme E2 variant 2
Insert1
Size (bp)1224
SpeciesHomo sapiens
FormatCLOSED
Tissue Source
Species Specific ID 7336
Gene SymbolUBE2V2
Gene Nameubiquitin-conjugating enzyme E2 variant 2
Keyword

Insert Sequence:

GGGGCGTCGGGCTGCAGGAGAAGATGGCGGTCTCCACAGGAGTTAAAGTTCCTCGTAATTTTCGCTTGTTGGAAGAACTTGAAGAAGGACAAAAAGGAGTAGGCGACGGTACAGTTAGCTGGGGCCTTGAAGATGATGAAGATATGACACTTACAAGGTGGACAGGCATGATTATTGGGCCACCAAGGACAAATTATGAAAACAGAATATATAGCCTGAAAGTAGAATGTGGACCTAAATACCCAGAAGCTCCTCCGTCAGTTAGATTTGTAACAAAAATTAATATGAACGGAATAAATAATTCCAGTGGGATGGTGGATGCCCGGAGCATACCAGTGTTAGCAAAATGGCAAAATTCATATAGCATTAAAGTTGTACTTCAAGAGCTAAGACGTCTAATGATGTCCAAAGAAAATATGAAGCTTCCACAGCCACCAGAAGGACAAACATACAACAATTAATTTTAGTGGATCTCAAACTTGTCTTAAATCAACAACCTTCTACTCATGTTAATGTCTTGATTAAATATCACAATGCAAAATACACATTAAGTAAAAGAATTCCAGCTGGTAAACATGACCTGGACATTTGTAAGAATATATTTAATATATGTACACCCATTATGTTTTCAGGTAACAGGAGGAAAAATGCAGCACAATTTTTTTTCTCTTGAAAGGCACTGTCATTTAAACATAAACCTGGAGTACTCGAAATAGAATTCAGGTTTACAAGATGAAAGCGTGTGGAGAAGTGTCAGATGGCAGTGGAAGCATGTGTGTTTCTAAAAAGTAAAAATCTCAAGAAAACAGAAATGGCATGCTTTACCCATCTTACTTAGTGAAAGAGAGCTGCAGTTGAAATTGTTTAAAAAGTAGCAGGTACAATGAATATTGTCACAGATGTGTTAATTTTTGAAGCAATGTGGGTGCTGACTACTAGTAGTATCAAAAATATGTTCAGGATTGTTTTGATACCTGTATTTATAATAAAAAATGTTGGGGGGAGTTGATGAATTCCTGTTAAAAGCTGTTCTTGTGTGTTACATGTAACAGACATGGTAAATATTTGTTTACAGTCTTTGTTTAACAAACCATGCATTTAAGTTTAAGTGAAGTCAACAAAAAGGAAATAGGTGTATGGATATGTGATTTTGAGATTAAAGTTAGTCTTAAAATGTAAATAAAATGTGAAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Insert Property: Insert 1

Type Value Extra Information
CDS Start 24
CDS Stop 461

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

AGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTACGCGTGTAAAACGACGGCCAGTAGATCTGTAATACGACTCACTATAGGGCGCTAGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTCGACGGTACCGGACATATGCCCGGGAATTCGGCCATTACGGCCGGGGGGGGCGTCGGGCTGCAGGAGAAGATGGCGGTCTCCACAGGAGTTAAAGTTCCTCGTAATTTTCGCTTGTTGGAAGAACTTGAAGAAGGACAAAAAGGAGTAGGCGACGGTACAGTTAGCTGGGGCCTTGAAGATGATGAAGATATGACACTTACAAGGTGGACAGGCATGATTATTGGGCCACCAAGGACAAATTATGAAAACAGAATATATAGCCTGAAAGTAGAATGTGGACCTAAATACCCAGAAGCTCCTCCGTCAGTTAGATTTGTAACAAAAATTAATATGAACGGAATAAATAATTCCAGTGGGATGGTGGATGCCCGGAGCATACCAGTGTTAGCAAAATGGCAAAATTCATATAGCATTAAAGTTGTACTTCAAGAGCTAAGACGTCTAATGATGTCCAAAGAAAATATGAAGCTTCCACAGCCACCAGAAGGACAAACATACAACAATTAATTTTAGTGGATCTCAAACTTGTCTTAAATCAACAACCTTCTACTCATGTTAATGTCTTGATTAAATATCACAATGCAAAATACACATTAAGTAAAAGAATTCCAGCTGGTAAACATGACCTGGACATTTGTAAGAATATATTTAATATATGTACACCCATTATGTTTTCAGGTAACAGGAGGAAAAATGCAGCACAATTTTTTTTCTCTTGAAAGGCACTGTCATTTAAACATAAACCTGGAGTACTCGAAATAGAATTCAGGTTTACAAGATGAAAGCGTGTGGAGAAGTGTCAGATGGCAGTGGAAGCATGTGTGTTTCTAAAAAGTAAAAATCTCAAGAAAACAGAAATGGCATGCTTTACCCATCTTACTTAGTGAAAGAGAGCTGCAGTTGAAATTGTTTAAAAAGTAGCAGGTACAATGAATATTGTCACAGATGTGTTAATTTTTGAAGCAATGTGGGTGCTGACTACTAGTAGTATCAAAAATATGTTCAGGATTGTTTTGATACCTGTATTTATAATAAAAAATGTTGGGGGGAGTTGATGAATTCCTGTTAAAAGCTGTTCTTGTGTGTTACATGTAACAGACATGGTAAATATTTGTTTACAGTCTTTGTTTAACAAACCATGCATTTAAGTTTAAGTGAAGTCAACAAAAAGGAAATAGGTGTATGGATATGTGATTTTGAGATTAAAGTTAGTCTTAAAATGTAAATAAAATGTGAAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTGTCGGCCGCCTCGGCCCTCGAGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCCAGTAGGTAAGTGAACATGGTCATAGCTGTTTCCTAGGAGATCCTGGTCATGACTAGTGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAGATCCATAACTTCGTATAGCATACATTATACGAAGTTATGCGGCCGCGACGTCAATGCCAATAGGATATCGGCATTTTCTTTTGCGTTTTTATTTGTTAACTGTTAATTGTCCTTGTTCAAGGATGCTGTCTTTGACAACAGATGTTTTCTTGCCTTTGATGTTCAGCAGGAAGCTAGGCGCAAACGTTGATTGTTTGTCTGCGTAGAATCCTCTGTTTGTCATATAGCTTGTAATCACGACATTGTTTCCTTTCGCTTGAGGTACAGCGAAGTGTGAGTAAGTAAAGGTTACATCGTTAGGATCAAGATCCATTTTTAACACAAGGCCAGTTTTGTTCAGCGGCTTGTATGGGCCAGTTAAAGAATTAGAAACATAACCAAGCATGTAAATATCGTTAGACGTAATGCCGTCAATCGTCATTTTTGATCCGCGGGAGTCAGTGAACAGGTACCATTTGCCGTTCATTTTAAAGACGTTCGCGCGTTCAATTTCATCTGTTACTGTGTTAGATGCAATCAGCGGTTTCATCACTTTTTTCAGTGTGTAATCATCGTTTAGCTCAATCATACCGAGAGCGCCGTTTGCTAACTCAGCCGTGCGTTTTTTATCGCTTTGCAGAAGTTTTTGACTTTCTTGACGGAAGAATGATGTGCTTTTGCCATAGTATGCTTTGTTAAATAAAGATTCTTCGCCTTGGTAGCCATCTTCAGTTCCAGTGTTTGCTTCAAATACTAAGTATTTGTGGCCTTTATCTTCTACGTAGTGAGGATCTCTCAGCGTATGGTTGTCGCCTGAGCTGTAGTTGCCTTCATCGATGAACTGCTGTACATTTTGATACGTTTTTCCGTCACCGTCAAAGATTGATTTATAATCCTCTACACCGTTGATGTTCAAAGAGCTGTCTGATGCTGATACGTTAACTTGTGCAGTTGTCAGTGTTTGTTTGCCGTAATGTTTACCGGAGAAATCAGTGTAGAATAAACGGATTTTTCCGTCAGATGTAAATGTGGCTGAACCTGACCATTCTTGTGTTTGGTCTTTTAGGATAGAATCATTTGCATCGAATTTGTCGCTGTCTTTAAAGACGCGGCCAGCGTTTTTCCAGCTGTCAATAGAAGTTTCGCCGACTTTTTGATAGAACATGTAAATCGATGTGTCATCCGCATTTTTAGGATCTCCGGCTAATGCAAAGACGATGTGGTAGCCGTGATAGTTTGCGACAGTGCCGTCAGCGTTTTGTAATGGCCAGCTGTCCCAAACGTCCAGGCCTTTTGCAGAAGAGATATTTTTAATTGTGGACGAATCGAACTCAGGAACTTGATTTTTTCATTTTTTTGCTGTTCAGGGATTTGCAGCATATCATGGCGTGTAATATGGGAAATGCCGTATGTTTCCTTATATGGCTTTTGGTTCGTTTCTTTCGCAAACGCTTGAGTTGCGCCTCCTGCCAGCAGTGCGGTAGTAAAGGTTAATACTGTTGCTTGTTTTGCAAACTTTTTGATGTTCATCGTTCATGTCTCCTTTTTTATGTACTGTGTTAGCGGTCTGCTTCTTCCAGCCCTCCTGTTTGAAGATGGCAAGTTAGTTACGCACAATAAAAAAAGACCTAAAATATGTAAGGGGTGACGCCAAAGTATACACTTTGCCCTTTACACATTTTAGGTCTTGCCTGCTTTATCAGTAACAAACCCGCGCGATTTACTTTTCGACCTCATTCTATTAGACTCTCGTTTGGATTGCAACTGGTCTATTTTCCTCTTTTGTTTGATAGAAAATCATAAAAGGATTTGCAGACTACGGGCCTAAAGAACTAAAAAATCTATCTGTTTCTTTTCATTCTCTGTATTTTTTATAGTTTCTGTTGCATGGGCATAAAGTTGCCTTTTTAATCACAATTCAGAAAATATCATAATATCTCATTTCACTAAATAATAGTGAACGGCAGGTATATGTGGCGCGCCTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAA

Sequencing Information:

Phred Score: 894
Date: 2018-02-26

Last Passing Sequencing Read:

NNNNNNAGATACTATAGGGCTGCTCGCGCGCCGACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTCGACGGTACCGGACATATGCCCGGGAATTCGGCCATTACGGCCGGGGCGTCGGGCTGCAGGAGAAGATGGCGGTCTCCACAGGAGTTAAAGTTCCTCGTAATTTTCGCTTGTTGGAAGAACTTGAAGAAGGACAAAAAGGAGTAGGCGACGGTACAGTTAGCTGGGGCCTTGAAGATGATGAAGATATGACACTTACAAGGTGGACAGGCATGATTATTGGGCCACCAAGGACAAATTATGAAAACAGAATATATAGCCTGAAAGTAGAATGTGGACCTAAATACCCAGAAGCTCCTCCGTCAGTTAGATTTGTAACAAAAATTAATATGAACGGAATAAATAATTCCAGTGGGATGGTGGATGCCCGGAGCATACCAGTGTTAGCAAAATGGCAAAATTCATATAGCATTAAAGTTGTACTTCAAGAGCTAAGACGTCTAATGATGTCCAAAGAAAATATGAAGCTTCCACAGCCACCAGAAGGACAAACATACAACAATTAATTTTAGTGGATCTCAAACTTGTCTTAAATCAACAACCTTCTACTCATGTTAATGTCTTGATTAAATATCACAATGCAAAATACACATTAAGTAAAAGAATTCCAGCTGGTAAACATGACCTGGACATTTGTAAGAATATATTTAATATATGTACACCCATTATGTTTTCAGGTAACAGGAGGAAAAATGCAGCACAATTTTTTTTCTCTTGAAAGGCACTGTCATTTAAACATAAACCTGGAGTACTCGAAATAGAATTCAGGTTTACAAGATGAAAGCGTGTGGAGAAGTGTCAGATGGCAGTGGAAGCATGTGTGTTTCTAAAAAGTAAAATCTCAAGAAACAGAAATGGCATGCTTTACCCATCTTACTTAGTGAAGAGAGCTGCAGTTGAAATTGTTAAAAAGTAGCAGGTACATGAAATATGTCACGATGTGTTAATTTTGAAGCATGTGGTGCTGACTCTAGTATATCAAAATATGTCAGGAATGTTTGATACTGTATTNATATAAAAATGTGGGGGAGTGATGATCCTGTAAAGCTGTCTGNNGGTATGTACAGACTGGAATATTGTTACAGCTTGTTACCACCTGCATAAGTTAGTGATCACAAAGAATAGGGTGGATTGATTGCAATAGTGCCTCAAGGAAAATGGAACCAAAAAAAAAAAAAAAACTGGGGCCCGCCCAATTTTAATCTTTGCCGGCCAGAGAAAGCCAGTTCTAAACGGGAAGTGGTCCAAAACCGGGCCCTTAAAAAGGGGGTTCTCTCAACGCAAACCCCCCCCCTTATTTTTAACAAAAAAACCCCCCTTTTANNNNNNNNCCCTGGGGG

Vector Information:

Vector Name:pDNR-LIB
Type:bacterial plasmid
Description:LoxP, multiple cloning site clone; chloramphenicol resistance in bacteria
Properties:
Comments:
Map:pdnr-lib.gif
Sequence:pDNR_LIB.txt
Size (bp):4200
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha N

Antibiotic Selections:

Host Type Marker
bacterial chloramphenicol

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 30 ug/mL chloramphenicol Growth at 37 degrees C in LB supplemented with chloramphenicol (30 ug/mL)

Authors:

Author Name Author Type
MGC Consortium