Clone: HsCD00324647

Clone ID:HsCD00324647
Is Verified:Y
Status:AVAILABLE
Source:MGC
Description:
Comments:
Type:cDNA
Verification Method:Sequence Verification
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

GI 13543963
IMAGE ID 3506309
GenBank Accession BC006121
Original Clone ID MGC:13000

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 1369 Homo sapiens CLOSED 84883 AIFM2 apoptosis-inducing factor, mitochondrion-associated, 2
Insert1
Size (bp)1369
SpeciesHomo sapiens
FormatCLOSED
Tissue Source
Species Specific ID 84883
Gene SymbolAIFM2
Gene Nameapoptosis-inducing factor, mitochondrion-associated, 2
Keyword

Insert Sequence:

GGCCGACAGTGCCTGATTTGAGATGGGGTCCCAGGTCTCGGTGGAATCGGGAGCTCTGCACGTGGTGATTGTGGGTGGGGGCTTTGGCGGGATCGCAGCAGCCAGCCAGCTGCAGGCCCTGAACGTCCCCTTCATGCTGGTGGACATGAAGGACTCCTTCCACCACAATGTGGCTGCTCTCCGAGCCTCCGTGGAGACAGGGTTCGCCAAAAAGACATTCATTTCTTACTCGGTGACTTTCAAGGACAACTTCCGGCAGGGGCTAGTAGTGGGGATAGACCTGAAGAACCAGATGGTGCTGCTGCAGGGTGGCGAGGCCCTGCCCTTCTCTCATCTTATCCTGGCCACGGGCAGCACTGGGCCCTTCCCGGGCAAGTTTAATGAGGTTTCCAGCCAGCAGGCCGCTATCCAGGCCTATGAGGACATGGTGAGGCAGGTCCAGCGCTCACGGTTCATCGTGGTGGTGGGAGGAGGCTCGGCTGGAGTGGAGATGGCAGCAGAGATTAAAACAGAATATCCTGAGAAAGAGGTCACTCTCATTCACTCCCAAGTGGCCCTGGCTGACAAGGAGCTCCTGCCCTCCGTCCGGCAGGAAGTGAAGGAGATCCTCCTCCGGAAGGGCGTGCAGCTGCTGCTGAGTGAGCGGGTGAGCAATCTGGAGGAGCTGCCTCTCAATGAGTATCGAGAGTACATCAAAGTGCAGACGGACAAAGGCACAGAGGTGGCCACCAACCTGGTGATTCTCTGCACCGGCATCAAGATCAACAGCTCCGCCTACCGCAAAGCATTTGAGAGCAGACTAGCCAGCAGTGGTGCTCTGAGAGTGAACGAGCACCTCCAGGTGGAGGGCCACAGCAACGTCTACGCCATTGGTGACTGTGCCGACGTGAGGACGCCCAAGATGGCCTATCTTGCCGGCCTCCACGCCAACATCGCCGTGGCCAACATCGTCAACTCTGTGAAGCAGCGGCCTCTCCAGGCCTACAAGCCGGGTGCACTGACGTTCCTCCTGTCCATGGGGAGAAATGACGGTGTGGGCCAAATCAGTGGCTTCTATGTGGGCCGGCTCATGGTTCGGCTGACCAAGAGCCGGGACCTGTTCGTCTCTACGAGCTGGAAAACCATGAGGCAGTCTCCACCTTGATGGAGAGGCCAGGCGGGAGAACTACCGCAGCAGGTGGGCGTACGGACTGCTTGGCGCATGGCACCCGCCTGGCAAGTGCTAGAACTAATGCTATTCTTCTGGAATAAGATGCCAATGATGTGGTGGCTAGAAATGCAACTTGTATAAAACAAAAATGGGAGAGAGAGAGGTATTAAACAAATACCCCCCTTAGAGGATAAAAAAAAAAAAAAAAAAAAAAAAAAA

Insert Property: Insert 1

Type Value Extra Information
CDS Start 23
CDS Stop 1144

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

CTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGATCACAACAAAAAGCCCGCTCATTAGGCGGGCTAAATTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCGGCCGCTAGCTTTAATGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAGTTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTGGTACGTCGACGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAACTCGAAGGATCCACAAGTTTGTACAAAAAAGCAGGCTTGTAAAACGACGGCCAGTAACTATAACGGTCCTAAGGTAGCGAGGCCTGGGTGGCGAATTCGGCACGAGGGGCCGACAGTGCCTGATTTGAGATGGGGTCCCAGGTCTCGGTGGAATCGGGAGCTCTGCACGTGGTGATTGTGGGTGGGGGCTTTGGCGGGATCGCAGCAGCCAGCCAGCTGCAGGCCCTGAACGTCCCCTTCATGCTGGTGGACATGAAGGACTCCTTCCACCACAATGTGGCTGCTCTCCGAGCCTCCGTGGAGACAGGGTTCGCCAAAAAGACATTCATTTCTTACTCGGTGACTTTCAAGGACAACTTCCGGCAGGGGCTAGTAGTGGGGATAGACCTGAAGAACCAGATGGTGCTGCTGCAGGGTGGCGAGGCCCTGCCCTTCTCTCATCTTATCCTGGCCACGGGCAGCACTGGGCCCTTCCCGGGCAAGTTTAATGAGGTTTCCAGCCAGCAGGCCGCTATCCAGGCCTATGAGGACATGGTGAGGCAGGTCCAGCGCTCACGGTTCATCGTGGTGGTGGGAGGAGGCTCGGCTGGAGTGGAGATGGCAGCAGAGATTAAAACAGAATATCCTGAGAAAGAGGTCACTCTCATTCACTCCCAAGTGGCCCTGGCTGACAAGGAGCTCCTGCCCTCCGTCCGGCAGGAAGTGAAGGAGATCCTCCTCCGGAAGGGCGTGCAGCTGCTGCTGAGTGAGCGGGTGAGCAATCTGGAGGAGCTGCCTCTCAATGAGTATCGAGAGTACATCAAAGTGCAGACGGACAAAGGCACAGAGGTGGCCACCAACCTGGTGATTCTCTGCACCGGCATCAAGATCAACAGCTCCGCCTACCGCAAAGCATTTGAGAGCAGACTAGCCAGCAGTGGTGCTCTGAGAGTGAACGAGCACCTCCAGGTGGAGGGCCACAGCAACGTCTACGCCATTGGTGACTGTGCCGACGTGAGGACGCCCAAGATGGCCTATCTTGCCGGCCTCCACGCCAACATCGCCGTGGCCAACATCGTCAACTCTGTGAAGCAGCGGCCTCTCCAGGCCTACAAGCCGGGTGCACTGACGTTCCTCCTGTCCATGGGGAGAAATGACGGTGTGGGCCAAATCAGTGGCTTCTATGTGGGCCGGCTCATGGTTCGGCTGACCAAGAGCCGGGACCTGTTCGTCTCTACGAGCTGGAAAACCATGAGGCAGTCTCCACCTTGATGGAGAGGCCAGGCGGGAGAACTACCGCAGCAGGTGGGCGTACGGACTGCTTGGCGCATGGCACCCGCCTGGCAAGTGCTAGAACTAATGCTATTCTTCTGGAATAAGATGCCAATGATGTGGTGGCTAGAAATGCAACTTGTATAAAACAAAAATGGGAGAGAGAGAGGTATTAAACAAATACCCCCCTTAGAGGATAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAGGCATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGGCACATGGTCATAGCTGTTTCCTGACCCAGCTTTCTTGTACAAAGTGGTAGATCTGCCGGTCTCCCTATAGTGAGTCGTATTAATTTCGATAAGCCAGGTTAACCTGCATTAATGAATCGGCTGCAGTACCCGGGAATTTAACCCGCCTAATGAGCGGGCTTTTTTTTGTGATCCAAAGGAT

Vector Information:

Vector Name:pOTB7
Type:bacterial plasmid
Description:Gateway® or compatible clone with M13 forward and M13 reverse primers. Chloramphenicol resistance in bacteria. More information available at http://genecollections.nci.nih.gov/MGC/Vectors/prot_potb7
Properties:Gateway
Comments:A set of thirty-six MGC libraries was made in this vector by Dr. Gerry Rubin, University of California, Berkeley CA. Briefly, the method was as follows: Poly A+RNA was reverse trancribed using an anchored oligo-dT primer with an Xho I site. Double stranded cDNA was prepared using a cDNA synthesis kit. The double stranded cDNA was ligated to Eco RI adaptors, digested with Xho I, size selected over a column, and ligated into the vector. The pOTB7 vector was constructed by Dr. Michael Brownstein, National Institute of Mental Health, NIH, Bethesda, MD.
Map:potb7.gif
Sequence:pOTB7.txt
Size (bp):1815
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant N

Antibiotic Selections:

Host Type Marker
bacterial chloramphenicol

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 30 ug/mL chloramphenicol Growth at 37 degrees C in LB supplemented with chloramphenicol (30 ug/mL)

Authors:

Author Name Author Type
MGC Consortium